CAS 88404-25-5: 4-(bromomethyl)-6,7-dimethoxy-2H-chromen-2-one

4-Bromomethyl-6,7-dimethoxycoumarin is used as fluorescent labeling reagent for the reversed-phase HPLC separation and detection of carboxylic acids. It is also used as derivatization reagent: for quantitation of clofibric, bezafibric and fenofibric acids in liver by liquid chromatography, in determination of the concentration of tripeptide glutathione by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and for determination of carboxylic acids in chromatographic detection. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

4-(Bromomethyl)-6,7-dimethoxycoumarin (4-BMDC) is a fluorescent derivative of 6,7-dimethoxycoumarin. 4-BMDC has been used as a marker for the detection of p-nitrophenyl phosphate in urine samples. The analytical method involves the use of matrix-assisted laser desorption ionization time of flight mass spectrometry and monoclonal antibodies. 4-BMDC binds to sarcoplasmic proteins and carboxylate groups on rat liver microsomes with high affinity. It also reacts with tryptophan residues on proteins in human serum.

4-Bromomethyl-6,7-dimethoxycoumarin serves as a fluorescent labeling agent for carboxylic acids during chromatographic analysis [1].

Applications A fluorescent labeling reagent for the reversed-phase HPLC separation and detection of carboxylic acids. References J. Chromatogr., 269, 81 (1983), J. Chromatogr., 309, 403 (1984),

4-(Bromomethyl)-6,7-dimethoxycoumarin