Boc-Gly-Gly-Leu-pNA, with the CAS number 98210-71-0, is a synthetic peptide substrate commonly used in biochemical research, particularly in the study of proteolytic enzymes. The "Boc" (tert-butyloxycarbonyl) group serves as a protective group for the amino terminus, enhancing the stability and solubility of the peptide. The sequence consists of two glycine (Gly) residues, a leucine (Leu) residue, and a para-nitroanilide (pNA) moiety, which is a chromogenic group that allows for the quantification of enzymatic activity through colorimetric assays. This compound is typically utilized in assays to measure the activity of serine proteases and other enzymes that cleave peptide bonds. Its characteristics include good solubility in organic solvents and aqueous solutions, making it versatile for various experimental conditions. The presence of the pNA group enables the detection of enzymatic cleavage by producing a measurable color change, facilitating the study of enzyme kinetics and inhibition. Overall, Boc-Gly-Gly-Leu-pNA is a valuable tool in peptide chemistry and enzymology.

Boc-Gly-Gly-Leu-pNA

98210-71-0: Subtilisin A Substrate I, Subtilisin A Substrate I, Colorimetric, L-Leucinamide,N-[(1,1-dimethylethoxy)carbonyl]glycylglycyl-N-(4-nitrophenyl)-

Boc-Gly-Gly-Leu-pNA is an analog of the protease inhibitor serine protease. It has a reactive site that is similar to the reactive site on serine proteases. This enables Boc-Gly-Gly-Leu-pNA to bind to them and inhibit their activity. The compound also inhibits neutrophil activation, as shown by a decrease in its expression of CD11b and CD11c, which are markers of neutrophils.

CAS 98210-71-0: Boc-Gly-Gly-Leu-pNA

Boc-Gly-Gly-Leu-pNA

Ref: 3D-FB110713