Dihydrofolate reductase (DHFR) from bovine liver is an enzyme that plays a crucial role in the folate metabolism pathway, specifically in the reduction of dihydrofolate to tetrahydrofolate, a vital cofactor in the synthesis of nucleic acids and amino acids. This enzyme is a key target for certain antifolate drugs, which inhibit its activity to disrupt DNA synthesis in rapidly dividing cells, making it significant in cancer treatment and antimicrobial therapy. The enzyme typically exhibits a homodimeric structure and requires NADPH as a cofactor for its reductive activity. DHFR from bovine liver is characterized by its high specificity for its substrates and its ability to function optimally under physiological conditions. The enzyme's activity can be influenced by various factors, including pH, temperature, and the presence of inhibitors or activators. Its study is essential for understanding metabolic pathways and developing therapeutic agents that target folate metabolism in various diseases. The CAS number 9002-03-3 is associated with this enzyme, facilitating its identification in chemical databases.

dihydrofolate reductase F. bovine liver

9002-03-3: Dihydrofolate Reductase bovine liver

Dihydrofolate reductase (DHFR, 1.5.1.3) is a NADP+/NADPH-dependent oxidoreductase, that reduces dihydrofolate to tetrahydrofolate in the following reaction: dihydrofolate + NADPH + H+ ⇌ tetrahydrofolate + NADP+One unit of dihydrofolate reductase will convert 1.0 μmole of dihydrofolic acid into tetrahydrofolic acid in 1 minute at pH 7.5, 22°C and presence of NADPH.

CAS 9002-03-3: dihydrofolate reductase F. bovine liver

Dihydrofolate reductase

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