Neuroscience

Applications (S)-Ketamine-d6 Hydrochloride is labelled (S)-Ketamine (K165310, HCl salt) which is the more active enantiomer of the NMDA receptor antagonist and anesthetic agent Ketamine (K165300). References Meliska, C.J. et al.: J. Pharmacol Exp. Therap., 212, 206 (1980); Smith, D.J. et al.: Neuropharmacology, 20, 391 (1981); Klockgether-Radke, A.P. et al.: Eur. J. Anaesthesiol., 22, 215 (2005)

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP17 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP17 in the samples is then determined by comparing the OD of the samples to the standard curve.

Darenzepine is a muscarinic receptor inhibitor.

Amperozide (FG 5606), an atypical antipsychotic, antagonizes 5-HT2A and modulates dopamine activity for schizophrenia treatment.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse DA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DA in the samples is then determined by comparing the OD of the samples to the standard curve.

Licochalcone B inhibits human bladder cancer cells, reduces inflammation by NO, TNFalpha, and MCP-1, and a derivative protects against endotoxin shock.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ProBDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ProBDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ProBDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ProBDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

ML337 is a negative allosteric modulator of mGlu3 with an IC50 of 593 nM.

(RS)-MCPG ((±)-MCPG) is a non-selective group I/II metabotropic glutamate receptor antagonist.

PPADS tetrasodiuma is a P2X receptor antagonist and an inhibitor of the inverse mode of Na/Ca²⁺ exchange in guinea pig airway smooth muscle.

ALEPH (hydrochloride) acts as a partial agonist of h5-HT2A and h5-HT2B receptors, with EC50 values of 10.3 nM and 19.2 nM, respectively. It can induce head twitch responses in mice, with an ED50 of 0.80 mg/kg.

Baclofen HCL, a GABA-B agonist, treats muscle spasticity by reducing excitatory transmission.

VU0453595 is a M1 positive allosteric modulator (PAM).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CAST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CAST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CAST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CAST in the samples is then determined by comparing the OD of the samples to the standard curve.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human EM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EM2 in the samples is then determined by comparing the OD of the samples to the standard curve.

ETAP, a dual inhibitor of both monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B), exhibits antidepressant-like effects and is utilized in the research

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR2C in the samples is then determined by comparing the OD of the samples to the standard curve.

GSNKGAIIGLM(131602-53-4(free base)) (Amyloid beta-peptide(25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide.

Nitroxazepine (CIBA 2330Go), a tricyclic antidepressant, inhibits serotonin and norepinephrine reuptake, used in depression research.

Dipraglurant: a blood-brain permeable mGluR5 NAM, orally active, selective, IC50 = 21 nM, treats PD-LID.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HPCAL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HPCAL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HPCAL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HPCAL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Anti-Nestin/NES Antibody (9E461) is a Mouse antibody targeting Nestin/NES. Anti-Nestin/NES Antibody (9E461) can be used in ELISA, WB, IHC, IF, FCM.

Paroxetine hydrochloride hemihydrate (Paxil) is an effective and selective serotonin reuptake inhibitor (SSRI).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CHGA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CHGA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CHGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CHGA in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AchE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AchE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AchE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AchE in the samples is then determined by comparing the OD of the samples to the standard curve.

AMPA Receptor Modulator-5 (Example 217) serves as an AMPA receptor modulator, utilized in neurological disease research [1].

Applications The meta isomer of Fluoromethcathinone (F591580), a new substance of abuse. References Osorio-Olivares, M., et al.: Bioorg. Med. Chem., 12, 4055 (2004), Rosner, P., et al.: Forensic Sci. Int., 148, 143 (2005),

BACE1-IN-5 inhibits BACE1 (IC50: 9.1 nM) & Aβ production (IC50: 0.82 nM), enhances hERG inhibition & P-gp efflux.

BRL-15572 dihydrochloride: 5-HT1D antagonist, pKi 7.9, also binds 5-HT1A/2B, 60x selective over 5-HT1B.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP-B in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEFTY1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEFTY1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEFTY1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEFTY1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPt in the samples is then determined by comparing the OD of the samples to the standard curve.

4-Chloromethamphetamine hydrochloride is a novel psychoactive substance belonging to the amphetamine class.

Nefiracetam (DM9384), in Phase 2 trials, enhances GABA, choline, monoamine systems, and treats Ro 5-4864 convulsions.

Gamma-DGG TFA is an antagonist of excitatory amino acids that can inhibit depolarization induced by NMDA, Kainate, and Quisqualate. In addition, it counteracts excitatory postsynaptic potentials (e.p.s.p.) in rat hippocampal slices.

VU6004909 is an mGlu5 receptor orthosteric modulator that reverses MK801-induced increased cortical activity and cognitive dysfunction.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GH in the samples is then determined by comparing the OD of the samples to the standard curve.

4F 4PP oxalate is a 5-HT2A antagonist.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve.

Nitrazolam is a benzodiazepine compound that may exhibit central nervous system depressant properties similar to traditional benzodiazepine drugs by acting on the GABA receptors (GABA receptor). These effects include sedation, hypnosis, anxiolytic, and anticonvulsant activities.

Lintopride HCl is a 5HT-4 antagonist.

Applications Labelled (R)-Modafinil. Used for treatment of excessive sleepiness, α1-adrenoceptor agonist. References Saletu, B., et al.: Int. J. Clin. Pharmacol. Res., 9, 183 (1989), Chemelli, R.M., et al.: Cell, 98, 437 (1999), Purnell, M., et al.: J. Sleep Res., 11, 219 (2002), Dinges, D., et al.: Curr. Med. Res. Opin., 22, 159 (2006)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARRb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARRb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARRb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARRb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

WAY-100635 maleate is a selective 5-hydroxytryptamine 1A receptor antagonist with an IC50 value of 0.91 nM and a Ki value of 0.39 nM.

Sertraline hydrochloride (Sertraline HCl) is a selective serotonin uptake inhibitor that is used in the treatment of depression.

Pyrazolam is a psychoactive benzodiazepine compound serving as a central nervous system depressant. [Pyrazolam] can lead to psychomotor impairment.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPN5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Anti-GPC3 Antibody (2J984) is a Mouse antibody targeting GPC3. Anti-GPC3 Antibody (2J984) can be used in ELISA, WB, FCM.

Ramosetron hydrochloride (YM-060) is a serotonin 5-HT3 receptor antagonist. It is used to treat diarrhea-predominant irritable bowel syndrome and nausea.

BZAD01 is a selective NMDA NR1A/2B receptor antagonist.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NGB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NGB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGB in the samples is then determined by comparing the OD of the samples to the standard curve.

Lu AF21934 is a brain-penetrant and selective mGlu4 receptor positive allosteric modulator (IC50: 500 nM, human).

γ-Aminobutyric acid (4-Aminobutyric acid) is the most common inhibitory neurotransmitter in the central nervous system.

Pempidine (1,2,2,6,6-Pentamethylpiperidine) is a nicotinic antagonist, has been used as a ganglionic blocker in the treatment of hypertension.

Applications Norhydromorphone is a novel metabolite of Hydromorphone (H714650). References Cone, E., et al.: J. Pharmaceut. Sci., 66, 1709 (1977), Konishi, M., et al.: Xenobiotica, 24, 591 (1994), Vaccarino, A., et al.: Brain Res., 666, 104 (1994), Bartlett, S., et al.: Life Sci., 57, 609 (1995),

AMN082 is an mGluR7 agonist with antidepressant effects. Neuroprotective effect of AMN082 on neuronal apoptosis in rats with traumatic brain injury.

Vamicamide (FK 176) is a novel anticholinergic compound. Vamicamide increases spontaneous locomotor activity and inhibits cataleptic convulsions

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-PSA in the samples is then determined by comparing the OD of the samples to the standard curve.

RS 56812 is a 5-HT3 receptor antagonist and agonist used in the study of cognitive disorders.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DAT in the samples is then determined by comparing the OD of the samples to the standard curve.

Eptapirone (F 11440) is a highly effective and selective 5-HT1A receptor agonist with marked antidepressant and anxiolytic potential.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPY in the samples is then determined by comparing the OD of the samples to the standard curve.

Talipexole dihydrochloride (Domnin) is a dopamine D2 receptor agonist, α2-adrenoceptor agonist, and 5-HT3 receptor antagonist in both rat cortical and