Enzyme Substrates

Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color

Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.

Bioluminescent substrate for cypridina luciferase

Chromogenic substrate for β-D-glucosidase. Yields a red precipitate upon cleavage.

Applications Labelled 3-O-Methyl-L-DOPA (M303815). 3-O-Methyl-L-DOPA is the major metabolite of L-DOPA via catechol O-methyltransferase (COMT). 3-Methoxy-L-tyrosine is neither a substrate for nor an inhibitor of L-amino acid decarboxylase activity. Inhibition of COMT enhances the anti-Parkinson activity of L-DOPA. References Soares-da Silva, P., et al.: Brain Res., 863, 293 (2000), Deleu, D., et al.: Clin. Pharmacokinet., 41, 261 (2002),

Aldol 518 Palmitate is a high purity and high quality, bioluminescent substrate for use in diagnostic applications. Aldol 518 Palmitate is an enzyme substrate that is used for the detection of hydrogen peroxide through chemiluminescence or bioluminescence. It can be used in the diagnosis of bacterial infections and to test food products for spoilage. The enzyme substrate has a CAS number of 646-12-2.

Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.

2-Naphthyl-β-D-galactopyranoside is a substrate for β-galactosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

5-Acetamidofluorescein-di-(b-D-galactopyranoside), also known as C2FDG, is a fluorogenic substrate for beta-galactosidase that generates a green fluorescence after cleavage (Em ~520 nm). It is used in enzyme activity assays and in flow cytometry in animal, bacterial, and yeast cells.

2-Dodecylresorufin is a fluorescent probe that reacts with the hydroxyl radicals in the cell, which are generated by the enzyme superoxide dismutase. 2-Dodecylresorufin can be used to measure reactive oxygen species (ROS) and ROS-induced cellular damage. It is also used to study oxidative stress in vitro. The chemical structure of 2-dodecylresorufin contains a linker arm that provides a reversible linkage to the cell surface, allowing for extracellular measurements of reactive oxygen species (ROS). This compound has been shown to have high reactivity, making it useful for studying population dynamics and fluorescent techniques.

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a useful substrate for enzymatic assays. This high-sensitivity chromogenic pNP substrate offers enhanced selectivity and increased detection ability, allowing for a broad range of applications in the field of glycosidase research. Featuring rapid colorimetric readouts with easily detectable endpoint determination, this substrate delivers clear and accurate results every time.

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)-α-D-galactopyranoside is a chemiluminescent substrate that has been used in the detection of bacterial activity. It is used in the detection of bacterial activity through chemiluminescence and can be conjugated to an enzyme or protein for increased sensitivity. This product has been shown to have high purity and quality and is suitable for use in diagnostic applications.

2-Chloro-4-nitrophenyl beta-D-maltotrioside, often abbreviated as CNP-G3, is a chromogenic substrate to detect and mesaure alpha-amylase activity. Evaluation and monitoring of alpha-amylase activity is important to help diagnose certain diseases such as pancreatitis.

2,4-Dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside, sometimes referred as 2F-DNPG, is an enzyme substrate for measuring beta-glucosidase activity. It is a mechanism-based inactivator of glycosidases, including beta-glucocerebrosidase (Gcase), a lysosomal enzyme encoded by the GBA1 gene responsible for breaking down glucosylceramide into ceramide and glucose. The use of 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside as an enzyme substrate permits the study of the catalytic mechanism and reaction intermediate of these enzymes.

4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-N-acetylgalactosaminidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the diagnosis of Schindler-Kanzaki disease and other lysosomal storage disorders.

2-Naphthyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside (NAG tetra acetate) is a chromogenic enzyme substrate commonly used in the detection of β-galactosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is frequently used in bacterial assays to monitor gene expression or as a tool for screening potential inhibitors of the enzyme.

4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays

L-Leucine-PAB-resorufin is a substrate for enzymes such as β-glucuronidase, α-glucosidase, and β-galactosidase. It is used in the diagnosis of bacterial infections and the detection of certain bacteria in urine and stool samples. L-Leucine-PAB-resorufin can be used to detect the presence of bacteria that produce β-galactosidase and/or β-glucuronidase by releasing resorufin from the substrate. This reaction produces a blue color in the presence of a fluorogenic substrate like fluorescein diacetate or fluorescein isothiocyanate. L-Leucine-PAB-resorufin is also used to detect bacteria that produce α-glucosidase by releasing fluorescamine from the substrate. This reaction produces a green color in the presence of a chrom