Enzyme Substrates

Chromogenic substrate for cellobiohydrolase and endoglucanase.

3-Indoxyl phosphate p-toluidine salt is a secretor of blood group A. It is used in the detection of the presence of alkaline phosphatase in serum and other body fluids. This compound has been shown to be an isoform of 3-indoxyl phosphate with a molecular mass of about 4,000 daltons. It is also used as a substrate for gel electrophoresis and filtration experiments. The purified form of this compound has been shown to have an alkaline phosphatase activity that is ˜4 times greater than that found in human serum and intestinal extracts.

4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.

4-Methylumbelliferyl-a-D-mannopyranoside-2-phosphoethanolamine is a fluorescent substrate used in enzyme assays. It is an analog of the natural substrate, 4-methylumbelliferyl-alpha-D-mannopyranoside, which is a phosphoethanolamine linked to alpha-D-mannose. This product can be used as a chromogenic or fluorogenic substrate for various enzymes, including beta glucosidase, beta galactosidase, and beta lactonase. 4MUMPE can also be used for bioluminescent studies and has been shown to have high purity and quality.

Naphthol AS-D is a diagnostic chemical for phosphatase enzymes and is used to stain cells, tissues, and body fluids for diagnosis. The chemical reacts with the amino groups of proteins in cells and produces a brown color. Naphthol AS-D is not toxic to living cells in concentrations below 5%. It stains basophilic leukemia cells as well as brain cells. This chemical can be used to diagnose cutaneous lesions, such as psoriasis or melanoma, which are difficult to diagnose by standard methods.  present in these lesions and produce a brown color.

Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.

5-Bromo-3-indoxyl-beta-D-galactopyranoside-6-sulfate sodium salt is an enzyme substrate that has been used in diagnostic tests for detecting the presence of alpha galactosidase. 5-Bromo-3-indoxylbDgalactopyranoside 6 sulfate sodium salt is a ligand that has been shown to bind to the receptor on macrophages and neutrophils, which are cells that protect the body from infection. 5Bromo3indoxylbDgalactopyranoside 6 sulfate sodium salt can be used as a chromogenic substrate or fluorogenic substrate for chemiluminescence reactions. It can also be used in culture media for bacterial growth and for food testing.

Coelenterazine e is a synthetic, fluorescent amino acid that can be used to measure calcium ion concentration. It binds to calcium ions and emits light when excited with blue light. Coelenterazine e is an analog of coelenteramide, which is a luminescent protein found in jellyfish. Coelenterazine e has been shown to emit fluorescence when it interacts with calcium ions, while coelenteramide emits bioluminescence instead. The emission of light from coelenterazine e is due to the stabilization of the molecule by calcium ions.

Used for synthesis of chiral carboxylic acids from nitriles

6,8-Difluoro-4-methylumbelliferyl b-D-galactopyranoside, commonly known as DiFMUG, is a fluorogenic substrate to detect beta-galactosidase. It has higher sensitivity than the common MUG (4-methylumbelliferyl beta-D-galactopyranoside) subtrate.

Aldol® 518 acetate is a fluorogenic substrate for the enzyme aldolase. It is used in diagnostic kits for detecting aldolase in blood and other body fluids, and as an indicator of liver function. Aldol® 518 acetate can be used to detect the presence of aldolase in foods and beverages. The compound has also been shown to be an excellent substrate for bioluminescence and chemiluminescence reactions, which are widely used in research laboratories.

A fluorogenic MOSCERDAM® substrate targeting cellulases

Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.

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Luciferin-caprylate is a chemiluminescent substrate that is used in the detection of various substances. It is a high purity compound with CAS No. 583-22-8 and an E number of E711. This product can be used in diagnostic, food testing, and other applications. Luciferin-caprylate has been shown to be an excellent substrate for bioluminescence, fluorescence, and chemiluminescence reactions. It generates light through the oxidation of luciferin by the enzyme luciferase in the presence of oxygen or other oxidizing agents. The light generated by this reaction can be detected using a photomultiplier tube or luminometer, which can then be used to detect substances such as bacteria, protozoa, or fungi.

Chromogenic substrate for ?-glucosidase

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.

6-Chloro-1H-indol-3-yl b-D-mannopyranoside is an enzyme substrate that is used in the fluorogenic and chromogenic assays for detecting indoleamines. This compound has a conjugate, which is a fluorescent molecule that can be attached to an antibody to target specific proteins or cells. 6-Chloro-1H-indol-3-yl b-D-mannopyranoside is used as a diagnostic agent in the detection of bacterial and fungal infections. It is also used as an indicator of cell growth in culture media.

Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside can be used to inhibit the growth of bacteria, yeast, and fungi. It is a yellow powder that has been shown to cause regression in tumor xenografts in mice. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside inhibits cellular proliferation by blocking the synthesis of proteins and nucleic acids. This compound also has been shown to have an effect on the environment because it inhibits the growth of phytoplankton. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside was found to disrupt bacterial gene expression profiles, which may be due to its ability to inhibit protein synthesis at the ribosome level.

2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a synthetic substrate for various enzymes, including the enzymes that catalyze the conversion of D-glucose to glucose 6 phosphate. This compound is used as a growth factor and has been shown to inhibit chronic kidney disease in rats. In addition, it has also been shown to regulate matrix metalloproteinase activity in human urine.

1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.

6-Chloro-3-indolyl b-D-cellobioside is a chromogenic substrate that produces a salmon-pink color after enzymatic cleavage. It is used for detecting the activity of beta-D-cellobiosidase in enzyme assays.

3-Indoxyl sulfate potassium salt (3ISKP) is a microbial metabolite that has been shown to have an optimum concentration in the range of 0.5-2.0 mM. It is water soluble, and can be found in human serum and human feces. 3ISKP is synthesized from tryptophan by gut bacteria as well as from dietary sources such as soybean protein. 3ISKP has been shown to inhibit mitochondrial membrane potential, which may lead to apoptosis of cells or inhibition of physiological function. 3ISKP also inhibits fatty acid synthesis, which may affect the metabolism of other nutrients. The analytical method for 3ISKP is LC-MS/MS with a reaction solution containing methanol and acetonitrile in a ratio of 1:1 with a pH range of 4-5.

2-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to detect β-galactosidase activity. This specific substrate is one of the most commonly used substrates for detecting β-galactosidase activity in diagnostic assays. It reacts with the enzyme to produce a blue color and has a fluorescence emission maximum at about 480 nm. The product is available in high purity and high quality and it is also an enzyme substrate.

Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is a glycosylation product of the complex carbohydrate GlcNAc. It is synthesized by the enzyme β-1,3-galactosyltransferase and N-acetylneuraminic acid transferase. This product has been modified using click chemistry to introduce a fluorine atom at the 6 position of Neu5Acα2-6. Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is available in high purity and custom synthesis.

Chemiluminescent substrate for b-galactosidase

Applications Met-enkephalin analog found in opioid extracts of brain and adrenal chromaffin cells that is an artifact generated by trichloroacetic acid extraction. MUS is used as a fluorimetric substrate for arylsulphatase enzyme. Subsitution of the hydroxyl group of 4-methylumbelliferone (4-methyl-7-hydroxy-coumarin) causes the excitation wavelength of the umbelliferone to shift to shorter values and also brings about a decrease in fluorescence.Fluorescence: max. Abs = 334 mm; max. Em. = 370 mm References Harinath, B., et al.: J. Neurochem., 18, 245 (1971), Marniemi, J., et al.: Int. J. Biochem., 4, 95 (1973), Bojarova, P., et al.: ChemBioChem., 9, 613 (2008),

Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their β-galactosidase activity.

5-Bromo-6-chloro-3-indolyl b-D-cellobiopyranoside is a fluorogenic substrate for the enzyme β-glucuronidase. It is used in diagnostic applications such as food testing, bacterial culture media and environmental testing. The product has CAS number: 123591-77-0.

Chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a blue precipitate. Contained in certain mycological culture media. Used in histochemistry.

2-Amino-5-pyridyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-galactopyranose is a chromogenic substrate for the enzyme β galactosidase. It is used to detect the presence of β galactosidase in bacterial cultures. The substrate is soluble in water and has a long shelf life. 2APAAG can be used for food testing and environmental testing as it produces a bioluminescent signal when cleaved by β galactosidase.

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.

Resorufin b-D-glucuronide sodium salt is used as a fluorogenic substrate for beta-glucuronidase (GUS) to study drug metabolism.

4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is an extracellular nucleic acid molecule that is produced by the bacterium Corynebacterium glutamicum. This compound has been shown to inhibit cancer cells by blocking the transport of glucose through the cell membrane and may be a potential drug target for cancer treatment. 4NPTAPG has also been shown to have diagnostic properties and can identify corynebacterium glutamicum strains. It is a fluorescent molecule that can be used to detect the presence of this bacterium in culture media.

Please enquire for more information about 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

2-Aminophthalylhydrazido 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that is used as a diagnostic in biological research. It reacts with enzymes to form fluorescent products, and can be used to detect the presence of bacteria and fungi. This product has a CAS number of 128879-80-1 and can be used for food testing, environmental testing, or bioluminescence. It is an important component in the development of new antibiotics.

[2-Methoxy-4-(2-nitrovinyl)phenyl] beta-D-galactopyranoside is a chromogenic substrate (505 nm) for β-glucosidase.

Disodium salt trihydrate

A MOSCERDAM® substrate targeting the fucosidase enzyme

Fluorogenic substrate for beta-glucosidase yielding a blue fluorescent solution. Used for the detection of beta-glucosidase as an indicator of Enterococci.

5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-galactopyranoside is a fluorescent marker that is used to measure the enzymatic activity of corynebacterium glutamicum. The enzyme catalyzes the conversion of 4-methylumbelliferyl glucopyranoside to 4-methylumbelliferone glucuronide. The production of 4 methylumbelliferone glucuronide can be quantified using fluorescence spectroscopy. This product can be used in research involving chronic inflammatory diseases and cancer.