Enzyme Substrates

5-Bromo-6-chloro-3-indolyl-2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate that is used as an indicator in diagnostic tests. This compound reacts with an oxidizing agent, such as hydrogen peroxide, to generate light. It is also used as a chromogenic substrate for the detection of bioluminescence and it can be applied in culture media or food testing. 5Bromo-6Chloro-3Indolyl2Acetamido2DeoxyA1DGlucopyranoside has a CAS number, which means that it meets the standards set by the Chemical Abstracts Service. It also has a high purity and quality, making it suitable for use in laboratories.

N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride is a fluorogenic substrate that can be used for the detection of β-glucuronidase. It has been shown to have high purity and quality, which makes it an excellent choice for use in food testing and staining.

Green fluorescent cationic dye

D-Ile-Pro-Arg p-nitroanilide dihydrochloride is a chromogenic substrate commonly used in research to assess the activity of various enzymes. It has been utilized in studies related to thrombin inhibition, enzyme assays of protease enzymes, activation and enzymatic activity analysis of Factor VII-activating protease, and as a substrate for measuring glandular kallikrein activity. This substrate has been employed in assays to study the amidolytic activity of enzymes such as thrombin, plasma kallikrein, plasmin, and other proteases.

Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.

Luc-Enterobacteria Na is a bioluminescent substrate for bacterial luciferase used to detect Enterobacteriaceae. The enzyme luciferase oxidizes luciferin with FMNH₂ and O₂ via luxAB genes, emitting blue light.

4-Methylumbelliferyl-β-D-glucuronic acid methyl ester is a fluorogenic substrate that can be used as a ligand for enzyme activity detection. The substrate reacts with an oxidizing agent to produce the fluorescent product 4-methylumbelliferone, which can then be detected with a sensitive fluorometer. 4-Methylumbelliferyl-β-D-glucuronic acid methyl ester is an alternative to the more commonly used fluorescein in enzyme assays. This product has been shown to be an effective chemiluminescent substrate for the detection of bacteria and fungi in culture media, as well as for food testing.

Aldol® 515 nonanoate hydrochloride is a chromogenic and fluorogenic enzyme substrate for detection of lipase activity. The colorless product is cleaved by C9 esterases produced by bacteria in liquid or solid media, yielding a red color and red fluorescence when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 nonanoate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 24-48 h. In the presence of a purified lipase, color and fluorescence develops within minutes to few hours.

Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for ?-glucosidase activity (EC 3.2.1.20, ?-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.

5-Bromo-6-chloro-3-indolyl caprylate (or magenta caprylate) is a chromogenic substrate which can be used for the detection of esterase enzyme activity from Salmonella and some Klebsiella and Enterobacter. The esterase cleaves the ester bond in the substrate, releasing 5-bromo-6-chloro-1H-indol-3-ol which undergoes oxidative dimerization to form 5,5'-dibromo-6,6'-dicholoro-indigo, a magenta coloured chromophore (λmax 565nm). This product is patent pending, as polymorphs of this product can have different physicochemical properties and can improve solubility and stability. Cymit Quimica is able to product high quality, stable product to detect esterase enzyme activity.

Chromogenic substrate for beta-glucuronidase

Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.

4-Methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside is a fluorogenic substrate for alpha-N-acetylgalactosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranosideas a substrate for measuring the alpha-N-acetylgalactosaminidase activity is used for Fabry disease diagnosis and Schindler disease screening.

A substrate used for the diagnosis of Sanfilippo disease C (MPS III C).

BIOSYNTH Culture Medium for the isolation andidentification of Escherichia coli O157:H7

Luciferin-beta-D-galactoside is an acceptor in immunoassays. It is used as a label for specific antibodies and antigens, and the light emitted by this compound can be measured with a luminometer. Luciferin-beta-D-galactoside binds to the bacterial enzyme β-galactosidase, which catalyzes the hydrolysis of lactose to form glucose and galactose. Mammalian cells have been used as a source of β-galactosidase in order to measure luciferin-beta-D-galactoside activity. The substrate specificity of this enzyme has also been studied using mammalian tissue extracts.

5-Bromo-4-chloro-3-indolyl myristate is a chromogenic substrate used to detect the specific enzymatic activity of lipase or esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl myristate is used in screening assays for pancreatic lipase deficiency and lipid metabolism studies.

Applications 6-Hexadecanoylamino-4-methylumbelliferyl phosphorylcholine is a phosphorylcholine derivative and was shown to be a specific substrate for the determination of acid (lysosomal) sphingomyelinase. References Van Diggelen, O.P., et al.: J. Inherit. Dis., 28, 733 (2005);

The 4-Nitrophenyl 2,3,4-tri-O-acetyl-6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is a fluorogenic substrate for β-galactosidase. This product has high purity and quality and is an enzyme substrate for β-galactosidase. The 4NPG2,3,4TRA can be used as a diagnostic tool in the diagnosis of bacterial infections. It is also used in the food testing industry and environmental testing industry. This product has CAS No.: 51743-71-1. This product is also a ligand that reacts with the enzyme β-galactosidase to produce light in chemiluminescence reactions. This product can be used for staining or bioluminescence applications.

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Please enquire for more information about 5-Bromo-6-chloro-3-indoxyl choline phosphate including the price, delivery time and more detailed product information at the technical inquiry form on this page

4-Fluoro-2-nitrophenyl beta-D-galactopyranoside, a fluorinated analog of ONPG, is a chromogenic enzyme substrate specifically used to detect beta-galactosidase in bacterial media. 4-Fluoro-2-nitrophenyl beta-D-galactopyranoside is used in Lactose intolerance screening, bacterial beta-galactosidase detection (E. coli)

4-Nitrophenyl 6-O-(b-D-galactopyranosyl)-b-D-galactopyranoside is a fluorescent substrate that is used in diagnostics and culture media. It can be used to detect the presence of aminoglycoside antibiotics, such as gentamicin, by using the fluorogenic or chromogenic reaction. 4NP6BG has been shown to bind to the beta subunit of RNA polymerase and inhibit transcription, which can be useful for testing environmental samples for the presence of aminoglycosides. 4NP6BG is a high quality product with a CAS number of 80321-98-8.

Fast Blue BB is commonly used for assaying beta-glycosidase activity and the chromogenic reaction between Fast Blue BB and beta-naphthol results in a reddish colored product and is at the basis of its application to different enzymatic activity assays. Produces a fluorescent, blue-green chromophore.

5-Bromo-6-chloro-1H-indol-3-yl-a-D-mannopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lysosomal storage disorder screening.

2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.

5-Bromo-4-chloro-3-indolyl myo-inositol-1-phosphate ammonium is a fluorogenic substrate used in the detection of phosphatidylinositol phosphate kinases. It can be used as a chromogenic substrate for detecting phosphatidylinositol phosphate kinase activity in tissues, cells, and biological fluids. 5-Bromo-4-chloro-3-indolyl myo-inositol 1 phosphate ammonium is also used as a ligand to detect phosphatidylinositol phosphate kinase activity in vivo. This product is of very high purity and quality. 5-Bromo 4 chloro 3 indolyl myo inositol 1 phosphate ammonium can be used in Enzyme Assays, Diagnostics and Cultures Media.

Aldol® 518 beta-D-cellobioside is a chromogenic substrate for the detection of glycosidases. Aldol® 518 beta-D-cellobioside reacts with enzymes such as alpha-amylase, beta-galactosidase, and alpha-glucosidase to produce a fluorescent product. This product is detected by spectrophotometer, fluorometer, or luminometer. The fluorescence intensity is proportional to the concentration of the enzyme in the sample. It has been shown that Aldol® 518 beta-D-cellobioside can be used as a substitute for conventional substrates in food testing and diagnostics, such as staining and culture media. It also has applications in environmental testing and enzyme substrate assays.

X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli ) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing beta;-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta;-D-galactopyranoside (X-beta;-D-galactoside, X-Gal) is a chromogenic indicator for beta;-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate. X-Gal can also be used as a detecting agent for beta;-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta;-galactosidase, color develops within minutes to few hours.

A fluorogenic MOSCERDAM® substrate targeting cellulase

8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.

Luc-Listeria Na is a conjugated substrate for the detection of Listeria monocytogenes in food, environmental and clinical samples. It is a high purity product that can be used for the detection of Listeria monocytogenes from culture media, bioluminescence, chromogenic substrate, and fluorogenic substrate. Luc-Listeria Na is also a ligand for ELISA and IHC assays.

Substrate detecting beta-glucosidase activity

Dehydroluciferin is a bioluminescence probe that is a potent inhibitor of the firefly luciferase reaction. It is a byproduct of the oxidation of luciferin, the substrate for firefly luciferase, which catalyzes the bioluminescence reaction. It can also form during the synthesis or storage of luciferin. Its presence can potentially lead to issues in bioluminescent assays.

Chromogenic substrate for α-D-glucosidase yielding a blue precipitate. It is used for the detection of, e.g. Staphylococcusaureus and Bacillus species, and for the isolation of Enterobactersakazakii e.g. from baby food. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. The enzyme, beta-glucosidase cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.

L-Proline 7-amido-4-methylcoumarin hydrobromide is a fluorogenic substrate that is used to measure esterase and peptidase activity in a variety of animal and microbial sources. This compound has been shown to have a lipase activity. L-Proline 7-amido-4-methylcoumarin hydrobromide is also used as an enzyme preparation and as an enzyme source.

Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

For detection of beta-galactosidase activity.  The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.

Substrate for hydrolases

AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions. AquaSpark® Calibration Probe is used for: Demonstration experiment susing only the probe and an aqueous solution or water; Evaluation of the AquaSpark® maximum light emission; Functional tests of luminometers, plate readers and cameras; Comparison with other probes bearing an enzyme labile group in order to build concentration/response curves; Validation and comparison between different AquaSpark® probes.