5′-Nucleotidase, with the CAS number 9027-73-0, is an enzyme that catalyzes the hydrolysis of nucleotides to nucleosides and inorganic phosphate, playing a crucial role in nucleotide metabolism. This enzyme is typically found in various tissues, including the liver and brain, and is involved in the regulation of nucleotide levels within cells. It exhibits specificity for 5′-nucleotides, such as AMP, GMP, and CMP, and is important in processes such as signal transduction and the recycling of nucleotides. 5′-Nucleotidase is often used in biochemical assays and research applications to study nucleotide metabolism and enzyme kinetics. The enzyme operates optimally at physiological pH and temperature, and its activity can be influenced by various factors, including the presence of metal ions and inhibitors. Understanding the characteristics and functions of 5′-nucleotidase is essential for elucidating its role in cellular processes and potential implications in health and disease.

5′-Nucleotidase

9027-73-0: E.C. 3.1.3.5, EC 3.1.3.5, 5′-Nucleotidase, 5′-Mononucleotidase, Nucleotidase, 5′-

5′-Nucleotidase (CD73), an ectoenzyme and intrinsic membrane glycoprotein, catalyzes the hydrolysis of 5-nucleotides into their corresponding nucleosides [1].

5′-Nucleotidase (EC 3.1.3.5) is an enzyme that catalyzes hydrolysis of 5' nucleotides, removing the phosphate group:  AMP + H2O ⇌ adenosine + Pi  One unit of 5′-Nucleotidase will generate 1.0 μmole of phosphate ions per minute in the presence of AMP under optimal reaction conditions.

CAS 9027-73-0: 5′-Nucleotidase

5′-Nucleotidase

Ref: TM-T76214

5′-Nucleotidase human

Ref: 3D-JAA02773