Others

Butyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring long chain leaving groups.

Aldol 491 acetate is a food and environmental testing chemical, which can be used for the detection of bacteria. Aldol 491 acetate provides a chromogenic substrate for the detection of enzymes and can be used as a substrate in enzyme-linked immunosorbent assay (ELISA) procedures. Aldol 491 acetate also has chemiluminescent properties and can be used as a fluorogenic substrate.

5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-galactosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is used in lactose intolerance screening.

Chromogenic substrate for ?-glucanase

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.

Applications (E)-Isomer of labelled Entacapone polymorphic form A. Peripherally acting inhibitor of catechol-O-methyl transferase (COMT), an enzyme involved in the metabolism of catecholamine neurotransmitters and related drugs. Antiparkinsonian. References Karlsson, M., et al.: J. Pharm. Biomed. Anal., 10, 593 (1992), Nissinen, E., et al.: Arch. Pharmacol., 346, 262 (1992), Wikberg, T., et al.: Eur. J. Drug Metab. Pharmacokinet., 18, 359 (1993),

4-Nitrophenyl 4,6-ethylidene-?-D-maltoheptaoside is a substrate used in a reference measurement method for ?-amylase activity in the diagnosis of acute pancreatitis, approved by the International Federation of Clinical Chemistry (IFCC). The substrate is hydrolysed by serum ?-amylase into shorter 4-nitrophenylated oligosaccharides and their free counterparts. These reaction products are channeled into an indicator reaction with ?-glucosidase and are degraded into glucose and the chromophore, 4-nitrophenol.

5-Bromo-6-chloro-3-indolyl sulfate potassium salt is a fluorogenic substrate that can be used for food testing. It is a conjugate of 5-bromo-6-chloroindolylsulfate and potassium, which is dissolved in buffer solution. This product can also be used as a chromogenic substrate in environmental testing, an enzyme substrate in diagnostics, and a ligand for bioluminescence. It has high purity and quality, and can be used for chemiluminescence or bioluminescence. The CAS number for this product is 6581-24-4.

Chromogenic substrate for ?-galactosidase

Chromogenic substrate targeting alpha-D-galactosidase

5-Nitro-3-indolyl-b-D-galactopyranoside is a substrate for a number of enzymes, including beta-galactosidase, beta-glucuronidase, and beta-mannosidase. It is used as an enzyme substrate in diagnostics to measure the activity of these enzymes. 5N3IG was also used to study the effects of conjugation on enzyme activity. 5N3IG is available in high purity and high quality at CAS No.

Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of ?-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of ?-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.