P-nitrophenyl-beta-D-galacturonide is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of pectinase and related enzymes. It is a derivative of galacturonic acid, which is a component of pectin, a polysaccharide found in plant cell walls. The presence of the p-nitrophenyl group allows for colorimetric detection, as the compound releases p-nitrophenol upon hydrolysis by specific enzymes, resulting in a measurable yellow color change. This characteristic makes it valuable in enzymatic studies and assays. The compound is typically a white to pale yellow solid and is soluble in water and organic solvents, depending on the pH. Its molecular structure includes a beta-glycosidic bond, which is crucial for its reactivity with glycosidases. Safety data indicates that it should be handled with care, as it may pose health risks if ingested or inhaled. Overall, P-nitrophenyl-beta-D-galacturonide serves as an important tool in enzymology and plant biochemistry research.

P-nitrophenyl-beta-D-galacturonide

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4-Nitrophenyl b-D-galactopyranosiduronic acid is a chemiluminescent substrate for the detection of β-galactosidase. It is used for the determination of α-galactosidase activity in urine, feces, and blood. This product can be used as a chromogenic substrate for the detection of β-galactosidase in tissue culture medium. 4-Nitrophenyl b-D-galactopyranosiduronic acid is also a fluorogenic substrate which can be conjugated to an enzyme such as horseradish peroxidase or alkaline phosphatase.

CAS 39031-76-0: P-nitrophenyl-beta-D-galacturonide

4-Nitrophenyl b-D-galactopyranosiduronic acid

Ref: 3D-EN31788