CAS 90217-02-0: 5-Cyano-2,3-di-p-tolyltetrazolium chloride

5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride (5CDTT) is a tetrazolium dye that can be used as an indicator of bacterial cell lysis. It binds to the enzyme catalase and inhibits its activity. 5CDTT is also a fluorescent derivative that emits red light when activated by ultraviolet light. 5CDTT has been shown to bind to bacteria and cells in culture, which causes cellular lysis and releases intracellular contents into the surrounding medium. 5CDTT has been shown to inhibit the growth of Gram negative bacteria such as Escherichia coli K-12 and Salmonella typhimurium, but not Gram positive bacteria such as Staphylococcus aureus or Bacillus subtilis.

5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride is a tetrazolium dye used as an indicator of eukaryotic cells in a range or media including waste water resulting in a cyan color. It is commonly used in microbiology laboratories to measure the viability of cells. The redox potentials of 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride are reduced by the presence of active respiratory cells, resulting in fluorescent formazan.

5-Cyano-2,3-ditolyl tetrazolium chloride