Fluorogenic Substrates

This product is a fluorogenic substrate that is used in the detection of enzymes in biological systems. It is also used as a ligand to study enzyme activity and can be used to quantify enzyme activity. This product is made from high-purity, high-quality reagents and is often used as an enzyme substrate in diagnostic kits for food testing, environmental testing, and detection of bacteria with the help of chemiluminescence. The CAS number for this product is 700368-52-1. This product can be used for fluorescence staining and bioluminescence.

2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt is a fluorogenic substrate for alpha-galactosidase. It is used in the analysis of cultured media and as a conjugate for enzyme substrates. This product has been shown to be useful in food testing, diagnostics, and environmental testing. 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt has been used as a chromogenic substrate for the detection of alpha-galactosidase activity. This product is also used as a bioluminescent substrate for the detection of bioluminescence.

Fluorogenic substrate for peroxidase

L-Histidine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for histidine aminopeptidase.

Fluorogenic substrate to assay the activity of neuraminidase. 4-Methylumbelliferone is released from 4-MUNANA by neuraminidase and can be detected fluorometrically with excitation at 365-380 nm and emission at 445-454 nm. Also applicable for fluorescent staining of sialidases in PAGE.

4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.

Fluorogenic substrate for beta-glucuronidase

Resorufin N-acetyl-b-D-galactosamine is a fluorogenic substrate used as a probe to detect and quantify the activity of N-acetyl-beta-D-galactosaminidase (also known as beta-hexosaminidase or HEX(NAG)). Particularly useful in biomedical research, clinical chemistry, and diagnostics, when the substrate is cleaved, the highly fluorescent resorufin dye is produced that can be detected at approximately 598 nm.

Substrate to study the nitroreductase activity in the pathogenic fungus Sporothrix schenckii, a thermal dimorphic pathogenic fungus causing a subcutaneous mycosis.

4-Chloromethyl-6,8-difluoroumbelliferyl b-D-galactopyranoside is a compound commonly used in biochemical research as a fluorescent substrate for the detection of sugar hydrolase enzymes. This compound can be cleaved by enzymes such as β-galactosidase, releasing a fluorescent molecule that can be easily detected using fluorescence spectroscopy. The compound is a member of the family of umbelliferyl glycosides, which have been widely used in the study of various biochemical pathways for many years. The advantage of using this particular substrate is that the fluorescent product has a high quantum yield, which means it produces a strong signal for detection. This substrate has been used in a wide variety of applications, including the study of microbial physiology and in the development of diagnostic assays for detecting bacterial and fungal infections.

4-Methylumbelliferyl propionate is a fluorogenic substrate for esterase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl propionateas a substrate for measuring the esterase activity is used for esterase deficiency screening assays.

Fluorogenic substrate for α-galactosidase yielding a blue fluorescent solution upon cleavage. It is used for species differentiation within the family of Enterobacteriaceae. It is also utilized for the localization of the enzyme in plants.

4-Methylumbelliferyl 3-deoxy-D-glycero-a-D-galacto-2-nonulosonic acid sodium salt is a fluorogenic substrate that can be used to detect enzyme activity. It reacts with the enzyme β-galactosidase, which hydrolyzes lactose in the presence of oxygen and hydrogen peroxide. The product is 4methylumbelliferone, which emits light in the presence of luciferin and an oxidizing agent. This product is used for diagnostics, food testing, and environmental testing. CAS No. 123269-95-4

L-Serine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic peptide substrate for peptidases. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium salt is a fluorogenic substrate for N-acetylgalactosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium saltas a substrate for measuring the N-acetylgalactosamine-6-sulfatase activity is used for diagnosis of Mucopolysaccharidosis IVA (MPS IVA) (a rare lysosomal storage disorder) and Morquio A syndrome screening.

FLsharp™ - Phosphate, disodium salt is a fluorogenic water-soluble substrate for phosphatase enzymes. Its chemical name is 6-Chloro-2-[5-chloro-2-(phosphonooxy)phenyl]-4(1H)-quinazolinone disodium salt and was developed by Molecular Probes in the 1990s. After cleavage of the substrate by phosphatase activity, an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissue sections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as a substrate for bacterial phosphatases.

Colchicine fluorescein is the fluorogenic form of colchicine. It is used for staining assembly of tubulin. Colchicine-site inhibitors (abbrevated as CBSIs) disrupt tubulin polymerization. Colchicine fluorescein serves as a probe to study the binding of colchicine to tubulin, as the colchicine binding site is a promising target for discovering novel antitumour agents.

4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-beta-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for diagnosis of fucosidosis (defficiency of L-fucosidase).

D-Alanine 7-amido-4-methylcoumarin free base is a chromogenic substrate that is used in diagnostic testing. It is used to identify the presence of beta-lactamase, which catalyzes the hydrolysis of penicillin and cephalosporin antibiotics, by turning the substrate into a bright blue color. D-Alanine 7-amido-4-methylcoumarin free base can also be used as a fluorescent substrate for bioluminescence or chemiluminescence reactions. This product is high quality and has CAS number 201847-51-0.

4-Methylumbelliferyl-β-D-xylopyranoside is a fluorescent probe that has been used to visualize the enzyme activities of glycosidases, such as endoglycosidase H and α-amylase. It is also used as a tool in structural analysis of oligosaccharides. 4-Methylumbelliferyl-β-D-xylopyranoside has been shown to inhibit collagenase activity in mice, which may be due to its ability to inhibit protein synthesis and cell division.

4-Methylumbelliferyl 4-deoxychitobiose is a fluorescent substrate used to detect the activity of chitinase. It is a chromogenic substrate that can be used in diagnostic applications and provides a fluorescence response upon hydrolysis. This product is highly pure, with a minimum of 98% purity. It can be used as an enzyme substrate for the detection of chitinase activity or as a ligand for immunoassays.

(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.

4-Methylumbelliferyl sulfate, potassium salt is a metabolite of 4-methylumbelliferone and has been used as a human serum marker. This molecule is conjugated with glucuronide and p-hydroxybenzoic acid to form the sulfate, glucuronide conjugate. The reaction mechanism of this compound is not known, but it is thought that the metabolism may involve oxidation at the methyl group. The analytical method for this compound can be performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This method can also be used in vitro to test antimicrobial activity against bacteria such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.

6,8-Difluoro-4-methylumbelliferyl b-D-glucopyranoside is a versatile fluorogenic substrate suitable for the measurement of various glycosidase enzymes. Its unique structure allows for enhanced sensitivity and selectivity in enzyme assays, making it ideal for studying enzyme kinetics and screening of potential enzyme inhibitors.

Boc-Val-Pro-Arg-PAB-Resorufin is fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex.

Bis-(4-methylumbelliferyl)phosphate, or bis-4MU-phopshate, is a fluorogenic substrate used to study phosphatase activity, particularly in enzyme-linked immunosorbent assays (ELISAs).

6-(b-D-Galactopyranosyloxy)-9-(4-methoxy-2-methylphenyl)-3H-xanthen-3-one (MGBG) is a hydrophilic activatable fluorescence probe that has been shown to be effective in photodynamic therapy. MGBG is activated by light and has a high affinity for esterases, which are found in many tissues. The fluorescence of the MGBG is quenched by the esterase enzymes, but when it is exposed to light and oxygen, the probe becomes activated and its fluorescence increases. MGBG can be used as a fluorescent probe for intraperitoneal tumor resection or as a fluorescent imaging agent in optical microscopy.

N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a Ffluorogenic substrate used in protease activity assays.

4-Methylumbelliferyl acetate is a fluorescent compound that can be used to measure the activity of cytochrome P450 enzymes. The fluorescence intensity of 4-methylumbelliferyl acetate increases when it reacts with substrates and inhibitors in vitro. This chemical has been shown to inhibit the enzyme cyclooxygenase in rat liver microsomes, which may lead to blood disorders. It also inhibits coumarin derivatives and human pathogens, such as erythromycin-resistant Staphylococcus aureus (MRSA). 4-Methylumbelliferyl acetate is used as a substrate for polymerase chain reaction (PCR) analysis because it is not susceptible to hydrolysis by esterases.

4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and glycoprotein metabolism disorder detection.

4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of galactosidases. It has been shown to be a potent inhibitor of these enzymes and can be used to study the activity of various galactosidases. This substrate is used in the measurement of carbohydrate metabolism, including glycolysis and glycogenolysis. 4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside has been shown to produce chemiluminescence when reacted with horseradish peroxidase and hydrogen peroxide. It also produces bioluminescence when exposed to luciferase and adenosine triphosphate (ATP).

4-Methylumbelliferyl a-L-idopyranosiduronic acid is an ester that is used as a marker for the identification of glycolipids. It is a fluorogenic substrate for alpha-L-iduronidase used as a marker for the diagnosis of autoimmune diseases, including diabetic neuropathy. 4-Methylumbelliferyl a-L-idopyranosiduronic acid has been shown to inhibit inflammation and cancer, although it is not currently approved for this use. The mechanism by which 4-methylumbelliferyl a-L-idopyranosiduronic acid inhibits inflammation may be due to its ability to block cation channels on the surface of cells, thereby preventing inflammatory mediators from entering cells.

Resorufin oleate is a fluorogenic lipase substrate.

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside is a fluorescent substrate that is used to measure the activity of the enzyme beta-glucuronidase. It is a chemiluminescent compound that emits light when hydrolyzed by an enzyme. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside has been shown to be a high quality and environmentally friendly product, as it does not cause any significant change in pH or turbidity in water samples. It has also been shown to be nontoxic and nonmutagenic at concentrations up to 400 ppm. This product can be used for a variety of purposes, including diagnostic tests for bacterial infections or food testing.

Fluorogenic substrate for cathepsin B or aminopeptidase III. Yields a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium salt is a fluorogenic substrate for N-sulfoglucosamine sulfohydrolase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium saltas a substrate for measuring the N-sulfoglucosamine sulfohydrolase activity is used for screening of Mucopolysaccharidosis IIIA (MPS IIIA) and diagnosis of Sanfilippo A syndrome.

L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.

4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is a fluorogenic substrate for cellulase. After cleavage, the fluorophore 4-(trifluoromethyl)umbelliferone, commonly referred as TFMU, is released, exhibiting a blueish color. 4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is used to study the enzyme kinetics in bacterial cellulose degradation assays as well as in biomass conversion research studies.

4-Methylumbelliferyl alpha-D-mannopyranoside (4-MU-a-D-Man) is a fluorogenic substrate used in activity assays for alpha-mannosidase. When it is hydrolyzed by alpha-mannosidase, it produces a fluorescent compound, 4-methylumbelliferone (4-MU). 4-MU-a-D-Man is often used in medical research to monitor the activity of alpha-mannosidase, which is important in lysosomal storage diseases.  In addition, 4-Methylumbelliferyl alpha-D-mannopyranoside has been used to study the the activity of concanavalin A, a lectin that specifically binds to alpha-D-mannopyranosyl and alpha-D-glucopyranosyl residues on various glycoproteins. The use of this fluorogenic enzyme substrate permits study the degree of alpha-D-mannosidic linkage cleavage by the enzyme.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. It can be used in diagnostic tests to identify antibodies against certain bacteria and fungi. The fluorogenic form of 4-methylumbelliferyl 2-acetamido-3,4,6-triO acetyl -2 deoxy a D glucopyranoside can be used as a chemiluminescent substrate for detecting bacterial activity. 4 methylumbelliferyl 2 acetamido 3,4,6 triO acetyl -2 deoxy a D glucopyranoside is also useful in bioluminescent assays to detect ATP production in bacteria and other microorganisms. CAS No: 1174287-76-3

4-Methylumbelliferyl 2-O-(a-L-fucopyranosyl)-b-D-galactopyranoside is an enzyme substrate used in chromogenic assays of alpha-fucosidases. It is also used for glycoprocessing enzymes in synthesis.

4-Methylumbelliferyl 2-azido-2-deoxy-4,6-O-phenylmethylene-a-D-galactopyranoside is a fluorogenic substrate that reacts with beta D galactosidase to produce light. This product is useful for diagnostics, conjugate production, and other applications. 4MUF 2 -Gal provides a convenient means of detecting beta D galactosidase activity in cell culture media and can be used as a chromogenic substrate for the detection of enzymes involved in glycosylation reactions. It is also used to detect staining by bioluminescence. The compound can be used in food testing and environmental testing to measure the presence of beta D galactosidase.

4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-b-D-galactopyranosyl-b-D-glucopyranoside is a chemiluminescent substrate that is used in diagnostic tests. This compound has been approved by the FDA and EPA for use in food testing, as well as for the detection of certain bacteria, parasites, and fungi. It is also used to detect protein kinase activity in cells or tissue samples. 4MUG can be conjugated to an enzyme or antibody to produce a fluorescent signal when activated by light at 350 nm. The compound has been shown to be nonfluorescent when in the dark, but will emit fluorescence upon activation with light at 350 nm.

This Clips-O substrate 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin allows the detection of single and dual enzymatic activity (L-alanine aminopetidase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.

Please enquire for more information about L-Pyroglutamic acid 7-amido-4-methylcoumarin including the price, delivery time and more detailed product information at the technical inquiry form on this page

Fluorescent dye substrate for flow cytometric measurements

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase, which hydrolyzes the substrate to produce a fluorescent product. This fluorogenic substrate can be used in chemiluminescent detection of β-galactosidase activity as well as in immunochemical and immunohistochemical analysis of glycoproteins. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is also used for staining of glycoprotein substrates with periodic acid Schiff reagent or Alcian blue, and it can be used in the diagnosis of glycogen storage diseases.

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