Enzyme Substrates

4-Aminoantipyrine has been used as an analgesic and anti-inflammatory agent. It can also target peroxidase enzymes to produce a red color

2-(2-Thiazolylazo)-4-cresyl]phosphate, p-toluidine salt is a chromogenic substrate for alkaline phosphatase resulting in a dark red color.

This product is a chromogenic substrate that is used in the detection of alkaline phosphatase and other enzymes. It is also used in food testing, staining, and diagnostics. This product has high purity with a CAS number of 125328-84-9.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. It can be used in diagnostic tests to identify antibodies against certain bacteria and fungi. The fluorogenic form of 4-methylumbelliferyl 2-acetamido-3,4,6-triO acetyl -2 deoxy a D glucopyranoside can be used as a chemiluminescent substrate for detecting bacterial activity. 4 methylumbelliferyl 2 acetamido 3,4,6 triO acetyl -2 deoxy a D glucopyranoside is also useful in bioluminescent assays to detect ATP production in bacteria and other microorganisms. CAS No: 1174287-76-3

Water soluble substrate for firefly luciferase. Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring. Endotoxin free product.

3-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that is used for the detection of glycosidases. It has been shown to be a good ligand for the study of nucleic acid hybridization, and it can also be used in enzyme assays. 3NPPAG is available in high purity and high quality. This product has been tested for use in culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, CAS No. 61894-63-1, diagnostics, chromogenic substrate, enzyme substrate, food testing and conjugate applications.

4-Methylumbelliferyl 2-O-(a-L-fucopyranosyl)-b-D-galactopyranoside is an enzyme substrate used in chromogenic assays of alpha-fucosidases. It is also used for glycoprocessing enzymes in synthesis.

Chromogenic substrate for alkaline and acid phosphatase

4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-b-D-galactopyranosyl-b-D-glucopyranoside is a chemiluminescent substrate that is used in diagnostic tests. This compound has been approved by the FDA and EPA for use in food testing, as well as for the detection of certain bacteria, parasites, and fungi. It is also used to detect protein kinase activity in cells or tissue samples. 4MUG can be conjugated to an enzyme or antibody to produce a fluorescent signal when activated by light at 350 nm. The compound has been shown to be nonfluorescent when in the dark, but will emit fluorescence upon activation with light at 350 nm.

Luc-phosphate Na is a synthetic drug that is used in the treatment of cancer. It is an inhibitor of DNA methyltransferase, which is a key enzyme in DNA methylation. The inhibition of this enzyme leads to decreased DNA methylation and increased expression of tumor suppressor genes. Luc-phosphate Na also has anti-cancer activity by inhibiting the growth of cells and by inducing apoptosis. This drug has been shown to have no adverse effects on cellular physiology, such as cell division or protein synthesis.

5-Bromo-4-chloro-3-indolyl nonanoate is a fluorogenic substrate that is used in the detection of enzymes. The substrate reacts with various enzymes and produces a fluorescent product, which can be detected using a fluorimeter or luminometer. It has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase. This compound may also be used as an enzyme substrate for the detection of bacteria in culture media or for food testing. 5-Bromo-4-chloro-3 indolyl nonanoate is not mutagenic or genotoxic when tested on bacterial cells.

Caspases are a family of protease enzymes playing essential roles in programmed cell death and inflammation. The acronym caspase stands for Cysteine Aspartic Acid Specific Protease due to their specific cleavage of an aspartic acid residue, by mean of a cysteine in the catalytic site of the enzyme._x000D_About 11 or 12 different caspases are known in humans and 10 in mice, participating in numerous different cellular functions. Caspase deficiency, for example, has been identified as a cause of tumor development, while over-activation of some caspases such as caspase-3 can lead to excessive apoptosis that can be observed in several neurodegenerative diseases. Caspases cleave proteins at specific peptide recognition sequences. Ac-DEVD-6-Amino-D-Luciferin (Ac-Asp-Glu-Val-Asp-6-Amino-D-Luciferin, Cat.No_x000D_L-8282) is a synthetic chemiluminescent peptide for the determination of caspase-3 and caspase-7activity.

This Clips-O substrate 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin allows the detection of single and dual enzymatic activity (L-alanine aminopetidase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.

Please enquire for more information about L-Pyroglutamic acid 7-amido-4-methylcoumarin including the price, delivery time and more detailed product information at the technical inquiry form on this page

5-Bromo-6-chloro-3-indoxyl butyrate is an enzyme substrate that is used for the detection of bacteria and fungi in environmental testing, food testing, and diagnostics. It is also used as a fluorogenic substrate in bioluminescence and chemiluminescence assays. 5-Bromo-6-chloro-3-indoxyl butyrate binds to proteins on the cell surface, causing an increase in fluorescence when excited by light of a specific wavelength. The conjugate can be detected using a variety of methods, including staining with dyes such as safranin or methylene blue. This product has high purity and high quality.

6-Chloro-3-indolyl phosphate disodium salt is a substrate for visualising alkaline phosphatase activity. It produces a red colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

5-Bromo-4-chloro-3-indoxyl-alpha-D-xylopyranoside is an enzyme substrate that can be used in the detection of β-galactosidase activity. This product has been shown to be a good ligand for various xanthine oxidoreductases, including xanthine dehydrogenase and uricase. 5BBIX is also a chromogenic substrate for horseradish peroxidase and can be used to detect hydrogen peroxide in the presence of osmium tetroxide or potassium permanganate. 5BBIX has been extensively tested for use in diagnostics, food, and environmental testing.

Fluorescent dye substrate for flow cytometric measurements

Neu5Ac(a2-6)Gal(b1-3)GlcNAc-b-pNP is a fluorogenic substrate that can be used for testing the presence of glycoproteins in food samples. It also has been used to detect the presence of enzymes in culture media. Neu5Ac(a2-6)Gal(b1-3)GlcNAc-b-pNP is a high purity product that has a CAS number and is available as a conjugate. This product can be purchased in bulk quantities, with a purity of >90%.

Chromogenic substrate for beta-galactofuranosidase

5-Bromo-6-chloro-3-indoxyl butyrate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lipid digestion assays.

6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase, which hydrolyzes the substrate to produce a fluorescent product. This fluorogenic substrate can be used in chemiluminescent detection of β-galactosidase activity as well as in immunochemical and immunohistochemical analysis of glycoproteins. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-galactopyranoside is also used for staining of glycoprotein substrates with periodic acid Schiff reagent or Alcian blue, and it can be used in the diagnosis of glycogen storage diseases.

5-Bromo-4-chloro-3-indolyl acetate (5BCIA) is an enzyme substrate that has been shown to inhibit the activity of a number of enzymes, such as proteases and phosphatases. It has also been shown to inhibit the growth of a number of human pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. 5BCIA inhibits bacterial growth by binding to the active site of the enzyme phosphatase and preventing it from hydrolyzing a phosphate group from ATP. This process is necessary for energy metabolism in all living cells. 5BCIA has been shown to be effective against methicillin resistant Staphylococcus aureus (MRSA).

Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.

4-Nitrophenyl 2-acetamido-3-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic substrate designed for the detection and quantification of enzyme activity. This tetra-acetylated compound is specifically designed to be cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically, allowing for accurate and sensitive analysis of enzyme kinetics and inhibition studies.

Chromogenic substrate for β-D-glucosidase, yielding a blue precipitate and contained in a chromogenic culture media for the detection of Enterococci, Streptococci, Klebsiella species, Enterobacter species and Listeria species. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of the enzyme, beta-glucosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole; this immediately dimerises to give an intensely blue product.

Chromogenic substrate targeting cellulase

4-Nitrophenyl-alpha-D-glucopyranoside, beta-Anomer is a chromogenic sugar that can be used as an indicator for the presence of 4-nitrophenol. It is a highly reactive compound that spontaneously reacts with nitrobenzene to form a dinitroaniline. This anomeric structure is easily oxidized by air and light to form the corresponding o-dinitroaniline. When this reaction occurs, this substance changes color from yellow to red.

4-Methylumbelliferyl elaidate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl elaidateas a substrate for measuring the lipase activity is used for assessment of lipase activity to identify pancreatitis.

5-Dodecanoylaminofluorescein di-b-D-galactopyranoside, commonly known as C12FDG, is a fluorogenic substrate for β-galactosidase. Fluorescein is released upon enzymatic cleavage by β-galactosidase. The intensity of its fluorescence is utilised to quantify β-galactosidase activity. C12FDG has been shown to be useful in identifying senescent cells that are associated with aging and cancer. It has also been used as a model system in some studies for studying disease activity, intracellular targets, and biological sample storage. It is also used to identify cancer tissues and radiation damage in the form of senescent cells. The chemical structure of 5DAFG is similar to that of the natural sugar galactose. 5DAFG binds to the plasma membrane of cells due to its lipophilic properties and then enters the cell through transport proteins on the cell surface called ATP binding cassette transporters.

6-Nitro-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with horseradish peroxidase to produce light. 6NIGP is used in the diagnostic field as a fluorogenic or chromogenic substrate for enzymes such as alkaline phosphatase, and as a staining agent for cells, tissues, and other biological materials. 6NIGP has been used in the detection of bacteria, fungi, parasites, and viruses. It is also used in food testing as an enzyme substrate. 6NIGP is purified by recrystallization from water or acetone and can be stored at −20°C.

[2-Methoxy-4-(2-nitrovinyl)phenyl] b-D-galactopyranoside (MNVP-gal) is a chromogenic enzyme substrate that is commonly used to detect the activity of beta-galactosidase in biological samples. MNVP-gal produces a distinctive magenta color when hydrolyzed by beta-galactosidase, making it particularly useful for applications where visual detection is necessary. This substrate is also resistant to endogenous proteases, making it well-suited for use in cellular and tissue preparations.

1-Naphthyl β-D-mannopyranoside is a chromogenic (also fluorogenic) substrate. After cleavage, 1-naphthol is released and is used to quantify beta-mannosidase activity.

Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color

Resorufin benzyl ether is a chemical compound that can be used as a substrate for cytochrome P450. It has been shown to inhibit the activity of epoxide hydrolase, an enzyme involved in the metabolism of endoperoxides. Resorufin benzyl ether has also been found to inhibit cytochrome P450 enzymes, which are responsible for oxidative metabolism of xenobiotics. This compound has been shown to have a high affinity for mouse monoclonal antibody and is used in immunoassays to detect mouse antibodies. Resorufin benzyl ether is used in biochemical assays and polymerase chain reactions (PCRs) as a fluorescence marker. In addition, this compound is used in model systems to study enzyme activities such as recombinant cytochrome p450 and protein oxidation by reactive oxygen species (ROS).

Beta-Alanine 7-amido-4-methylcoumarin trifluoroacetate is a fluorogenic substrate for aminopeptidase yielding a blue fluorescent solution.

Please enquire for more information about cis-6,7-Dimethyl-5,6,7,8-tetrahydropterin hydrochloride including the price, delivery time and more detailed product information at the technical inquiry form on this page

Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.

4-Nitrophenyl 4,6-O-benzylidene-3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a high purity fluorescent reagent that can be used as a substrate for many enzymes. It is also used in environmental testing and to detect the presence of nitrophenols in food. This product has CAS number 545414.