Others

Modified naphtol AS substrate used in histochemical and cytochemical staining

Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.

This chemical is a ligand that can bind to the enzyme luciferase. In a bioluminescence reaction, this chemical reacts with oxygen and ATP to produce light. This product has been tested for purity and quality and is suitable for use in culture media, environmental testing, or diagnostics. It has also been shown to be an excellent fluorescent substrate for fluorogenic or chromogenic substrates in enzyme substrate assays.

5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.

5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.

3-Indolyl b-D-cellotrioside is a chemiluminescent substrate that is used for the detection of bacterial activity. 3-Indolyl b-D-cellotrioside reacts with hydrogen peroxide to produce light, which can be detected by luminometers. This product can be used in diagnostic tests to detect the presence of bacteria in food, water, and other samples. It has been shown to have excellent performance characteristics and high purity and quality.

Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.

Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining but is less sensitive than other precipitating HRP substrates.

5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).

Stability Hygroscopic Applications Used in the synthesis of cyanogen glycoside. A component of antiperspirants, deodorants, body soaps, shampoos, hair rinses, and hair; it inhibits volatile steroid formation by resident bacteria. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Marouf, A., et al.: Pharm. Biol., 39, 263 (2001), Fukuda, T., et al.: Biol. Pharm. Bull., 26, 271 (2003), Butler, M., et al.: J. Nat. Prod., 67, 2141 (2004), He, J., et al.: J. Nat. Prod., 69, 121 (2006),

Naphthol AS-BI b-D-glucopyranoside is a substrate for enzymes such as β-glucosidase and β-glycosidase. It has been used in diagnostic tests to detect the presence of amylase, lipase, α-amylase, or maltase in urine and feces. In biochemistry, naphthol AS-BI b-D-glucopyranoside is often used as a chromogenic or fluorogenic substrate for the detection of β-galactosidases and other glycosidases. This product has also been used in the detection of bacteria in culture media. Naphthol AS-BI b-D-glucopyranoside is a high quality reagent that can be used for chemiluminescence experiments, food testing, and environmental testing.

4-Nitrophenyl 2-acetamido-3-azido-2,3-dideoxy-b-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. It has been used in the detection of beta-galactosidase activity in culture media, and for the detection of beta-galactosidase activity in environmental samples. 4NPAG can be conjugated to a variety of molecules, including antibodies, proteins, nucleotides, and oligonucleotides. 4NPAG can be used as an enzyme substrate for both chromogenic and fluoroquinolone substrates.