Enzyme

Lipase 32 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 7 and temp. optimum at 50-70°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 32 was shown to hydrolyze p-Nitrophenyl esters of butyrate (100 % activity), octanoate (88 %), laurate (11 %), palmitate (0.3 %) and stearate (0.1 %).

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation

N/A.

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

eXrase DNA endonuclease from enGenes is a recombinant endonuclease from Serratia marcescens produced in E. coli. Effectively and efficiently degrades all forms of DNA and RNA, reducing sample viscosity without proteolytic activity. As effective and efficient as other nucleases on the market, eXrase DNA endonuclease is the most cost-effective way to improve proteins yields and improve sample handing. Presented as a ready to use colourless liquid, formulated in Tris buffer at pH 8.0 with 50 % glycerol (v/v). This tech grade version is our most cost effective endonuclease for R&D applications. eXrase DNA endonuclease is suitable for the effective breakdown of nucleic acids in numerous biotech settings:  • Removal of residual host DNA from biotechnological products to meet regulatory standards • Reduction of viscosity and streamlined purification in downstream processing of fermentation procedures. • Reduction of viscosity in in upstream fermentation processes • Extraction and/or synthesis of flavouring nucleotides • Enhanced bioavailability of nucleotides in specific feed products • DNA degradation for the removal or prevention of biofilm formationeXrase DNA endonuclease from enGenes is made by a proprietary microbial fermentation process utilizing Escherichia coli cells. This enzyme facilitates the hydrolysis of phosphodiester bonds in various forms of DNA and RNA, including single-stranded, double-stranded, linear, circular, or supercoiled configurations, yielding smaller oligonucleotides typically composed of 2-4 base pairs. Unit-Definition: One unit (U) of the enzyme is defined as the amount required to digest calf thymus DNA, yielding acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 within a 30-minute timeframe at pH 8.0 and 37°C. This standardization allows for consistent measurement of enzymatic activity across different batches.

Ribonuclease A (RNase A) is widely used to break down RNA in DNA purification. RNase A catalyzes the endonucleolytic cleavage of phosphodiester bonds of RNA.

PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

SplD Protein, S.

TrkB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

MEK1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

Aconitase catalyzes isomerization of citrate to isocitrate via cis-aconitate. Systemic enzyme name is aconitate hydratase; EC 4.2.1.3.

UBE2K Protein, Human, Recombinant (His & SUMO) is expressed in E.

SET and MYND domain-containing protein 2, also known as HSKM-B, SMYD2, and KMT3C, is a member of the SMYD protein family.

PLOD3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-10xHis tag.

RAB1A Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

N/A.

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt

Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of L-alpha-amino acids.

Carboxypeptidase Y (EC 3.4.16.1) is an exopeptidase enzyme. It hydrolyzes peptide bonds of C-terminal residues and it remains active in the presence of urea at low to moderate concentrations. One unit of the Carboxypeptidase Y will hydrolyze 1.0 μmole of a chromogenic peptide substrate, releasing C-terminal alanine and generating a light-absorbing product.

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin.

Lipase 31 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 6-8 and temp. optimum at 40-80°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 31 was shown to hydrolyze p-Nitrophenyl esters of acetate (100 % activity) and butyrate (23 %).

Endoglycosidase H (Endo H) is a highly specific enzyme that cleaves N-linked oligosaccharides from glycoproteins, targeting high-mannose and certain hybrid-type N-glycans. It does not act on complex-type N-glycans, which are typically processed further in the Golgi apparatus. Due to its specificity, Endo H is widely used in research to study intracellular protein trafficking and post-translational modifications, particularly those occurring during glycoprotein maturation.This product is available as vials for research applications with pre-defined quantities of liquid enzyme solutionSee our EndoH (EEH01.7) available for large-scale bulk supply of liquid enzyme solution for the deglycosylation of proteins in manufacture.For removal of all N-linked carbohydrates from proteins see Cymit Quimica's PNGase F enzymes (PNG01.3 - vials for research and PNG01.7 - large-scale bulk supply)

Polypeptide N-acetylgalactosaminyltransferase 3(GALNT3) belongs to the glycosyltransferase 2 family and galNAc-T subfamily.

UBE2F is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction.

CANT1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Glucose-6-phosphate dehydrogenase (G6PD, G6PDH; EC 1.1.1.49) is an enzyme that catalises the following reaction:  D-glucose 6-phosphate + NADP+ + H2O ⇌ 6-phospho-D-glucono-1,5-lactone + NADPH + H+  One unit of G6PD will oxidize 1.0 µmole of D-glucose 6-phosphate to 6-phospho-D-glucono-1,5-lactone per min in the presence of NAD+ at pH 7.8 and 30 °C. NADP+ is available here.

The aldolase family members involved in metabolism and glycolysis are present in three isoforms: ALDOA, ALDOB, and ALDOC.

PCSK9 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag.

Proto-oncogene tyrosine-protein kinase Fes/Fps, also known as Proto-oncogene c-Fes, Proto-oncogene c-Fps, Feline sarcoma oncogene, FES and FPS, is a protein

Proteinase K, high-quality, liquid, recombinant is an advanced enzymatic product, which is a serine protease derived through recombinant DNA technology. It cleaves peptide bonds to facilitate protein digestion with broad substrate specificity. Its robust proteolytic activity is optimal under various conditions, including a wide range of temperatures and pH levels, making it incredibly versatile. This enzyme is primarily utilized in molecular biology for the preparation of high-quality nucleic acids, ensuring the removal of protein contaminants. It is essential in applications such as DNA and RNA extraction, analysis of protein structure, and cell lysis. Its recombinant nature provides advantages in terms of consistency, purity, and activity compared to non-recombinant counterparts, making it highly reliable for sensitive experiments. It is critically employed in diagnostic assays, genomics research, and biotechnological processes requiring effective proteolysis.

KRAS Protein, Human, Recombinant (G12C, His) is expressed in E.

D1Pas1 Protein, Mouse, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P16381.

Carboxypeptidase B2 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

ABCC1 Protein, Human, Recombinant (His) is expressed in E.

GUCY2C Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag.

ST2/IL-1 RL1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells.

Catalyzes the conversion of malonyl-CoA to acetyl-CoA.

Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into

DHX33 Protein, Mouse, Recombinant (His) is expressed in E. coli with N-6xHis. The accession number is Q80VY9.

Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family.

Nitrile hydratase 17 recombinantly expressed in E. coli comes in a freeze-dried formulation. Nitrile hydratases can be utilized to convert nitriles into their corresponding amides, e.g. to produce acrylamide from acrylonitrile. Additional applications include the removal of nitriles from industrial wastewater. Our nitrile hydratases have been tested for hydrolysis of the following substrates:cyclohexanecarbonitrile, cinnamonitrile, benzonitrile, methacrylonitrile, pivalonitrile.

SVTLE Protein, Protobothrops flavoviridis, Recombinant (His & Myc) is expressed in E.

Tyrosinase-IN-19 (compound 9), a competitive tyrosinase inhibitor, exhibits potent antioxidant activities by neutralizing ROS, ABTS+, and DPPH radicals and

MMP-8 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Phosphodiesterase II from bovine spleen is an enzyme derived from the spleen of cattle, which serves as a crucial biological catalyst for the hydrolysis of phosphodiester bonds in nucleotide sequences. This enzyme's mode of action involves cleaving the phosphodiester linkages within nucleic acids, facilitating the breakdown of these macromolecules into smaller nucleotide units. The primary applications of Phosphodiesterase II extend to biochemical research, particularly in the study of nucleotide metabolism and the understanding of degradation pathways of nucleic acids. Scientists utilize this enzyme to dissect complex biological processes and to model the degradation mechanisms that occur within living organisms. By providing insights into the hydrolytic processes, Phosphodiesterase II is instrumental in advancing research in molecular biology, biochemistry, and related fields. Its specificity and efficiency in breaking down nucleotide chains make it a valuable tool for experimental procedures aimed at elucidating the mechanisms underpinning cellular nucleotide turnover and recycling.

Creatine kinase MB/CKMB Protein, Human, Recombinant is expressed in E. coli.

Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase.

PKN1 Protein, Human, Recombinant (His) is expressed in E.

Probable GTP-binding protein that may be involved in cell development.

B3GNT1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

ATG4B Protein, Mouse, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q8BGE6.

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.

Enterokinase Protein, Bovine, Recombinant (His) is expressed in yeast with His tag.

Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.

UCHL3 Protein, Mouse, Recombinant (His) is expressed in E.

ST2/IL-1 RL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture.

Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily.

Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily.

Matrix metalloproteinase-12(MMP12) is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family.

Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate

Fumarase is an enzyme that catalyze the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid or Krebs cycle.

CES5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

The diazepam binding inhibitor (DBI), alternatively known as the acyl-CoA binding protein (ACBP), is involved in multiple biological actions.

Coagulation factor XII Protein, Human, Recombinant (His) is expressed in E. coli with N-10xHis. The accession number is P00748.

Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the

ZAP70 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P43403.

Aberrant STK24 expression was an independent prognostic indicator in lung adenocarcinoma patients.

METTL11A Protein, Human, Recombinant is expressed in E.

Catalyzes the conversion of L-lactate to pyruvate. Is coupled to the respiratory chain.

Glucosyltransferase (GTase) is the enzyme that transfers glucose to another organic molecule (aglycon), establishing glycosidic linkage. UDP-glucose dependent GTases are part of the enzyme family of glucosyltransferases, they are versatile tools in glucosylation reactions. Different GTases have different substrate specificities. For the specificity of GT201 Glucosyltransferase, please see Table 1 that lists the available GTases with GT201 highlighted.Color: beigeForm: lyophilisateProtein content: 0.5 mg/mgThe glucosyltransferase was tested in a glucosylation reaction of a preferred substrate. High conversion after up to 24 h reaction time was observed. Not sure which GTase to choose? Consider our Glucosyltransferase kit, which contains all 8 Glucosyltransferases.

NLRP3 Protein, Human, Recombinant (His) is expressed in E.

PPM1A Protein, Human, Recombinant (His) is expressed in E.

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular

FDFT1 Protein, Human, Recombinant (His) is expressed in E.

2-Ketoglutaric acid is a key molecule in the tricarboxylic acid cycle,is also connected to glutamic acid and glutamine metabolisms through the transamination

Sulfotransferase family cytosolic 2B member 1, also known as Sulfotransferase 2B1, ST2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, SULT2B1

Hydrolyzes galactooligomers with a degree of polymerization higher than 3.

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids.

TYK2 Protein, Human, Recombinant (E.

PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes.

Citrate synthase Protein, Rickettsia australis, Recombinant (His & Myc) is expressed in E.

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA.

Metalloprotease that is part of the quality control system in the inner membrane of mitochondria.

In a single vial and in a single step, the X-ShiningTM all-in-one ATP Assay provides an optimized reaction solution for the specific bioluminescent detection of ATP.  Simply reconstitute, and the X-ShiningTM all-in-one ATP Assay solution can be directly applied to samples for ATP measurement in a 1:1 ratio for an immediate bioluminescent read-out.  The measurement of ATP levels is crucial to study cell cultures, cell viability, cell response, biochemical processes, to monitor environmental sample activity levels, to assess water quality, to test for biological contamination and to assess biocide efficacy. The X-ShiningTM all-in-one ATP Assay provides sufficient reagent solution to perform 50-to-100 measurements in a 96-well plate and can be easily adapted to measurements in tubes. Find out more about our innovative X-ShiningTM range here X-Shining | Innovation | Cymit Quimica Carbosynth.

HADHB Protein, Mouse, Recombinant (His & Myc) is expressed in E.

GALNS Protein, Human, Recombinant (GST) is expressed in E.

Cell wall formation.

Tyrosine Hydroxylase Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

Please enquire for more information about CalB 02 including the price, delivery time and more detailed product information at the technical inquiry form on this page

Brk Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes.

Catalyzes the reversible phosphorylation of pyruvate and phosphate.

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-

hCAI/II/IV-IN-28(WAY-638358) is a potent carbonic anhydrase inhibitor with potential anticonvulsant activity.

Sedoheptulokinase (SHPK) belongs to the FGGY kinase family, and is mainly located in cytoplasm.

Xanthine oxidase is an enzyme that catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid

Compound 6T, designated as Carbonic anhydrase inhibitor26, acts as an inhibitor of Carbonic Anhydrase II (Carbonic AnhydraseII), exhibiting an IC50 value of 9.10 ± 0.26 μM.

kynureninase/KYNU Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

PLCZ1 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli with N-6xHis-SUMO. The accession number is Q86YW0.

Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine.

PDHB Protein, Human, Recombinant (His) is expressed in E. coli.

Ornithine decarboxylase (ODC1) is an enzyme which belongs to the Orn/Lys/Arg decarboxylase class-II family.

It is a tyrosine kinase inhibitor.

HPGDS/PGD2 Protein, Human, Recombinant (His) is expressed in E.

Flanvotumab (IMC-20D7S) is a humanized antibody targeting TYRP1 with strong antitumor effects via NK cells and ADCC.

Endopeptidase that degrades various components of the extracellular matrix such as collagen.

DDX3X Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus insect cells with N-10xHis and C-Myc tag.

The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-

Hyaluronidases, a family of enzymes that are able to degrade hyaluronic acid (HA), are employed in medicine to increase drug diffusion and reverse the effects

Penase is an enzyme, specifically a type of beta-lactamase, which is derived from bacterial sources. Its mode of action involves the hydrolysis of the beta-lactam ring present in penicillin molecules. This enzymatic activity renders penicillin inactive by breaking its critical structural component that is essential for its antibacterial activity. In research and clinical settings, Penase is utilized to study antibiotic resistance mechanisms and the effects of beta-lactamase production in bacteria. Additionally, it is used in laboratory assays to quantify penicillin or as a control to assess the specific activity of penicillin-targeted treatments. By understanding the interaction between penicillin and Penase, scientists can better comprehend the challenges posed by antibiotic-resistant strains and develop strategies to combat them. Its application is crucial in the field of microbiology, particularly in understanding the dynamics of bacterial adaptation and resistance.

Hyaluronidases, a family of enzymes that are able to degrade hyaluronic acid (HA), are employed in medicine to increase drug diffusion and reverse the effects

HER3/ERBB3 Protein, Rhesus, Recombinant is expressed in HEK293 mammalian cells.

Tyrosine-protein kinase Lyn is a member of the Src family of protein tyrosine kinases, which is mainly expressed in hematopoietic cells, in neural tissues liver

Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation.

Human Deoxyhypusine Synthase (DHS) is vital for the first step of hypusine biosynthesis.

Required for corrinoid utilization.