CAS 521-31-3: Luminol

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Luminol (Diogenes reagent)(Diogenes reagent) is a versatile chemical, exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate

Luminol, for Western Blot and ELISA Horseradish peroxidase (HRP), a common marker enzyme for antibodies, catalyses the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive immunoassays in Western Blot and ELISA. Luminescence can be detected at very low levels using sensitive scintillation counters. Cymit Quimica’s manufacturing process for luminol (L-8600) ensures that the highest purity and lot-to-lot consistency are achieved, making it the product of choice for manufacturers of high-end immunological assays. Performance above-average has also been reported for the application of the product in Western blot procedures. Luminol is also routinely used for the detection of bloodstains in criminal investigation. If the mixture comes in contact with hemoglobin, the iron in the hemoglobin accelerates chemiluminescent oxidation of luminol. Please see Luminol (Cat. number L-8601) for more details.

Applications Luminol is a chemiluminescence reagent. Luminol is used for detection of copper, iron, peroxide, cyanides. References Michalet, X., et al.: Chem. Rev., 106, 1785 (2006), Tang, D., et al.: Biosens. Bioelectron., 24, 2125 (2009), Zhou, X., et al.: Anal. Chem. 81, 255 (2009),

Chemiluminescence detection of biological residues

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Luminol

5-Amino-2,3-dihydrophthalazine-1,4-dione

3-Aminophthalhydrazide is used in the detection of copper, iron, peroxides and cyanides. It exhibits chemiluminescence and utilized to measure opsonic and phagocytic function. It acts as a biological sensor and involved in the detection of polymorphonuclear leukocytes response in a patient with a myeloperoxidase deficiency. Further, it is used as a forensic test for blood. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.