Chromogenic Substrates

Please enquire for more information about 5-Bromo-4-chloro-1-methyl-3-indolyl-β-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

This product is a fluorogenic substrate that reacts with the enzyme beta-galactosidase to produce a fluorescent product. It can be used in food testing, environmental testing, and diagnostics. The CAS number for this product is 609-33-2. This product is available in high purity and high quality.

3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.

2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.

6-Chloro-3-indoxyl-beta-D-glucuronic acid (cyclohexylammonium salt) is a chromogenic substrate for beta-glucuronidase yielding a salmon colored precipitate upon cleavage.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.

Chromogenic substrate used to measure phospholipase C (PLC) activity

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3-O-(2,3,4,6-tetra -O acetyl -b D galactopyranosyl)-2 deoxy a D galactopyranoside is a high purity fluorogenic substrate for the enzyme β galactosidase. It can be used for the detection of β galactosidase in biological samples. 4NPAG can also be used as a ligand for various types of chromogenic substrates. This product is also commonly used in diagnostics and conjugates to detect β galactosidase activity or as a culture media supplement.

4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is a chromogenic substrate for glucose-6-phosphatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is used in different applications such as the Glycogen storage disease diagnosis (e.g., von Gierke disease)

Modified naphtol AS substrate used in histochemical and cytochemical staining

Please enquire for more information about 3-Indoxyl myo-inositol-1-phosphate, ammonium salt including the price, delivery time and more detailed product information at the technical inquiry form on this page

Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

5-Bromo-3-indolyl nonanoate (BIN) is a chromogenic enzyme substrate often used for the detection of esterase activity. It is hydrolyzed by esterase to produce a blue product, which can be detected visually or by spectrophotometry. BIN is particularly useful for the detection of esterase activity in bacteria and fungi, and can be used to differentiate between different types of organisms based on their esterase activity patterns

6-Chloro-3-indoxyl-beta-D-glucuronic acid (cyclohexylammonium salt) is a chromogenic substrate for beta-glucuronidase yielding a salmon colored precipitate upon cleavage.

Substrate for beta-N-acetylglucosaminidase

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a state-of-the-art chromogenic substrate designed for rapid and sensitive enzyme activity detection. As a versatile pNP substrate, it produces a noticeable color change upon enzyme-catalyzed hydrolysis, facilitating easy monitoring and quantification of enzyme levels in research and diagnostic applications. The substrate's acetylated glucose moiety ensures specificity and stability, allowing for precise and reliable results across a range of experimental conditions.

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.