CAS: 1527467-07-7

27-Alkyne cholesterol is a hydrophobic molecule that is synthesized by the biosynthetic pathway starting from 27-hydroxycholesterol. This molecule is found in the membranes of cells and it can be transported across membranes. 27-Alkyne cholesterol can interact with other molecules, including soluble proteins, through an optical calibrated measurement technique. The molecular interactions are analyzed by simulations that rationalize the data obtained from the optical measurements. It has been observed that this compound has gene regulatory effects on intestinal cells as well as on gene expression in the phospholipid bilayer. The repressor protein binds to 27-alkynes and prevents transcription of genes involved in cholesterol synthesis.

27-Alkyne Cholesterol, an alkyne derivative of cholesterol, facilitates the investigation of cholesterol metabolism and localization through click chemistry with reporter azides in both fixed and living cells. It serves as a substrate for enzymes across diverse species such as bacteria, yeast, rat, and human.