Enzyme Substrates

4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is a fluorogenic substrate for beta-glucuronidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is commonly used in bacterial detection and for drug metabolism studies as well as lysosomal storage disorder screening.

L-Proline 7-amido-4-methylcoumarin hydrobromide salt is a fluorogenic peptide substrate for proline-specific peptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

Modified naphtol AS substrate used in histochemical and cytochemical staining

Chromogenic substrate for β-D-Cellobiosidase yielding a blue precipitate.

This chemical is a ligand that can bind to the enzyme luciferase. In a bioluminescence reaction, this chemical reacts with oxygen and ATP to produce light. This product has been tested for purity and quality and is suitable for use in culture media, environmental testing, or diagnostics. It has also been shown to be an excellent fluorescent substrate for fluorogenic or chromogenic substrates in enzyme substrate assays.

5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.

5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.

Chromogenic beta-maltosidase substrate

3-Indolyl b-D-cellotrioside is a chemiluminescent substrate that is used for the detection of bacterial activity. 3-Indolyl b-D-cellotrioside reacts with hydrogen peroxide to produce light, which can be detected by luminometers. This product can be used in diagnostic tests to detect the presence of bacteria in food, water, and other samples. It has been shown to have excellent performance characteristics and high purity and quality.

Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.

4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraoside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraosideas a substrate for measuring the chitinase activity is used for fungal infection detection and Gaucher disease screening.

Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining but is less sensitive than other precipitating HRP substrates.

Please enquire for more information about AquaSpark® beta-D-glucoside, 10 mM in DMSO, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191 including the price, delivery time and more detailed product information at the technical inquiry form on this page

Chromogenic substrate for ?-glucuronidase

5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).

Applications Labelled N-Methylnicotinamide, a possible inhibitor of ADP-ribosylation. It is also a classical substrate for renal secretion. References Rankin, P., et al.: J. BIol. Chem., 264, 4312 (1989); Dutt, A., et al.: J. Pharmacol. Exper. Ther., 261, 1222 (1992)

Naphthol AS-BI b-D-glucopyranoside is a substrate for enzymes such as β-glucosidase and β-glycosidase. It has been used in diagnostic tests to detect the presence of amylase, lipase, α-amylase, or maltase in urine and feces. In biochemistry, naphthol AS-BI b-D-glucopyranoside is often used as a chromogenic or fluorogenic substrate for the detection of β-galactosidases and other glycosidases. This product has also been used in the detection of bacteria in culture media. Naphthol AS-BI b-D-glucopyranoside is a high quality reagent that can be used for chemiluminescence experiments, food testing, and environmental testing.