CAS 9031-11-2: β-Galactosidase

beta-Galactosidase (EC 3.2.1.23, shortly beta-Gal, also know as lactase) catalyses the hydrolysis of beta-d-galactoside in the presence of water to galactose and alcohol, or lactose into glucose and galactose. beta-Gal has a molecular weight of 540,000 and is composed of four identical subunits of MW 135,000, each with an independent active site. The enzyme has divalent metals as cofactors, with chelated Mg2+ ions required to maintain active site conformation. The molecule contains numerous sulfhydryl groups and is glycosylated. This type of enzyme is found widespread in many microorganisms, plants and animals. In E. coli for instance, the beta-Gal protein is encoded by the lacZ gene. The enzyme is often used as a reporter for monitoring gene expression. beta-Gal also has good characteristics when conjugated to antibody molecules or streptavidin for use in ELISA kits. In addition, the enzyme is used to determine lactose in biological fluids and it is employed in food processing operations. Traditionally, beta-Gal activity is tested using the X-Gal or ONPG chromogenic substrates. But with the pioneering AquaSpark® beta-D-galactoside a substrate for beta-Gal detection becomes available that provides a previously unseen sensitivity. See here for futher informaiton on AquaSpark®: AquaSpark Chemiluminescence | Cymit Quimica

beta-Galactosidase (EC 3.2.1.23, shortly beta-Gal, also know as lactase) catalyses the hydrolysis of beta-d-galactoside in the presence of water to galactose and alcohol, or lactose into glucose and galactose. beta-Gal has a molecular weight of 540,000 and is composed of four identical subunits of MW 135,000, each with an independent active site. The enzyme has divalent metals as cofactors, with chelated Mg2+ ions required to maintain active site conformation. The molecule contains numerous sulfhydryl groups and is glycosylated. This type of enzyme is found widespread in many microorganisms, plants and animals. In E. coli for instance, the beta-Gal protein is encoded by the lacZ gene. The enzyme is often used as a reporter for monitoring gene expression. beta-Gal also has good characteristics when conjugated to antibody molecules or streptavidin for use in ELISA kits. In addition, the enzyme is used to determine lactose in biological fluids and it is employed in food processing operations. Traditionally, beta-Gal activity is tested using the X-Gal or ONPG chromogenic substrates. But with the pioneering AquaSpark® beta-D-galactoside a substrate for beta-Gal detection becomes available that provides a previously unseen sensitivity. See here for futher informaiton on AquaSpark®: AquaSpark Chemiluminescence | Cymit Quimica

Enzymes and prepared enzymes, nesoi

β-Galactosidase (GAL) is a glycoside hydrolase that hydrolyzes the β-glycosidic bond formed between galactose and its organic part.

beta-Galactosidase (EC 3.2.1.23, shortly beta-Gal, also know as lactase) catalyses the hydrolysis of beta-d-galactoside in the presence of water to galactose and alcohol, or lactose into glucose and galactose. beta-Gal has a molecular weight of 540,000 and is composed of four identical subunits of MW 135,000, each with an independent active site. The enzyme has divalent metals as cofactors, with chelated Mg2+ ions required to maintain active site conformation. The molecule contains numerous sulfhydryl groups and is glycosylated. This type of enzyme is found widespread in many microorganisms, plants and animals. In E. coli for instance, the beta-Gal protein is encoded by the lacZ gene. The enzyme is often used as a reporter for monitoring gene expression. beta-Gal also has good characteristics when conjugated to antibody molecules or streptavidin for use in ELISA kits. In addition, the enzyme is used to determine lactose in biological fluids and it is employed in food processing operations. Traditionally, beta-Gal activity is tested using the X-Gal or ONPG chromogenic substrates. But with the pioneering AquaSpark® beta-D-galactoside a substrate for beta-Gal detection becomes available that provides a previously unseen sensitivity. See here for futher informaiton on AquaSpark®: AquaSpark Chemiluminescence | Cymit Quimica