CAS 9014-06-6: Penicillin amidase

Penicillin amidase (Penicillin acylase) is an enzyme utilized for cleaving the acyl side chains from penicillins.

Penicillin G acylase, 10mg/ml aqueous solution, is a biocatalytic enzyme used in pharmaceutical research and production. This enzyme is typically sourced from various microbial species, predominantly bacteria, through fermentation processes. Its primary mode of action involves the hydrolysis of penicillin G, catalyzing the cleavage of the side chain amide bond to produce 6-aminopenicillanic acid (6-APA), a core building block for the synthesis of a variety of semi-synthetic penicillins. The uses and applications of Penicillin G acylase are extensive within the field of antibiotic modification and production. It is pivotal in the creation of antibiotics with enhanced properties, such as improved efficacy or reduced resistance. Scientists leverage its specificity and efficiency to modify penicillin derivatives, making it a cornerstone in the development of new generations of beta-lactam antibiotics. The enzyme’s role in sustainable production processes also supports green chemistry initiatives by reducing reliance on chemical synthesis and minimizing byproducts.

Hydrolytic enzyme; biocatalyst in production of beta-lactam antibiotics

As a member of the family of hydrolases this enzyme acts on carbon-nitrogen bonds and may be used in the commercial production of semisynthetic penicillin.