CAS 1135-40-6: 3-(Cyclohexylamino)-1-propanesulfonic acid

3-(Cyclohexylamino)-1-propanesulphonic acid

3-(Cyclohexylamino)-1-propanesulfonic acid (CHAPS) is a detergent with a high affinity for basic proteins that can be used to extract these proteins from biological samples and solubilize them. CHAPS is a type of ampholyte, which means it has both anionic and cationic groups. The strong interaction between the ionic groups and the hydrophobic group makes CHAPS more efficient than other detergents for extracting proteins. In addition, CHAPS has been shown to inhibit chemiluminescence in some cases. This inhibition may be due to the binding of CHAPS to the enzyme luciferase or its substrate luciferin. Structural analysis of CHAPS has revealed that it contains one thiol group (-SH). This group will react with free radicals at low pH, so it is possible that this reaction mechanism contributes to the antimicrobial effects of CHAPS.

CAPS

M01457 - 3-(Cyclohexylamino)propanesulfonic acid

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Used to formulate CAPS buffer, a zwitterionic buffer useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electro transfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. and minimal reactivity with enzymes or proteins, minimal salt effects.This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

CAPS (N-Cyclohexyl-3-aminopropanesulfonic acid) acts as a chemiluminescence enhancer and can be used in the development of deep-sea in situ analyzers.