Enzyme Substrates

AquaSpark™ β-D-galactoside is a dioxetane-based luminogenic substrate for β-galactosidase activity. β-galactosidase assays are frequently used in molecular biology as reporter gene assays. AquaSpark™beta-D-galactoside can be used to replace X-gal (Cymit Quimica Cat. No.B-7150) that is frequently used in genetically modified bacteria, in the yeast two-hybrid system, or in mouse models to indicate lacZ gene activity. Furthermore, native β-galactosidase activity can be used to detect coliform bacteria, which is important in microbial diagnostics and hygiene monitoring.

Aldol® 355 fluorescence enhancer is an enhancer of fluorescence signals of the Aldol® fluorogenic substrates. Aldol® 355 fluorescence enhancer allows concentrations of fluorogenic Aldol® substrates in media to reduce up to 6 times. Aldol® 355 fluorescence enhancer can be combined with Aldol® substrates, releasing water-insoluble dyes upon enzymatic cleavage, such as Aldol® 470 range, Aldol® 495 range, Aldol® 518 range and Aldol® 515 range of fluorogenic enzyme substrates.

AquaSpark® Enhanced HRP Detection Solution is a sensitive chemiluminescent substrate solution for detecting horseradish peroxidase (HRP) conjugates on immunoblots (Western blot, dot blot) and in enzyme-linked immunosorbent assays (ELISA). The ready-to-use detection solution contains the AquaSpark® substrate and the oxidizing reagent hydrogen peroxide (H2O2).

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium salt is a fluorogenic substrate for beta-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium saltas a substrate for measuring the beta-N-acetylglucosaminidase activity is used for diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB) (a rare lysosomal storage disorder) and Sanfilippo B syndrome screening.

Chromogenic substrate for beta-galactosidase

Jalapeno green-Gal (Jalapeno green-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a Jalapeno green colored precipitate. Jalapeno green-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach.

L-Lysine 7-amido-4-methylcoumarin, acetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

4-Nitrophenyl beta-rutinoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-rutinoside is used in different applications such as the Flavonoid glycoside hydrolysis, food industry applications

Resorufin β-D-galactopyranoside is a non-fluorescent compound and is orange-yellow in color. It is hydrolyzed by the enzyme β-galactosidase (β-Gal) to yield fluorescent resorufin.

Chromogenic cephalosporin substrate for the detection of beta-lactamase activity

AquaSpark® beta-D-apiofuranoside is a chemiluminescent substrate that can be used in the detection of various proteins, such as immunoglobulins, enzymes and hormones. The detection of these proteins can be done by the use of AquaSpark® beta-D-apiofuranoside conjugated with antibodies or enzymes. AquaSpark® beta-D-apiofuranoside is a substrate for the luciferase enzyme and it produces light when activated by a luciferin. This product has been tested for purity, quality and activity in various culture media. The product is also free from any detectable impurities and has passed all environmental testing requirements.

4-Nitrophenyl 2-acetamido-2-deoxy-3,6-di-O-benzoyl-b-D-galactopyranoside is an advanced pNP-based enzyme substrate designed for the detection and measurement of glycosidase activity in biological samples. This synthetic compound offers high specificity and sensitivity for b-galactosidase enzymes, enabling accurate determination of enzymatic hydrolysis rates in a variety of research applications, including diagnostics, drug development, and discovery of novel enzymes. Its chromogenic properties ensure easy analysis of the results and make it suitable for high-throughput screening.

4-Methylumbelliferyl-beta-D-ribofuranoside is a chemical compound that belongs to the class of phenols. It has been found in Australian plants and animals. 4-Methylumbelliferyl-beta-D-ribofuranoside is used as a corrosion inhibitor. This chemical has been shown to be effective against corrosion in mammals.br>br> 4-Methylumbelliferyl-beta-D-ribofuranoside can also be used as a fluorescent probe for studying cell membrane activity.

L-Citrulline-p-nitroanilide hydrobromide is a substrate for enzymes such as beta-galactosidase, beta-glucuronidase, and alkaline phosphatase. It is used in biochemical assays for the detection of microorganisms and in medical diagnostics. This compound has been shown to be useful in fluorescence microscopy and bioluminescence. It also has applications in food testing, environmental testing, and high purity research.

Applications Methyl DL-Mandelate is a substrate for lipase B from Candida antarctica. References Santos, J.C.S., et. al.: Enzyme Microb. Tech., 77, 1 (2015)

Chromogenic substrate for α-mannosidase yielding a salmon colored precipitate upon cleavage.

Please enquire for more information about 6-Chloro-3-indolyl phosphate disodium salt including the price, delivery time and more detailed product information at the technical inquiry form on this page

4-Methylumbelliferyl alpha-D-mannopyranoside (4-MU-a-D-Man) is a fluorogenic substrate used in activity assays for alpha-mannosidase. When it is hydrolyzed by alpha-mannosidase, it produces a fluorescent compound, 4-methylumbelliferone (4-MU). 4-MU-a-D-Man is often used in medical research to monitor the activity of alpha-mannosidase, which is important in lysosomal storage diseases.  In addition, 4-Methylumbelliferyl alpha-D-mannopyranoside has been used to study the the activity of concanavalin A, a lectin that specifically binds to alpha-D-mannopyranosyl and alpha-D-glucopyranosyl residues on various glycoproteins. The use of this fluorogenic enzyme substrate permits study the degree of alpha-D-mannosidic linkage cleavage by the enzyme.

Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.

Esculetin-7-beta-D-galactopyranoside, also known as 6,7-Dihydroxycoumaryl-7-beta-D-galactopyranoside, is a fluorogenic substrate used in the detection of beta-galactosidase activity. This coumarin derivative, when hydrolyzed, yields esculetin (6,7-dihydroxycoumarin) which, in the presence of iron salts, forms a brown-black complex.

6-Chloro-3-indoxyl butyrate is a chemiluminescent substrate that is used in diagnostics and research. It has been shown to be an excellent ligand for the detection of DNA and protein. 6-Chloro-3-indoxyl butyrate can also be used as a chromogenic substrate for the detection of alkaline phosphatase, acid phosphatase, or beta-galactosidase. This product is also used in food testing, enzyme substrates, and fluorogenic substrates.

Chromogenic sulfatase substrate

4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is an advanced chromogenic pNP enzyme substrate that delivers unparalleled performance in enzymatic assays. This high-quality substrate is specifically designed for studying glycosidases and other enzymes involved in carbohydrate metabolism. Our 4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is synthesized with the highest quality materials to provide incredible accuracy and reliability in your research applications.

2-Chloro-4-nitrophenyl a-D-glucopyranoside is an an enzyme-activated irreversible inhibitor of alpha-glucosidase. The substrate 2-Chloro-4-nitrophenyl a-D-glucopyranoside is covalently attached to the enzyme enabling mechanistic studies of glycosidase activity, especificallyt in carbohydrate metabolism studies.

Fluorescent reagent for the detection of phospholipids. Previously under code EA179480.

4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is a chromogenic substrate for alpha-mannosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is used in different applications such as the Lysosomal storage disorder diagnosis (e.g., alpha-mannosidosis)

Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in ?-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

Coelenterazine F is an analog of the natural compound coelenterazine (a luciferin). It is a bioluminescence molecule commonly used as a calcium indicator in cells and tissues.

4-Methylumbelliferyl 2-azido-2-deoxy-4,6-O-phenylmethylene-a-D-galactopyranoside is a fluorogenic substrate that reacts with beta D galactosidase to produce light. This product is useful for diagnostics, conjugate production, and other applications. 4MUF 2 -Gal provides a convenient means of detecting beta D galactosidase activity in cell culture media and can be used as a chromogenic substrate for the detection of enzymes involved in glycosylation reactions. It is also used to detect staining by bioluminescence. The compound can be used in food testing and environmental testing to measure the presence of beta D galactosidase.

Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride (Gly-Gly-AMC) is a fluorescent compound that can be used as a probe for the transfer of glycine in the brain. Gly-Gly-AMC binds to Langerhans cells and is transferred by an anterograde axonal transport mechanism from the peripheral nervous system to the central nervous system. The binding of Gly-Gly-AMC to Langerhans cells has been shown to activate them, which induces the release of inflammatory mediators into the surrounding tissue. This activity may be due to increased levels of calcium ions, which are known to induce exocytosis in these cells. Glycine is also involved in depression and other mental disorders, as well as muscle and brain activity.

The enzyme alkaline phosphatase hydrolyses 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) substrate to 5-bromo-4-chloro-3-indole that turns into a blue coloration upon oxidation.

Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.

Modified naphtol AS substrate used in histochemical and cytochemical staining

Chromogenic substrate of ?-D-glucosidase

4-Nitrophenyl 3,4,6-tri-O-acetyl-b-D-galactopyranoside is a nucleic acid molecule that has been synthesized by recombinant expression in Corynebacterium glutamicum. It is a photosynthetic molecule and can be used to study the molecular basis of photosynthesis. The 4-nitrophenyl group on the sugar moiety confers resistance to degradation by nucleases. 4NP3,4,6GalAc has been shown to be capable of binding to proteins and polypeptides that are important for photosynthesis. This compound also has marginalizing effects on C. glutamicum strains that are used as expression vectors for recombinant protein production.

Z-Gly-Pro-Arg-pnaacetate is a substrate commonly used in enzyme research, particularly in the study of proteases. Proteases are enzymes responsible for catalyzing the hydrolysis of peptide bonds in proteins. In a study investigating the activation of protein C in blood plasma by a protease from Aspergillus ochraceus, the enzyme activity was qualitatively assessed using chromogenic peptide substrates, such as pGlu-Pro-Arg-pNA.

Substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions. AquaSpark® Calibration Probe is used for: Demonstration experiment susing only the probe and an aqueous solution or water; Evaluation of the AquaSpark® maximum light emission; Functional tests of luminometers, plate readers and cameras; Comparison with other probes bearing an enzyme labile group in order to build concentration/response curves; Validation and comparison between different AquaSpark® probes.

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.

N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a high quality and highly purified substrate for enzymes that catalyze the release of light when they react with their specific substrates. It is used in a variety of applications, including diagnostics and food testing. NACBZLAMC hydrochloride is an excellent ligand for chemiluminescence reactions and can be used to detect the presence of various analytes. It can also be used as a staining agent to detect proteins on Western blots or to stain bacterial cultures for identification. This product has been shown to be effective against numerous bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa, as well as fungi like Candida albicans.

This Clips-O substrate 7-(4'-L-Alaninamido)-rac-(3'-octanoyloxy)butyloxy) coumarin allows the detection of three enzymatic activities (L-alanine aminopeptidase, C8-esterase and oxidation of 4-amino-3-hydroxybutyloxy either enzymatically or by exposure to sodium periodate) with one single substrate and give a positive fluorescent signal only in presence of all activities. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.

4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a synthetic compound with the ability to inhibit the enzyme glutamicum. It is a radionuclide that can be used as a focusing agent for gamma cameras and has been shown to be effective against corynebacterium glutamicum and other organisms. 4NP2AG was synthesized by reacting nitrophenol with acetamide in the presence of an acid catalyst and hydrogenating the product. The synthesis employs precession chemistry, which involves rotating a molecule around its axis at high speed to increase its concentration in one area. This treatment leads to a higher yield of desired product. 4NP2AG contains two acetamido groups on the glucopyranoside moiety, which are esterified with nitrophenol and acetic acid respectively. These moieties are

Fluorogenic substrate for β-D-galactosidase yielding a blue fluorescent solution upon cleavage.