Nutrition Research

Applications Preparation from Naringen, a flavanone glycoside occurring naturally in grapefruit. It is used as sweetening agent, especially in chewing gum and dentifrices. References Inglett, et al.: J. Food Sci., 34, 101 (1969), Fisher, J.F., J. Agric. Food Chem., 25, 682 (1977), Pratter, P.J., Perfum. Flavor., 5, 12 (1981), Lina, B.A.R., et al.: Food Chem. Toxicol., 28, 507 (1990),

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.