DNA Cross Linkers

Platinum-based anti-tumor drug

Stability Very Hygroscopic Applications A useful ligand for incorporation of EDTA-metal complexes into proteins particularly for investigating orientation of DNA binding proteins. References Ebright, Y.W., et al.: Biochemistry, 31, 10664 (1992), Flaus, A. and Owen-Hughes, T.: Mol. and Cell. Biology, 23, 21, 7677 (2003), Bruno, M., et al.: Methods Enzymol, 375, 211 (2004)

Mitomycin C is a cytotoxic agent that belongs to the class of mitomycins. It has been used for the treatment of a range of cancers and for ocular surface diseases, such as the glaucoma surgery trabeculectomy. In this case it is administered to reduce post-operative scarring which could otherwise lead to failure of the procedure. Mitomycin C is an alkylating antibiotic that inhibits DNA synthesis by cross-linking the DNA double helix. Mitomycin C also stimulates the production of transforming growth factor β1 (TGF-β1) and basic fibroblast growth factor (bFGF). Clinically, mitomycin can be used both intravenously and topically, and has been studied in a range of wound healing models. Mitomycin C is part of our Bio-X ™  Range. These products are aimed at life science researchers who need high quality ready to use products for assay development, screening or other R&D work. With a solubility datasheet and convenient vials, all of our Bio-X ™  products are in stock across our global warehouses for rapid delivery and ease of use.

Applications An amine reactive trifunctional cross linker.

Cisplatin, also known as cisplatinum, is a platinum-based anti-cancer agent that inhibits the EGFR, which is a receptor tyrosine kinase. It has been shown to have anti-cancer effects in melanoma cells and to inhibit tumor growth in mice. Cisplatin acts by interfering with the mechanism of DNA repairing. It loses one chloride ligand and binds to DNA to inhibit the synthesis, causing DNA damage, and thus inducing apoptosis in cancer cells. In certain studies, the use of cisplatin in combination with other metal chelators has shown improvement in the uptake of the anticancer drug. CisplatiN - Bio-X™ is part of our Bio-X™ Range. These products are aimed at life science researchers who need high quality ready to use products for assay development, screening or other R&D work. With a solubility datasheet and convenient vials, all of our Bio-X™ products are in stock across our global warehouses for rapid delivery and ease of use.

Stability Light Sensitive Applications Used in the synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes. References Laan, W., et al.: ChemBioChem., 11, 1236 (2010), Trammell, S., et al.: Bioconjugate. Chem., 12, 643 (2001),

Applications Labelled Methyl Methanethiosulfonate (M321500). Used for the rapid and selective modification of sulfhydryl groups of enzymes. Useful for mapping the pore-lining regions of the ryanodine receptor. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Stauffer, D.A., and Karlin, A.: Biochemistry, 33, 6840 (1994), Yang, N. et al.: Neuron, 16, 113 (1996), Kuner, T. et al.: Neuron, 17, 343 (1996), Ehrlich, B.E., et al.: J. Gen. Physiol., 109, 255 (1997), Dunten, R.L., et al.: Biochemistry, 32, 3139-3145 (1993)