CAS 615-28-1: o-Phenylenediamine, dihydrochloride

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

1,2-Phenylenediamine Dihydrochloride is a compound that can be used as an optical sensor. It has been shown to bind to copper chloride in the presence of human serum and hl-60 cells. This binding induces a change in the optical properties of the solution that can be detected with an optical sensor. The binding of 1,2-phenylenediamine dihydrochloride with copper chloride has been studied using X-ray crystal structures and electrochemical impedance spectroscopy. Based on these results, it was determined that the reaction proceeds via a complexation mechanism involving hydrogen bonding between the amine groups and metal ions.