4-Nitrophenyl-β-D-galactopyranoside is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of β-galactosidase activity. This compound features a galactopyranoside moiety linked to a 4-nitrophenyl group, which provides a chromogenic response upon hydrolysis. When β-galactosidase catalyzes the cleavage of the glycosidic bond, it releases 4-nitrophenol, resulting in a color change that can be quantitatively measured, making it a valuable tool in molecular biology and enzymology. The compound is typically a white to pale yellow solid and is soluble in water and organic solvents, facilitating its use in various laboratory settings. Its molecular structure includes a β-D-galactopyranoside unit, which is a six-membered cyclic form of galactose, contributing to its reactivity with specific enzymes. Safety data should be consulted, as with any chemical, to ensure proper handling and disposal.

4-nitrophenyl-β-D-galactopyranoside

200422-18-0: 4-Nitrophenyl-beta-D-galactopyranoside, p-Nitrophenyl-β-D-Galactopyranoside

4-Nitrophenyl b-D-galactopyranoside hydrate

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

4-Nitrophenyl-β-D-galactopyranoside hydrate, 98+%

β-D-Galactopyranoside, 4-nitrophenyl, monohydrate (9CI)

4-Nitrophenyl b-D-galactopyranoside hydrate is a water-soluble pNP-based enzyme substrate ideal for quantifying b-galactosidase activity in a variety of sample types. This chromogenic substrate features a unique hydrate composition, which allows for enhanced solubility and stability when compared to standard pNP-galactosides. With its high sensitivity and robust performance, this substrate is widely used in biotechnology, biochemistry, and molecular biology fields, including the investigation of enzyme kinetics, characterization of glycoside hydrolase specificity, and assessment of gene expression levels.

CAS 200422-18-0: 4-nitrophenyl-β-D-galactopyranoside

4-Nitrophenyl b-D-galactopyranoside hydrate

Ref: 7W-GC0669

4-Nitrophenyl-β-D-galactopyranoside hydrate, 98+%

Ref: 02-L12911

β-D-Galactopyranoside, 4-nitrophenyl, monohydrate (9CI)

Ref: IN-DA002CR5

4-Nitrophenyl b-D-galactopyranoside hydrate

Ref: 3D-EN04981