CAS 9001-05-2: Catalase

A proprietary potent and stable alternative to chemical neutralizers such as pyruvate - for the utilization in environmental monitoring applications. Use of catalase as a supplement in media plates offers a more effective and reliable solution for safe and secure environmental monitoring of sterilization in clean rooms, isolators or production facilities by also removing remaining traces and spots of high concentration of H2O2. Currently, agar plates for monitoring disinfection or sterilization are supplemented with pyruvate, which is consumed during the neutralization of hydrogen peroxide. One of the main benefits of this catalase is its high stability in agar media at 50°C, which allows easy preparation of media and processing into plates. Due to the high stability no special storage conditions are needed and a shelf-life of > 6 months at 4-25°C can be guaranteed. Key features are: easy to use formulation complete biocatalytic inactivation of hydrogen peroxide (H2O2) a potent, stable alternative to chemical neutralizers more reliable than pyruvate secure and effective degradation with up to 10% H2O2 concentration This product is a freeze-dried powder formulation. Liquid formulations and custom formulations are available on request.

A catalase product which neutralizes hydrogen peroxide at much higher concentrations (>10 %) compared to pyruvate, which is only capable of completely neutralizing hydrogen peroxide at the lowest concentration tested (2 %). Catalase (EC 1.11.1.6) catalyzes the following reaction:2H2O2 → 2H2O + O21 unit of catalase will decompose 1.0 μmole of H2O2 per min at pH 7.0 and 25 °C.

Catalase, an enzyme, metabolizes H2O2 and ROS; its expression and localization change significantly in tumors.

Enzyme involved in the reduction of hydrogen peroxide to water and oxygen. This is a highly important reaction as it protects the cell from oxidative damage.