CAS: 1355028-82-8

5-Azidouridine is used for nucleotide labelling and promptly reacts via a click reaction with a terminal alkyne or cyclooctyne conjugated to a reporter (fluorophore or biotine). The formed labelled nucleotide has a stable triazole linker. 5-Azidouridine has been used in living cell fluorescent imaging of cancer cells.

Applications Used in a method and kit for labeling nucleic acid in living cells. The labeled nucleic acid can be used for detecting cell proliferation and cell apoptosis, labeling plasmid vector, virus, small interfering RNA and microRNA, and studying early development.