Enzyme Substrates

2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside is a compound that is commonly used as an enzyme substrate for glycogen synthase kinase (GSK). It is activated by GSK-3β and has been shown to inhibit the activity of this enzyme. This compound has also been found to have inhibitory effects on other enzymes such as carbonic anhydrase and lactate dehydrogenase. Additionally, 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside exhibits antifungal activity against various fungi, including those resistant to other antifungal agents such as fluopyram and prothioconazole. Its mechanism of action involves interfering with ergosterol biosynthesis, an essential component of fungal cell membranes. Furthermore, this compound has potential applications in the development of new drugs targeting GSK-3β and β-catenin signaling pathways, which are implicated in various diseases including cancer and neuro

4-Nitrophenyl 4-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2,3,6-tri-O-acetyl-b-D-thioglucopyranoside is a superior chromogenic substrate for the detection of enzymes. Specifically designed for optimal performance, it provides exquisite sensitivity and definitive results in enzyme assays. Its exceptional properties make it a top choice among researchers and professionals in biochemistry, enabling accurate analysis and quantification of enzymatic activities.

Bz-Gly-D-Lys-OH is a chemiluminescent substrate used for the detection of peroxidase, catalase, and other oxidoreductases. It is a high purity, high quality product that can be used in a variety of applications. This product has been shown to have an activity of approximately 10% at pH 4.0 and 100% at pH 8.0. Bz-Gly-D-Lys-OH has been tested for its ability to produce chemiluminescence in the presence of hydrogen peroxide and luminol. This product is still undergoing environmental testing and should not be used as a food additive or dietary supplement without further authorization from the FDA.

6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/product

Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.

4-Methylumbelliferyl phosphocholine (4MUPC) is a synthetic phenacyl ester that is used in the study of phospholipid metabolism. It binds to the enzyme phosphodiesterase, which is found in mammalian cells and has been shown to be activated by sodium taurocholate. This compound can be used to detect the presence of this enzyme in cell cultures. 4MUPC binds to a type strain of Clostridium perfringens, allowing it to be identified as a member of this species. The binding affinity is also strong enough for the detection of quinacrine-resistant strains of Clostridium perfringens. 4MUPC also binds to a monoclonal antibody that recognizes phosphatases, which are enzymes that hydrolyze phosphate esters and are involved in lipid metabolism.

L-Glutamic acid gamma-(4-nitroanilide) monohydrochloride is a fluorescent substrate for various enzymes. It is used in the detection of aminopeptidase and dipeptidase activity, as well as in the determination of ATPase activity. L-Glutamic acid gamma-(4-nitroanilide) monohydrochloride is also used in fluorogenic assays for the detection of phosphate, pyruvate, pyrophosphate and sulfite. This product has been tested by both enzyme and cell culture methods to ensure high purity and quality. L-Glutamic acid gamma-(4-nitroanilide) monohydrochloride is not intended for use in diagnostics, food testing or bioluminescence reactions.

4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.

4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranosideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B screening and mucopolysaccharidosis diagnosis.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O (2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-a-D-glucopyranoside is a chemiluminescent substrate that can be used for the detection of aminoglycosides in biological fluids. It is a high purity and high quality product with CAS No. 482743. The product has been tested for safety and purity using culture media and environmental testing. This product may be used as a bioluminescence substrate to detect the presence of aminoglycosides in samples such as urine, serum or plasma. 4NTPAGDG is also useful as a fluorogenic substrate for diagnostics and enzyme substrates for food testing.

Please enquire for more information about Phenytoin sodium including the price, delivery time and more detailed product information at the technical inquiry form on this page

Selenium analogue of firefly luciferin with red-shifted bioluminescence

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

5-Bromo-4-chloro-3-indoxyl choline phosphate is a synthetic enzyme substrate for detecting phosphatidylcholine-specific phospholipase C.

4-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-benzylidene-b-D-glucopyranoside is a fluorescent substrate for beta amylase and other glycosidases. It has been used as a fluorogenic substrate for food testing and as a chromogenic substrate for environmental testing. 4NPB has also been used in diagnostic applications such as enzyme substrates, ligands, and chemiluminescent substrates. This product is of high purity and quality with a conjugate that can be used in biotechnology applications.

4-nitrophenyl 2-acetamido-3-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-3,4,6-tri-O-acetyl -D -galactopyranosyl]-2 deoxy 6 O trityl b D glucopyranoside is a chemiluminescent substrate that can be used in biochemical assays. It reacts with oxygen to produce light when it is excited by a UV or blue light source. This product has been tested for purity and quality by HPLC analysis and spectroscopy.

Chromogenic substrate for esterase with C9 activity yielding a lapis colored precipitate upon cleavage.

4-Methylumbelliferyl beta-D-gentiotrioside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-gentiotriosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease screening.

4-Methylumbelliferyl nonanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl nonanoateas a substrate for measuring the lipase activity is used for the detection of lipase deficiency that could lead to pancreatic insufficiency.

5-Bromo-4-chloro-3-indoxyl myristate is a chemiluminescent substrate that can be used in the detection of peroxide. It reacts with hydrogen peroxide to emit light, which can be detected using a luminometer. 5-Bromo-4-chloro-3-indoxyl myristate is also used for the detection of bacteria, such as Staphylococcus aureus and Escherichia coli, by staining. The compound is also used for diagnostic tests to identify diseases such as tuberculosis. 5-Bromo-4-chloro-3 indoxyl myristate is soluble in organic solvents and water, not toxic, and has a high purity level.