Enzymes

XbaI is an endonuclease enzyme isolated from strains of Xanthomonas badrii, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is TCTAGA, which, as the complementary sequence is AGATCT, has rotational symmetry.

BamHI is an endonuclease enzyme isolated from strains of B.amyloliquefaciens H, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATCC, which, as the complementary sequence is CTTAGG, has rotational symmetry.

QuickRxn HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. In molecular biology it is used as a restriction enzyme of 35.8kDa. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is AAGCTT, which, as the complementary sequence is TTCGAA, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm or CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration: 20U/mcl

DraI is an endonuclease enzyme isolated from strains of Deinococcus radiophilus having the DraI gene, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme.It recognizes the following sequence and produces a blunt end.

HpaI is an endonuclease enzyme isolated from strains of Haemophilus parainfluenzae, recombinant, E. coli and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is GTTACC, which, as the complementary sequence is CAATGG, has rotational symmetry.

NotI is an endonuclease enzyme isolated from strains of Nocardia otitidis-caviarum and is part of the restriction modification system.In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is GCGGCCGC, which, as the complementary sequence is CGCCGGCG, has rotational symmetry.