Apoptosis

GSK2256098 (GTPL7939) is a small molecule FAK kinase inhibitor.

HLCL-61 hydrochloride (HLCL-61 HCL) is a potent and selective PRMT5 inhibitor for the treatment of acute myeloid leukemia.

Isatin (2,3-Indolinedione) is an endogenous MAO inhibitor.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKR in the samples is then determined by comparing the OD of the samples to the standard curve.

hCAIX-IN-23 (Compound 27) is a human carbonic anhydrase (hCA) inhibitor with Ki values of 10.4 nM for hCA IX and 8.5 nM for hCA XII. It not only inhibits hCA activity but also releases NO, exerting dual anti-tumor effects. By reducing the expression of hCA IX and iron regulatory proteins, hCAIX-IN-23 regulates mitochondrial caspase activity and the ferroptosis pathway, thereby inducing apoptosis. This compound is utilized in research focused on renal cancer.

SIJ1777, a derivative of GNF-7, exhibits potent anticancer effects against melanoma cells harboring BRAFI/II/III mutations. The compound significantly inhibits the activation of MEK, ERK, and AKT. Furthermore, SIJ1777 notably induces apoptosis (cell death) and effectively prevents migration, invasion, and anchorage-independent growth of melanoma cells with BRAFI/II/III mutations.

SKI II is a selective non-ATP S1P receptor inhibitor with an IC50 of 0.5 μM; it doesn't inhibit PKCα, PI3K, or ERK2.

AT9283 (J-504568) is an effective multi-targeted inhibitor of JAK2(IC50=1.2 nM) and JAK3(IC50=1.1 nM), Aurora A, Aurora B and Abl(T315I).

Applications Xanthorrhizol is a nautral sesquiterpenoid from the rhizome of Curcuma xanthorrhiza that induces apoptosis and growth arrest in HCT116 human colon cancer cells. References Kang, Y.J., et al.: J. Pharmacol. Sci., 111, 276 (2009); Cheah, Y.H., et al.: Anticancer. Res., 26, 4527 (2006);

DX3-213B is a potent, orally active inhibitor of oxidative phosphorylation (OXPHOS) complex I with an IC50 of 3.6 nM.

PF-3758309 hydrochloride (PF-03758309 hydrochloride) , is a PAK4 inhibitor, is also a n orally bioavailable small-molecule inhibitor of p21-activated kinase 4 (

Scr-IN-1 (Compound 4e) is a tyrosine kinase inhibitor demonstrating inhibitory activity against HCT-116 and MIA-PaCa-2 cells, with IC50 values of 0.16 μM and 1.16 μM, respectively. It shows selectivity towards HCT-116 cells and MIA-PaCa-2 cells, with a selectivity index (SI) greater than 625 and 86. Scr-IN-1 induces apoptosis in HCT-116 colon cancer cells without altering the proportion of necrotic cells and is a potential novel SRC kinase inhibitor for HCT-116 cells. This compound is suitable for cancer research.

Dihydroartemisinin (Artenimol) is a metabolite of artemisinin.

APPA, an aldose reductase inhibitor, demonstrates efficacy in preventing apoptosis and symptoms of Streptozotocin-induced diabetes in rats through inhibition of

3,4-Dicaffeoylquinic acid (Isochlorogenic acid B) has antioxidant activity.

Stability Hygroscopic Applications A potent and selective ATP-competitive dual inhibitor cdc7/cdk9. It blocks DNA synthesis and affects the phosphorylation of the replicative DNA helicase at Cdc7-dependent phosphorylation sites. Inhibits cell proliferation in a variety of human cell lines and induces apoptosis in a p53-independent manner in vivo. Also inhibits mitogen-activated protein kinase-activated protein kinase-2 (MK-2). References Anderson, D.R. et al.: J. Med. Chem., 50, 2647 (2007); Montagnoli, A. et al.: Nature Chem. Biol., 4, 357 (2008); Charych, D.H. et al.: J Cell. Biochem., 104, 1075 (2008); Jackson, P.K. et al.: Nature Chem. Biol., 4, 331 (2008);

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Paris saponin VII (Dioscini) shows inhibitory effects on cell proliferation.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKCb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKCb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKCb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKCb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Perillyl Alcohol, a monoterpene with antitumor properties, inhibits protein activation blocking cancer cell growth.

Sotigalimab (APX 005) is a monoclonal antibody targeting CD40 with anticancer activity for the study of metastatic pancreatic cancer.

Orsellinic acid is a phenolic acid extracted from lichens.

Almorexant (ACT 078573), a strong dual orexin receptor antagonist, has Ki of 1.3 nM for OX1 and 0.17 nM for OX2.

R406 free base (R406 (free base)) is a potent Syk inhibitor.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in

JG-98 is an allosteric Hsp70 inhibitor, displays high active against the breast cancer cell lines MDA-MB-231 and MCF-7 (EC50s: 0.4/0.7 μM).

Nur77 modulator 1: binds at 3.58 μM, boosts expression, alters location, triggers ER stress/autophagy, induces apoptosis, and fights hepatoma.

Aspirin (Acetylsalicylic Acid) is a COX inhibitor. Aspirin has anti-inflammatory, antipyretic and analgesic activities. Cost-effective and quality-assured.

Degrasyn (WP1130) inhibits DUBs (USP5, UCH-L1, USP9x, USP14, UCH37) and Bcr/Abl without affecting the 20S proteasome.

Alexidine dihydrochloride: antifungal, antibiofilm, targets PTPMT1 for mitochondrial apoptosis, potential cancer treatment.

Tagitinin C is a sesquiterpene compound isolated from Tithonia diversifolia, an inducer of ferroptosis with anti-cancer activity.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFRSF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFRSF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFRSF7 in the samples is then determined by comparing the OD of the samples to the standard curve.

BRD3308 is a highly selective inhibitor of HDAC3(IC50 of 54 nM), attenuating PE-mediated phosphorylation of ERK but not JNK.

Methyl 3,4-dihydroxybenzoate (Protocatechuic acid methyl ester) is a major metabolite of antioxidant polyphenols found in green tea.

Bilobalide ((-)-Bilobalide), a bioactive from Gingko Biloba, is active on hypoxia-induced alterations.

FKBP12 ligand-1 is the target protein ligand for MC-25B, which is a PROTAC targeting FKBP12.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGFBP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

RA-9 is a potent and selective proteasome-associated inhibitor of deubiquitinating enzymes (DUBs), with favorable toxicity profile and anticancer activity.

LB42708 is an orally active farnesyltransferase (FTase) inhibitor (IC50: 0.8/1.2/2.0 nM toward H/N/K-ras).

Bigelovin: RXRα agonist, inhibits mTOR via ROS, induces apoptosis/autophagy, anti-tumor sesquiterpene from Inula sp.

Divalproex Sodium (Valproate semisodium) binds to and inhibits gamma-aminobutyric acid (GABA) transaminase and its anticonvulsant activity may be exerted by

Torin 2: mTOR inhibitor, IC50=0.25 nM, 800x more selective than PI3K, with EC50=28/35/118 nM for ATM/ATR/DNA-PK.

TBHQ (tert-Butylhydroquinone) is an antioxidant that induces an antioxidant response through the redox-sensitive transcription factor Nrf2.

SAR125844, a potent and highly selective inhibitor of the MET receptor tyrosine kinase (RTK), for intravenous administration. (IC50 value of 4.2 nmol/L)

Anticancer Agent 146 (Compound 1.19) acts as a necroptosis inducer and demonstrates anti-tumor efficacy in the MDA-MB-231 mouse xenograft model [1].

Alpha-Hederin (Tauroside E) is a water-soluble pentacyclic triterpenoid saponin found in the seeds of Nigella sativa, with hemolytic and apoptotic properties.

Etanercept is a fusion protein consisting of the soluble portion of the p75-tumor necrosis factor receptor (TNFR) and the Fc fragment of human IgG1 and is commonly used to treat patients with rheumatoid arthritis.Cost-effective and quality-assured.

CM-272 is a dual G9a/DNA methyltransferases (DNMTs) inhibitor.

Tauroursodeoxycholate sodium (TUDC) is an endoplasmic reticulum (ER) stress inhibitor, used for the treatment of gallstones and liver cirrhosis.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Ginkgetin: antiviral, antifungal, anti-inflammatory; COX-2 reducer; STAT3 inhibitor; pro-apoptotic in cancer; neuroprotective in Parkinson's.

1-beta-D-Arabinofuranosylthymine (Thymine 1-β-D-arabinofuranoside) is a purine nucleoside analog.

AMPK-IN-6 (compound 13a) is a potent AMPK inhibitor with an IC50 value of 0.093 µM. It induces apoptosis and suppresses autophagy. Additionally, AMPK-IN-6 exhibits antiproliferative activity and holds potential for research into pulmonary arterial hypertension.

Hydroxyurea (Hydroxycarbamide), an antineoplastic agent, inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.

Mollugin could be a JAK2 inhibitor, anti-inflammatory, chemotherapeutic agent in OSCCs, bone disorder therapy, and modulates HER2 in cancer.

HS-173 is an effective PI3Kα inhibitor (IC50: 0.8 nM).

Polyphyllin D induces apoptosis via the mitochondrial apoptotic pathway as evidenced by decreased Bcl-2 expression levels, disruption of MMP and increased Bax,

Deracoxib (SC 46) is a selective COX-2 inhibitor, non-steroidal, non-narcotic anti-inflammatory drug with an IC50 of 70-150 μM for osteosarcoma cells.

Applications 5-Methoxy-1-(methoxymethoxy)-2-methyl-naphthalene-d3 is an intermediate in the synthesis of Plumbagin-d3 (P627002). Plumbagin-d3 is labelled Plumbagin (P627000) which induces apoptosis in cancer cells. It also inhibits NADPH oxidase 4 in a time- and dose-dependent manner. References Klotz, L., et al.: Molecules, 19 14902 (2014); Gupta, S., et al.: Cancer Metastasis Rev., 29, 405 (2010); Schramm, A., et al.: Vascul. Pharmacol., 56, 216 (2012)

Afatinib Dimaleate (BIBW 2992MA2) is an orally bioavailable anilino-quinazoline derivative and inhibitor of the EGFR family, with antineoplastic activity.

Eupatolide, from Inula japonica flowers, has anti-inflammatory properties and aids in researching TRAIL resistance in breast cancer.

2'-Epi-2'-O-Acetylthevetin B (GHSC-74), a cardiac glycoside extractable from Cerbera manghas L.

Fludarabine (Fludarabinum) is a fluorinated purine analog, an inhibitor of nucleic acid synthesis and an inhibitor of STAT1 activation.

Troglitazone (Romglizone) is a PPARγ agonist with anti-inflammatory and anti-tumor activity.

VER-50589 is a potent HSP90 inhibitor.

MI-1061 is a orally bioavailable inhibitor of MDM2 (MDM2-p53 interaction) (IC50=4.4 nM).

NLRP3-IN-72 (Compound 2) is a benzimidazole derivative. It exhibits anti-inflammatory and antioxidant properties, with an IC50 of 0.3 μM for NLRP3 IL-1β, a PD50 of 0.4 μM for protection against cell pyroptosis, and an EC50 of 0.6 μM for inducing HO-1.