CAS: 1246302-80-6

Applications 4β-Hydroxy Cholesterol-d7 is a labelled metabolite of Cholesterol. It is formed from Cholesterol by the drug-metabolizing enzyme cytochrome P 450 3A4. A potential ligand for the nuclear receptor LXR and also a new endogenous CYP3A marker. References Breuer, O., et al.: J. Lipid Res., 36, 2275 (1995), Pikuleva, I., et al.: J. Biol. Chem., 273, 18153 (1998), Chawla, A., et al.: Science, 294, 1866 (2001), Bodin, K., et al.: J. Biol. Chem., 276, 38685 (2001),

4²-hydroxycholesterol is a molecule that is metabolized from cholesterol and is present in the blood. It has been shown to be involved in the development of atherosclerosis and cardiovascular disease. It can be measured in the serum or plasma by liquid chromatography-mass spectrometry (LC-MS) methods, which are sensitive and require little sample volume. The LC-MS method measures the molecular ions of 4²-hydroxycholesterol, which are then converted to mass spectra using a mass spectrometer. The mass spectrum is then used to identify the formic acid adducts of 4²-hydroxylated cholestanol molecules. This technique has been used to measure endogenous levels of 4²-hydroxycholesterol in humans and animals with atherosclerosis or other cardiovascular diseases, as well as those who have undergone heart surgery.