pH Buffer Solutions

Reagecon's Low Ionic Strength pH Buffer Solution at 20°C suitable for accurate measurement of low ionic strength samples. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

20X Borate Buffer is a stock solution that is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at alkaline pH. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Borate Buffer pH remains 8.5, when diluted to 1X with water.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s Antimony pH Buffer Solution is colour coded for ease of use and phosphate free. This pH Buffer Solution is formulated for use in intra-gastric pH analysis using antimony or equivalent electrodes. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 8.00 at 25°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

A ready-to-use 5X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 5X SDS PAGE Sample buffer :1. Tris is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart. 2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge. 3. The high density of glycerol ensures the sample moves down into the well. 4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes. 5. The dye visually indicates the location (tracking) of the sample in the gel.

Reagecon’s Mercury Free pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 2.00 at 20°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's Buffer Standard pH 4.000 at 25°C Certified Reference Material is colour coded for ease of use; produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's Buffer Standard pH 8.00 at 20°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's DIN 19267 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s Borax Free pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

A ready-to-use 2X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 2X SDS PAGE Sample buffer : 1 .Tris-HCl is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart. 2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge. 3. The high density of glycerol ensures the sample moves down into the well. 4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes. 5. The dye visually indicates the location (tracking) of the sample in the gel.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 9.22 at 20°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's Buffer Standard pH 9.40 at 25°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's pH Buffer Solution at 38°C is tested and NIST traceable.

Reagecon’s NIST Value pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 10.012 at 25°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Phenylmethylsulfonyl fluoride (PMSF) is a chemical compound that inhibits the enzyme acetylcholinesterase, which is responsible for the breakdown of acetylcholine. It has been shown to inhibit the production of nitric oxide and superoxide anion by human polymorphonuclear leukocytes. Phenylmethylsulfonyl fluoride also inhibits matrix metalloproteinases in neural cells. The inhibition of these enzymes leads to a reduction in the rate of cell growth and proliferation. In addition, PMSF inhibits signal peptide cleavage by cytoplasmic proteinases, which may be one of its mechanisms for inhibiting protein synthesis. This drug also has an effect on nitrate reductase activity, as well as x-ray diffraction data from biological samples such as rat urine and polymerase chain reaction (PCR) data from DNA samples. PMSF possesses michaelis-menten kinetics with respect to its substrate,

Reagecon's pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 6.00 at 25°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon’s pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's DIN 19266 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s Mercury Free pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

6x Gel Loading Dye, SDS+ is a pre-mixed loading buffer with two tracking dyes for agarose and non-denaturing poylacrylamide gel electrophoresis. The solution contains SDS which results in sharper bands as some restriction enzymes remain bound to DNA following cleavage. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.

Reagecon's Buffer Standard pH 3.00 at 20°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s Technical pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's DIN 19266 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's High Resolution Colour Coded pH Buffer Solution at 25°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's DIN 19267 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 20°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon’s pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol (BNTE) is a nitrilotriacetic acid derivative that is used in biochemical and cell culture experiments. It is a potential biomarker for colorectal adenocarcinoma and can be used to analyze the conformational properties of proteins. BNTE has been shown to have a high chemical stability and can be used as an alternative to sodium citrate in sample preparation. BNTE reacts with nucleophiles such as hydroxyl groups, which are present in proteins or peptides, to form stable tertiary amine intermediates. These intermediates are then reacted with electrophiles such as hydrogen peroxide in the presence of enzymes such as horseradish peroxidase. This reaction mechanism leads to the formation of a mass spectrum characteristic of group P2 atp levels that can be detected by plasma mass spectrometry.

Reagecon’s Mercury Free pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's Buffer Standard pH 10.062 at 20°C Certified Reference Material is produced under our ISO 17034 (A2LA Ref: 6739.01) Accreditation and is NIST traceable. - ISO 17034 accreditation requirements include raw material characterisation; homogeneity and stability studies; assignment of certified values as well as packaging and documentation control.

Reagecon's DIN 19266 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's DIN 19266 pH Buffer Solution at 25°C is tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.

Reagecon's pH Buffer Solution at 20°C is colour coded for ease of use. Tested by our ISO 17025 Accredited Test Method (A2LA Ref: 6739.03) and NIST traceable.