CAS 207738-08-7: 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hydrate

3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

HRP and TMB - it takes two to tango! The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays. The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.

HRP and TMB - it takes two to tango! The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays. The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.

3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

TMB(dihydrochloride x.hydrate) is the dihydrochloride salt and hydrate form of TMB. It is a non-toxic and non-mutagenic colorless dye used as a chromogenic agent in horseradish peroxidase-based assay systems. This compound also serves as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce a blue oxidized product. Additionally, TMB(dihydrochloride x.hydrate) can detect singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.