Enzyme Substrates

Please enquire for more information about L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt including the price, delivery time and more detailed product information at the technical inquiry form on this page

Fluorogenic substrate targeting esterases , hGSTP1-1

5-Bromo-6-chloro-3-indoxyl choline phosphate is a chemiluminescent substrate for the detection of bacterial activity. It reacts with an enzyme, luciferase, which is present in a variety of living cells. The reaction produces light. This product has been tested for environmental and food safety by the manufacturer. 5-Bromo-6-chloro-3-indoxyl choline phosphate is used as a diagnostic tool to determine the presence of bacteria in humans, animals, and plants. It can be used to measure levels of bacteria in culture media, water samples, and food products. 5-Bromo-6-chloro-3-indoxyl choline phosphate can also be used as a substrate for enzymes such as phosphatases or esterases that catalyze reactions with substrates with brominated or chlorinated groups.

A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.

Fluorogenic substrate for β-D-Cellobiosidase yielding a blue fluorescent solution upon cleavage.

2-Chloro-4-nitrophenyl b-D-xylobioside is a chemiluminescent substrate that is used as a diagnostic agent. It has been used in the identification of gram positive and gram negative bacteria, which are differentiated by the color of the reaction. This substrate has also been used to detect the presence of certain enzymes produced by bacteria. 2-Chloro-4-nitrophenyl b-D-xylobioside has shown to be an effective enzyme substrate for detecting bacterial growth in culture media and is often used as a fluorogenic substrate in food testing.

Aldol® 515 caprylate hydrochloride is used as a bioluminescent substrate for enzyme activity measurements and as a fluorogenic substrate for detecting C8/C9-esterases, lipase activity yielding a red color.

Luciferin-alpha-D-galactoside, sodium salt is a fluorogenic substrate for use in food testing and environmental testing. It can be used as a chromogenic substrate for the detection of conjugates of bile acids and cholesterol. This product is also used in diagnostic medical tests, such as ligand binding assays, chemiluminescence, and bioluminescence.

Aldol® 495 beta-D-glucuronic acid is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucuronidase activity. The living cells of Escherichia coli can be reliably detected in growth media containing Aldol® 495 beta-D-glucuronic acid, as beta-glucuronidase occurs almost exclusively in E. coli. Aldol® 495 beta-D-glucuronic acid can also be in GUS reporter assays. Aldol® 495 beta-D-glucuronic acid can be used in liquid culture and solid media, and is suitable for use under aerobic and anaerobic conditions. Upon exposure to active beta-glucuronidase, Aldol® 495 beta-D-glucuronic acid develops orange colour and green fluorescence.

5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside is a fluorogenic substrate that can be used to measure the activity of bacterial esterases. The enzyme cleaves the xylose moiety from 5-bromo-4-chloro-3-indoxyl, yielding indoxyl as an intermediate product. This reaction can be catalyzed by esterases found in bacteria, which hydrolyze esters containing a single hydroxyl group at one end and a carboxylic acid or alcohol at the other end. Indoxyl is fluorescent and can be detected using a fluorimeter. 5BX is also chromogenic, so it can be used as an enzyme substrate for detecting beta galactosidase activity in bacteria such as Mycobacterium smegmatis or Escherichia coli. 5BX is also used to detect chemilumines

5-Bromo-4-chloro-3-indolyl b-D-fucopyranoside is a chromogenic substrate used is for the enzyme beta-fucosidase.

Please enquire for more information about BCM® Staphylococcus aureus Plating medium including the price, delivery time and more detailed product information at the technical inquiry form on this page

Please enquire for more information about Aldol® 584 beta-D-glucuronic acid, triethylammonium salt, Cymit Quimica Patent: EP 2427431 and US 8940909 including the price, delivery time and more detailed product information at the technical inquiry form on this page

Aldol® 515 propionate is a chromogenic substrate that is used as a reagent in food testing, diagnostics, and environmental testing. It reacts with an enzyme to form a fluorescent product that can be detected with a spectrophotometer. The substrate has been shown to have high purity and high quality.