CAS 144110-43-0: 5-Bromo-6-chloro-3-indolyl-D-glucuronide cyclohexylammonium salt

M04074 - 5-Bromo-6-chloro-3-indolyl beta-D-glucuronide cyclohexylammonium salt

5-Bromo-6-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt is a magenta enzyme substrate commonly used in biochemical assays. This compound is a derivative of indole, containing bromine and chlorine atoms at positions 5 and 6, respectively. The glucuronide moiety is attached to the indolyl ring, and the cyclohexylammonium cation serves as a counterion to balance the charge. This substrate is often utilized in the detection and quantification of β-glucuronidase activity, an enzyme involved in the metabolism of glucuronides. Upon cleavage by β-glucuronidase, the substrate produces a magenta-colored product, allowing for easy visualization and measurement of enzyme activity.

5-Bromo-6-chloro-3-indolyl-β-D-glucuronide cyclohexyl ammonium salt (1:1)

Chromogenic substrate for β-D-glucuronidase (GUS) yielding a magenta precipitate. It is used fordetection of GUS+ bacterial colonies as analternative to 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (X-GlcA).