CAS 287476-11-3: 1,3,5-triazine-2,4,6-triamine

Applications Isotope labelled Melamine (M208703), which forms synthetic resins with formaldehyde. It remains largely non-metabolized in male Wistar rats with 90% of the administered dose excreted in the urine (1,2) and thus acts as a non-protein nitrogen food additive found in milk (3). Drinking water contaminant candidate list 3 (CCL 3) compound as per United States Environmental Protection Agency (EPA), environmental, and food contaminants. References 1. Mast, R. et al.: Food Chem Toxicol. 1983 Dec;21(6):807-10.2. Smith, J. et al.: Toxicol Appl Pharmacol. 1994 Apr;125(2):296-308.3. Anirudhan, T. et al.: Food Chem. 2017 Jul 15;227:85-92.

Melamine is a chemical compound that can be found in many different types of products. It is used as a flame retardant, plasticizer, and in the production of polyurethane foam. Melamine is also used for its nitrogen content to produce melamine-formaldehyde resin and melamine-urea resins. The 15N3 isotope has been added to melamine molecules to allow for the determination of melamine concentrations in urine and tissue samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The addition of this isotope allows for the distinction between natural and synthetic sources of melamine. This method is a quantitative technique that uses an acidic mobile phase with a linear calibration curve. The matrix effect can be corrected by using hydrochloric acid as a volatile ionization reagent. The detection limit for this technique is 0.1 µg/mL. Chemical ionization is employed with mass spectrometric detection, which can distinguish between natural