Apoptosis

Tebufenozide is a novel nonsteroidal ecdysone agonist. It shows good efficacy and playing an increasingly important role in the control of Lepidopteran pests.

Thiocolchicine inhibits tubulin polymerization (IC50: 2.5 µM, Ki: 0.7 µM), induces apoptosis, and serves as an ADC cytotoxin.

TD52, an Erlotinib derivative, is an oral CIP2A cancer inhibitor that disrupts Elk1's attachment to the CIP2A promoter.

Ursonic Acid (TOS-BB-0966) belongs to the family of Ursane Triterpenes whose structure is based on the pentacyclic ursane skeleton.

Applications Labelled PAC-1 is a caspase 3 activator. Caspase activation is a key strategy in cancer therapy. Caspase 3 is a key executioner caspase and direct effector of apoptosis. Hallmark of many cancers is the circumvention of signal in the activation of executioner caspases and loss of apoptotic response. EC50 for caspase 3 activation = 0.33 μM and caspase 7= 4.5 μM. The IC50 for apoptosis induction = 0.92 μM. Elevated caspase 3 level in cancerous cell lines and tissues allows PAC-1 to selectively induce apoptosis in tissues. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Slee, E., et al.: J. Biol. Chem., 276, 7320 (2001), Pop, C., et al.: Biochemistry, 42, 12311 (2003), Li, L., et al.: Science, 305, 1471 (2004), Green, D., et al.: J. Clin. Invest., 115, 2610 (2005), Satoh, T., et al.: Clin. Cancer Res., 15, 3872 (2009),

Antitumor agent-115 (SS-12) is a potent anti-tumor compound, exhibiting an IC50 range of 0.34 μM-24.14 μM against the 4T1 mouse breast cancer cell line.

Applications Platycodin D is a known inhibitor of the proliferation of human gastric cancer cells as well as inducing apoptosis. It also causes anti-atherosclerotic. References Wu, J. et al.: Biol. Pharm. Bull. 35, 1216 (2012); Chun, J. et al.: J. Cell. Biochem., 114, 456 (2013);

Stability Hygroscopic Applications An antimitotic agent that disrupts microtubles by binding to tubulin and preventing its polymerization. Stimulates the intrinsic GTPase activity of tubulin. Induces apoptosis in several normal and tumor cell lines and activates the JNK/SAPK signaling pathway. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Lunduena, R.F., et al.: Curr. Opin. Cell Biol., 4, 53 (1992), Ceccatelli, S., et al.: Neuroreport, 8, 3779 (1997), Want, T.H., et al.: J. Biol. Chem., 273, 4928 (1998), Andreu, J.M., et al.: Biochemistry, 37, 8356 (1998), Jordan, A., et al.: Med. Res. Rev., 18, 259 (1998),

NSC-207895 (XI-006) suppresses MDMX with IC50 of 2.5 μM, leading to enhanced p53 stabilization/activation and DNA damage, and regulates MDM2, an E3 ligase.

Applications Embelin is a natural benzoquinone found in plants of the Myrsinaceae family. Embelin has a wide range of biological activity as it inhibits the growth of bacteria, fungi, protozoa and parasites. Embelin also displays contraceptive and pro-apoptotic properties. References Stasiuk, M. et al.: Glob. J. Biochem., 2, 262 (2011); Brahmeshwari, G. et al.: Adv. Plant Sci., 24, 467 (2011); Hu, R. et al.: Med. Oncol., 28, 1598 (2011);

NF-κB-IN-19 (Compound 8) is an NF-κB inhibitor. It effectively induces DNA damage in tumor cells through the NF-κB signaling pathway and promotes the production of reactive oxygen species (ROS), as well as induces autophagy and apoptosis. Additionally, NF-κB-IN-19 inhibits levels of VEGF and HIF-1α, exerting antiproliferative effects in tumor cells through the PI3K/AKT and STAT-3 pathways. It is also effective in overcoming cisplatin resistance and exhibits antitumor activity.

DDO-8958 is an orally active and selective BET BD1 inhibitor, exhibiting a KD of 5.6 nM for BRD4 BD1. It shows low nanomolar inhibitory activity across all BET BD1 bromodomains except for BRDT BD1. DDO-8958 inhibits tumor cell proliferation and migration, induces apoptosis and cell cycle arrest, and demonstrates antitumor activity.

4-Hydroxyresveratrol (3,4,5,4'-Tetrahydroxystibene) is a resveratrol analog that variably induces the expression of pro-apoptotic genes such as p53 and Bax. It triggers apoptosis in SV40 virus-transformed WI38 cells (WI38VA), but not in WI38 cells. Additionally, 4-Hydroxyresveratrol significantly increases the expression of p53, GADD45, and Bax genes in WI38VA cells, while suppressing the expression of the bcl-2 gene.

Oleuropein is an antioxidant polyphenol isolated from olive leaf.

(-)-Zeylenol (Zeylenol) is a natural product derived from the stems of the Uvaria grandiflora, showing anti-inflammatory, antifeedant and antitumor biological

Sulforaphene, from radish seeds, aids cancer cell apoptosis and inhibits migration; has an ED50 for velvetleaf at ~2x10^-4 M.

Sodium salicylate (2-Hydroxybenzoic acid sodium salt), a metabolite of acetylsalicylic acid, can inhibit NF-kB and reduce oxidative stress.

Licofelone (ML-3000) is a dual COX/LOX inhibitor potentially for the treatment of osteoarthritis.

Benzyl selenocyanate is a chemopreventive agent that effectively inhibits chemically induced tumors at both initiation and postinitiation stages in animal models. It acts as a potent inhibitor of DNA (cytosine-5)-methyltransferase (Mtase) with an IC50 of 8.4 μM.

3-Butylidene-4,5-dihydrophthalide is an effective constituent extracted from Angelica sinensis.

Propiomazine is an orally active antihistamine compound with sedative properties, useful for controlling insomnia in neurological disorders.

Vorinostat (SAHA) is a pan-histone deacetylase (HDAC) inhibitor (IC50=10 nM) with inhibitory activity against HDAC1/2/3/6/7/11.

8-Prenylnaringenin is a phytoestrogen with high estrogenic activity,

Nrf2 activator-11 (compound M11) is a Nrf2 activator that possesses blood-brain barrier permeability and offers anti-oxidation, anti-inflammation, anti-ferroptosis, and anti-apoptosis properties. It is applicable for use in studying cerebral ischemia-reperfusion (CI/R) injury models.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse iNV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse iNV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse iNV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse iNV in the samples is then determined by comparing the OD of the samples to the standard curve.

CHS-828 (GMX1778), a pyridyl cyanoguanidine, is an effective inhibitor of NAD+ biosynthesis enzyme NAMPT (IC50 <25 nM).

nor-NOHA acetate (Nω-Hydroxy-nor-L-arginine acetate) is a reversible inhibitor of arginase, with anti-leukemic activity, effective in endothelial dysfunction,

Wogonin (Vogonin) is a cell-permeable and orally available flavonoid that displays anti-inflammatory and anticancer properties.

Oxybenzone (Eusolex 4360) is an organic compound used in sunscreens. It is a derivative of benzophenone.

Lidocaine HCl: local anesthetic, antiarrhythmic, stronger & longer-lasting than procaine but shorter than bupivacaine/prilocaine.

DPP-21 is an inhibitor of microtubule protein polymerization (IC50: 2.4 μM). It exhibits antiproliferative activity against various cancer cell lines, with IC50 values of 0.38 nM (HCT116), 11.69 nM (B16), 5.37 nM (HeLa), 9.53 nM (MCF7), 8.94 nM (H23), and 9.37 nM (HepG2). DPP-21 induces cell cycle arrest at the G2/M phase of mitosis and subsequently triggers apoptosis in tumor cells by decreasing Bcl-2 while increasing pro-apoptotic protein Bax levels.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APAF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APAF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

LJH685 is an effective pan-RSK inhibitor for RSK1/2/3 (IC50: 6/5/4 nM).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LOX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LOX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LOX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LOX1 in the samples is then determined by comparing the OD of the samples to the standard curve.

EI1 (Ezh2 inhibitor) is a potent and selective EZH2 inhibitor with IC50 of 15 nM and 13 nM for EZH2 (WT) and EZH2 (Y641F), respectively.

Amuvatinib (MP470) is an orally bioavailable synthetic carbothioamide with potential antineoplastic activity.

Metoprolol Tartrate is a blocker of the cardioselective β-adrenergic receptor.

PROTAC 20S proteasome subunit β5 degrader 1 (compound 12f) serves as a targeted degrader of the 20S proteasome subunit β5, exhibiting a DC50 value of 0.11 μM in FaDu cells. This compound disrupts the cell cycle and promotes apoptosis (apoptosis), while inhibiting cell proliferation and migration in both FaDu and KM3/BTZ cells. It is applicable for research into resistance to Bortezomib in pharyngeal carcinoma and multiple myeloma.

Azathioprine (BW 57-322), an immunosuppressive agent, inhibits purine synthesis and GTP-binding protein Rac1 activation.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTbR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTbR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LTbR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTbR in the samples is then determined by comparing the OD of the samples to the standard curve.

D609 (Tricyclodecan-9-yl-Xanthogenate) has antiviral and antiproliferative activities.D609 acts through competitive inhibition of PC, PC-P and SMS.

Polyphyllin G (Polyphyllin VII), the the main member of polyphyllin family, shows strong anticancer activity against several carcinomas.

PFK-158 is an effective and specific inhibitor PFKFB3.

Momordin Ic (Momordin 1c) might represent a potential anticancer activity, by inducing apoptosis through oxidative stress-regulated mitochondrial dysfunction

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFRSF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFRSF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFRSF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFRSF8 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

TrxR/EGFR-IN-1 (Compound L1Au2) is a TrxR/EGFR inhibitor with activity against both gefitinib-sensitive and -resistant lung cancer, effectively suppressing tumor proliferation and promoting apoptosis. It enhances GPX4 degradation through autophagosome-lysosome and proteasome pathways, leading to ferroptosis. Additionally, it induces endoplasmic reticulum stress and triggers immunogenic cell death, making it applicable for studies on gefitinib-resistant lung cancer.

Brusatol (NSC-172924) is a natural product isolated from the Brucea javanica plant. It inhibits Nrf2.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse STAT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse STAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse STAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse STAT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Millepachine is a natural product found in the Chinese herbal medicine with strong antitumor effects against numerous human cancer cells.

PLD-IN-1 (Compound 3r) is an orally effective inhibitor of phospholipase D (phospholipaseD) with an IC50 of 1.97 μM. It reduces the expression of CD24, CD47, and PD-L1 while enhancing the expression of calreticulin, thereby modulating the immune evasion mechanisms of lung cancer cells by promoting the phagocytosis of cancer cells by macrophages. PLD-IN-1 inhibits the viability of lung cancer cell lines A549, HCC44, H460, and HCC15 with IC50 values of 18.44, 22.31, 24.85, and 21.45 μM, respectively. It induces apoptosis (apoptosis) in A549 cells and inhibits cell migration. Additionally, PLD-IN-1 increases pro-inflammatory M1 macrophage levels and decreases anti-inflammatory M2 macrophage levels, exhibiting anti-tumor activity in mouse models.

TREM2-IN-1 (OPA) is a platinum-based TREM2 inhibitor with anti-cancer and anti-tumor activity, inhibiting the immune-regulatory activity of TREM2 on macrophages

Meisoindigo (Natura-α) is a derivative of indigo natural, might induces apoptosis and myeloid differentiation of acute myeloid leukemia (AML).

SNS-032 (BMS-387032) is a selective inhibitor of CDK2 and is 10- and 20-fold selective over CDK1/CDK4. SNS-032 is sensitive to CDK7/9, and no effect on CDK6.

NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively).

Tapotoclax (AMG-176) is an orally active MCL1 inhibitor for the study of myeloid leukemia.

BCP-T.A is an iron death inducer that acts by binding to GPX4.

Applications An Amadori compound having the potential to alter cellular adhesion, inhibit cancer metastasis and induce apoptosis.

9-dihydro-13-acetylbaccatin III (9-DHAB III)is an apoptosis inducer. It shows cytotoxicity against the MCF7 cell line and drug-resistant cell line MCF7-ADR.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is an active metabolite of Melatonin with antioxidant and free radical scavenging activity.

Bcl-xL antagonist 2 is an effective and selective antagonist of Bcl-xL with an IC50 of 91 nM and a Ki of 65 nM.

Vincristine sulfate is a natural alkaloid. Vincristine sulfate is used as a microtubule destabilizer for the study of tumors. Cost-effective, quality assurance.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PKCe in the samples is then determined by comparing the OD of the samples to the standard curve.

AT7519 Hydrochloride (AT7519 HCl) is a multi-CDK inhibitor for CDK1, 2, 4, 6 and 9.

Sinapinic Acid (Synapoic acid) is a phenolic isolated from the roots of Hydnophytum formicarumJack. It is an HDAC inhibitor and also inhibits ACE-I activity.

Tubulin inhibitor 32 is a microtubule inhibitor with antiproliferative and antitumor activity that induces apoptosis and cell cycle arrest in the G2/M phase.

Applications Myristoleic Acid induces apoptosis and necrosis in human prostrate cancer cell LNCaP, and is of a potential for developing an attractive new tool for the treatment of prostrate cancer. References Iguchi, K., et al.: Prostate 47, 59 (2001)

Butein is a cAMP-specific PDE inhibitor, protein tyrosine kinase inhibitor, and SIRT1 activator.Cost-effective and quality-assured.

Batabulin (T138067), an antitumor agent, binds selectively to β-tubulin, halts microtubule formation, and induces apoptosis.

Copanlisib (BAY 80-6946), a PI3K inhibitor, may block tumor growth and improve cancer treatment efficacy.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Bcl2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Bcl2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Bcl2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Tetrahydroxyquinone (Tetroquinone) is a primitive anticataract drug, is also a highly redox active molecule that can take part in a redox cycle.

Genistein (NPI 031L) is a natural soy isoflavone, a multi-targeted tyrosine kinase inhibitor. Genistein has antitumor effects. Cost effective and quality assured.

PKM2 modulator 2 (compound C599) is a potent inhibitor of PKM2, displaying antiproliferative activity and inducing apoptosis. This compound holds potential for research in glioblastoma.