Pyruvate oxidase is an enzyme that plays a crucial role in cellular metabolism, particularly in the oxidative decarboxylation of pyruvate to produce acetyl-CoA and carbon dioxide, while also generating reducing equivalents in the form of NADH. This enzyme is typically found in various microorganisms and is involved in the metabolic pathways that convert carbohydrates into energy. Pyruvate oxidase is characterized by its dependence on cofactors, such as thiamine pyrophosphate, and its activity is influenced by factors like pH and temperature. The enzyme catalyzes the reaction in an aerobic environment, making it essential for organisms that rely on aerobic respiration. Its structure often includes a complex arrangement of amino acids that facilitate substrate binding and catalysis. Additionally, pyruvate oxidase is of interest in biotechnology and metabolic engineering due to its potential applications in biofuel production and the synthesis of valuable metabolites. Overall, pyruvate oxidase is a vital enzyme in energy metabolism, contributing to the efficient utilization of pyruvate in various biological systems.

pyruvate oxidase

9001-96-1: 2-Oxopropanoic acid oxidase, E.C. 1.2.3.3, Oxidasa, Piruvato, Oxidase, Pyruvat, Oxidase, pyruvate, Oxydase, Pyruvate, Pyruvic acid oxidase, Pyruvic oxidase

Pyruvate oxidase is a phosphorylating oxidoreductase which uses FAD as a cofactor to convert pyruvate into acetate and CO2. Pyruvate oxidase (PoxB) is used in metabolism studies in bacteria and in clinical assays for pyruvate, and requires thiamine pyrophosphate (TPP) and magnesium alongside FAD for optimum activity.

Activity unit definition: One unit will produce 1.0 μmole of H2O2 per minute during conversion of pyruvate and pyrophosphate to acetylphosphate and CO2 at pH 5.7 and 37 °C.

Pyruvate oxidase from microorganisms

Pyruvate oxidase (PoxB) is a thiamine pyrophosphate-dependent oxidase, a key enzyme in glucose metabolism.

CAS 9001-96-1: pyruvate oxidase

Pyruvate oxidase from microorganisms

Ref: 3D-JAA00196

Pyruvate oxidase

Ref: TM-T76143

Pyruvate Oxidase

Ref: 7W-GZ4747