Chromogenic Substrates

3-Indolyl b-D-glucuronide sodium salt is a specialized enzyme substrate that features an indole moiety linked to a glucuronide group. This compound is commonly used in various biochemical assays to study the activity of glucuronidase enzymes, which play a crucial role in the metabolism and detoxification of xenobiotics and endogenous compounds. The sodium salt form of this substrate ensures enhanced solubility and stability in aqueous solutions, making it a valuable tool for researchers investigating the function and regulation of glucuronidase enzymes in biological systems. 3-Indolyl b-D-glucuronide sodium salt is also used for the detection of E. coli.

A chromogenic substrate for β-galactosidase.

Chromogenic α-mannosidase substrate yielding a absorbent solution upon cleavage. Also used for studies of the crystal structure of the complexes of concanavalin A and binding studies with concanavalin A.

2-Chloro-4-nitrophenyl phosphate is a chromogenic substrate for phosphatases. Afer cleavage of the substrate, 2-chloro-4-nitrophenol is released, which can be quantified at 405 nm. 2-Chloro-4-nitrophenyl phosphate is used in clinical diagnostics to for prostate cancer, diagnosis of liver and bone diseases.

4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics

2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is a chromogenic substrate used to detect the enzymatic activity of xylosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is used in bacterial hemicellulose metabolism assays.

Modified naphtol AS substrate used in histochemical and cytochemical staining

Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.

This chemical is a ligand that can bind to the enzyme luciferase. In a bioluminescence reaction, this chemical reacts with oxygen and ATP to produce light. This product has been tested for purity and quality and is suitable for use in culture media, environmental testing, or diagnostics. It has also been shown to be an excellent fluorescent substrate for fluorogenic or chromogenic substrates in enzyme substrate assays.

5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.

5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.

3-Indolyl b-D-cellotrioside is a chemiluminescent substrate that is used for the detection of bacterial activity. 3-Indolyl b-D-cellotrioside reacts with hydrogen peroxide to produce light, which can be detected by luminometers. This product can be used in diagnostic tests to detect the presence of bacteria in food, water, and other samples. It has been shown to have excellent performance characteristics and high purity and quality.

Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.

Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining but is less sensitive than other precipitating HRP substrates.

5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).

Naphthol AS-BI b-D-glucopyranoside is a substrate for enzymes such as β-glucosidase and β-glycosidase. It has been used in diagnostic tests to detect the presence of amylase, lipase, α-amylase, or maltase in urine and feces. In biochemistry, naphthol AS-BI b-D-glucopyranoside is often used as a chromogenic or fluorogenic substrate for the detection of β-galactosidases and other glycosidases. This product has also been used in the detection of bacteria in culture media. Naphthol AS-BI b-D-glucopyranoside is a high quality reagent that can be used for chemiluminescence experiments, food testing, and environmental testing.

4-Nitrophenyl 2-acetamido-3-azido-2,3-dideoxy-b-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. It has been used in the detection of beta-galactosidase activity in culture media, and for the detection of beta-galactosidase activity in environmental samples. 4NPAG can be conjugated to a variety of molecules, including antibodies, proteins, nucleotides, and oligonucleotides. 4NPAG can be used as an enzyme substrate for both chromogenic and fluoroquinolone substrates.

4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) diagnosis

2-Methyl-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used in various applications. It is a ligand that binds to the enzyme beta-galactosidase and is used in diagnostics for detecting the presence of the enzyme. 2-Methyl-4-nitrophenyl b-D-galactopyranoside has been shown to be an appropriate substrate for chemiluminescence reactions, such as those used in diagnostic or research applications. The product also has been tested for potential toxicity and found not to be toxic at concentrations up to 100mg/mL. This product can be used in a variety of applications, including food testing, bacterial culture media, and environmental testing.

Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced

The 4-Nitrophenyl a-D-xylopyranoside is a highly sensitive and reliable chromogenic substrate tailored for the detection and analysis of alpha-D-xylosidase enzyme activity. Upon enzymatic hydrolysis, it forms 4-nitrophenol, a yellow compound with remarkable fluorescence that enables a straightforward, quantitative assay in areas including environmental science, metabolism studies, and enzyme modifier exploration.

Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a pink enzyme substrate commonly used in biochemical research and diagnostic applications. This compound is a derivative of indolyl glucopyranoside, which is known for its ability to produce a colored product upon enzymatic hydrolysis. 6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is particularly useful for studying glycosidases, enzymes that cleave glycosidic bonds in complex carbohydrates. Its pink coloration makes it an ideal choice for colorimetric assays, enabling researchers to monitor enzyme activity in real-time and facilitating the development of new diagnostic tools and therapeutic strategies.

Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

Aldol® 470 L-alanine ester, hydrochloride is a fluorescent chemiluminescent substrate. It is a ligand with high purity and high quality, which has been patented in the European Patent Office (EP 2427431) and the United States Patent and Trademark Office (US 8940909). The product can be used for chemiluminescence, staining, fluorescence, bioluminescence, enzyme substrate and food testing. Aldol® 470 L-alanine ester, hydrochloride is an excellent fluorescent chemiluminescent substrate that can be used for conjugation to enzymes or antibodies.

Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a Fluorogenic substrate used for the detection of an enzyme that converts sialic acid to fluorescein. This product has a high quality and purity with a CAS number. It is used in Culture media as a conjugate, Enzyme substrate, Food testing, High purity, Diagnostics, ligand, Chromogenic substrate, Environmental testing. The product has been tested and found to be safe for use with animals and humans.

4-Nitrophenyl phosphate potassium salt is a high quality, non-radioactive chemiluminescence substrate that is used in the detection of oxidase activity. This product can be used to measure the activity of peroxidase and catalase enzymes, as well as the accumulation of hydrogen peroxide. It also has been shown to be a ligand for some sulfhydryl groups and an inhibitor of phosphatases. 4-Nitrophenyl phosphate potassium salt has been tested for its ability to inhibit bacterial growth and fluorescence by staining cells. This product has also been shown to have bioluminescent properties and can be used for diagnostics or culture media.

2,4-Dinitrophenyl 2-deoxy-2-fluoro-b-D-xylopyranoside (DNFX) is a transglycosylation reagent that can be used to inactivate enzymes that catalyze the synthesis of xylosides. DNFX undergoes a covalent reaction with these enzymes and inhibits them by binding to the active site. DNFX catalyses the reaction between xylose and nucleophiles such as hydroxide ions, thiols, or amines. DNFX also has a catalytic effect on electrospray ionization and can be used for the detection of nucleophile activity.

For the detection of beta-galactosidase activity

4-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-2-deoxy-b-D-glucopyranoside is a versatile chromogenic pNP enzyme substrate designed to offer superior results for detecting enzyme activity in research and diagnostic applications. This substrate enables faster, more accurate, and reliable detection of hydrolase enzymes, making it ideal for various research and testing needs, such as studying enzyme kinetics, screening inhibitors, and profiling enzyme specificity.

Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indolyl palmitate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl palmitate is used in lipase activity assays or cystic fibrosis diagnostics.

4-Nitrophenyl 2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that contains a reactive group of 2,3,4-tri-O-levulinoyl. This compound can be used to measure the activity of enzymes such as beta-glucosidase and alpha-galactosidase. 4NP2OMP is an excellent ligand for metal ions and has been shown to bind to copper in the presence of ATP. 4NP2OMP can also be used as a diagnostic agent for measuring the activity of bacterial beta-glucosidase and alpha galactosidase.

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

Chromogenic substrate for beta-D-galactosidase yielding a brown precipitate upon cleavage.

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside is a superior chromogenic pNP enzyme substrate designed to deliver enhanced enzyme detection and outstanding assay performance. This cutting-edge substrate boasts excellent specificity, sensitivity, and stability, making it a powerful tool in the measurement of enzyme activity, substrate profiles, and inhibitor characterization. This premium substrate is perfect for researchers and professionals seeking reliable and efficient results for their enzyme-based investigations.

Aldol 470 acetate is a chromogenic substrate for carboxylesterases yielding a yellow precipitate. Furthermore it shows some fluorescence(excitation at 320 nm to 490 nm). In contrast to classical indoxylsubstrates, Aldol substrates work not only under aerobic but also under anaerobic conditions.

2-Nitrophenyl 2-acetamido-2-deoxy-a-D-galactopyranoside is a fluorogenic substrate that is used in enzyme assays. It can be used as a chromogenic substrate or conjugate with an enzyme to detect the presence of glucose.

4-Nitrophenyl 2-O-(a-L-fucopyranosyl)-a-D-galactopyranoside is a chromogenic pNP enzyme substrate consisting of a fucose and galactose moieties. Upon enzymatic hydrolysis by specific fucosidases or galactosidases, it releases the highly chromogenic molecule 4-nitrophenol, which exhibits a distinct yellow color that can be monitored spectrophotometrically. This substrate is frequently used to study enzyme specificity, kinetics, and inhibition, and it plays a critical role in the investigation of carbohydrate active enzymes (CAZymes), the screening of enzyme inhibitors, and the development of novel analytical methods for the detection and quantitation of enzyme activities.

4-Nitrophenyl-N-acetyl-α-D-glucosaminide is a chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a yellow solution upon cleavage.

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-6)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate used in the detection of sialidase activity. It is also used as a chromogenic substrate in the determination of β -galactosidase activity.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol substrates work not only under aerobic but also under anaerobic conditions

4-Nitrophenyl a-D-maltopentaoside serves as a substrate for enzymes functioning in carbohydrate metabolism, including α-amylase and maltase. Upon enzymatic hydrolysis, it yields a yellow 4-nitrophenolate product that can be easily detected and monitored spectrophotometrically. 4-Nitrophenyl a-D-maltopentaoside . This substrate is ideal for research purposes in fields such as biochemistry, enzymology, and glycobiology, providing an efficient tool for characterizing maltopentaoside-related enzymes and understanding their mechanisms of action.

Chromogenic substrate for alkaline phosphatase

Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3,6-di-O -acetyl2 deoxy b D glucopyranosid e is a chemiluminescence substrate that reacts with hydrogen peroxide to produce light. It is used in diagnostics for the detection of bacterial and fungal cultures. The reaction between 4NPAA and hydrogen peroxide generates light at 540 nm. This product has been tested for purity and quality using various methods including culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, enzyme substrate, food testing and conjugate.

Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color

Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.

Aldol 518 Palmitate is a high purity and high quality, bioluminescent substrate for use in diagnostic applications. Aldol 518 Palmitate is an enzyme substrate that is used for the detection of hydrogen peroxide through chemiluminescence or bioluminescence. It can be used in the diagnosis of bacterial infections and to test food products for spoilage. The enzyme substrate has a CAS number of 646-12-2.

Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.

2-Naphthyl-β-D-galactopyranoside is a substrate for β-galactosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a useful substrate for enzymatic assays. This high-sensitivity chromogenic pNP substrate offers enhanced selectivity and increased detection ability, allowing for a broad range of applications in the field of glycosidase research. Featuring rapid colorimetric readouts with easily detectable endpoint determination, this substrate delivers clear and accurate results every time.

2-Chloro-4-nitrophenyl beta-D-maltotrioside, often abbreviated as CNP-G3, is a chromogenic substrate to detect and mesaure alpha-amylase activity. Evaluation and monitoring of alpha-amylase activity is important to help diagnose certain diseases such as pancreatitis.

2,4-Dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside, sometimes referred as 2F-DNPG, is an enzyme substrate for measuring beta-glucosidase activity. It is a mechanism-based inactivator of glycosidases, including beta-glucocerebrosidase (Gcase), a lysosomal enzyme encoded by the GBA1 gene responsible for breaking down glucosylceramide into ceramide and glucose. The use of 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside as an enzyme substrate permits the study of the catalytic mechanism and reaction intermediate of these enzymes.

Chromogenic β-maltosidase substrate yielding a yellow solution upon cleavage.

4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-N-acetylgalactosaminidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the diagnosis of Schindler-Kanzaki disease and other lysosomal storage disorders.

4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays

Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Chromogenic substrate for esterase with C9 activity yielding a salmon colored precipitate upon cleavage.

6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.

Histochemical substrate for alkaline phosphatase

Please enquire for more information about N-(4-Nitrophenyl)-D-glucopyranosylamine including the price, delivery time and more detailed product information at the technical inquiry form on this page

Chromogenic substrate for beta-glucosidase

Chromogenic substrate for β-glucuronidase (GUS) yieldinga blue precipitate. It is used for detection of GUS+ bacterial colonies and has been reported as chromogenic substrate in a number of chromogenic E. coli / coliform culture media. About 95% of common E. coli possess β-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products, shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli β-glucuronidase) gene fusion marker constructs in plants.

Please enquire for more information about 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide including the price, delivery time and more detailed product information at the technical inquiry form on this page

4-Nitrophenyl 2-O-(2,3,4,6-tri-O-acetyl-a-D-mannopyranosyl)-4,6-O-benzylidene-a-D-mannopyranoside is a fluorogenic substrate that is used for the detection of alpha amylase activity in food testing. It is also used as a chromogenic substrate for the detection of beta amylase activity. 4NP2ODM is a conjugate that can be used for the detection of alpha or beta amylases in environmental testing. This compound has high purity and quality and CAS No. 90105037.

4-Nitrophenyl 4-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2,3,6-tri-O-acetyl-b-D-thioglucopyranoside is a superior chromogenic substrate for the detection of enzymes. Specifically designed for optimal performance, it provides exquisite sensitivity and definitive results in enzyme assays. Its exceptional properties make it a top choice among researchers and professionals in biochemistry, enabling accurate analysis and quantification of enzymatic activities.

Chromogenic substrate for alpha-glucosidase

Phosphoric acid, mono(4-nitrophenyl) ester is an inhibitor that binds to the human serum albumin (HSA), thereby preventing it from binding to the transcriptional regulatory protein. This leads to a decrease in the activity of this protein and consequently less inhibition of gene expression. The structural analysis of this inhibitor has shown that it forms hydrogen bonding interactions with basic proteins, while its reactivity is mainly dependent on Michaelis-Menten kinetics. The inhibitor also inhibits enzyme activities by binding to their active sites, reducing the enzyme's ability to catalyze reactions.

Aldol 470 phosphate is a chromogenic substrate for phosphatases yielding a yellow precipitate. Furthermore it shows some fluorescence (excitationat 320 nm to 490 nm). In contrast to classical indoxyl substrates, Aldolsubstrates work not only under aerobic but also under anaerobic conditions.

8-Hydroxyquinoline-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used for enzymatic detection of peroxidase. The substrate is available in a variety of forms, including powder and liquid. This product has been tested for environmental and bioluminescence activity, as well as food safety. It has also been shown to be a useful ligand in immunoassays.

3-Indoxyl phosphate, p-toluidine salt is a fluorogenic substrate that can be used for the detection of β-galactosidase. 3-Indoxyl phosphate, p-toluidine salt is a highly active chromogenic substrate that can be used as a diagnostic tool in the food industry to determine whether or not milk has been adulterated with animal milk. This product is also used in environmental testing to detect bacteria and fungi. 3-Indoxyl phosphate, p-toluidine salt is a high purity, high quality conjugate that can be used for the detection of β-galactosidase.

4-Nitrophenyl-beta-L-fucopyranoside is a chromogenic substrate used in the determination of alpha-glucosidase activity. The formation of phenol from 4-nitrophenyl leads to the production of a yellow color that can be quantified by measuring its absorbance at 490 nm. This assay is based on the principle that alpha-glucosidase hydrolyzes sucrose into glucose and fructose, which are then oxidized to form 4-nitrophenol and 4-nitroanomeric acid, respectively. The reaction product is detected by measuring the absorption at 490 nm.

Chromogenic substrate for neuraminidase yielding a blue precipitate upon cleavage.

4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) screening

5-Bromo-4-chloro-3-indoxyl choline phosphate is a synthetic enzyme substrate for detecting phosphatidylcholine-specific phospholipase C.

4-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-benzylidene-b-D-glucopyranoside is a fluorescent substrate for beta amylase and other glycosidases. It has been used as a fluorogenic substrate for food testing and as a chromogenic substrate for environmental testing. 4NPB has also been used in diagnostic applications such as enzyme substrates, ligands, and chemiluminescent substrates. This product is of high purity and quality with a conjugate that can be used in biotechnology applications.

Chromogenic substrate for esterase with C9 activity yielding a lapis colored precipitate upon cleavage.

Please enquire for more information about 2-Nitrophenyl 6-O-DMT-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-galactopyranoside is a chemiluminescent substrate that is used as a fluorescence indicator for the detection of β-galactosidase activities. This substrate has been shown to be useful for the detection of β-galactosidase in various types of samples such as culture media, environmental testing and food testing. 4NPTAG can also be used to detect β-galactosidase activity using fluorogenic substrates or chromogenic substrates. The enzyme activity can be detected by measuring the change in absorbance at 410 nm and the increase in luminescence at 460 nm.

Chromogenic substrate for beta-glucuronidase

L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate for the detection of aminoglycoside antibiotics. It can be used to determine the concentration of aminoglycosides in blood, urine, and serum. The chemiluminescence reaction is dependent on the presence of oxygen. This product has been shown to have a high quality and purity with environmental testing and staining properties. L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy--a--D--glucopyranoside has also been shown to bind ligands such as porphyrins, bilirubin, or hemin with bioluminescence or chromogenic substrates in diagnostics or culture media for food testing. L--2--Chloro--4--

4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-benzylidene-b-D-glucopyranoside is a high purity, high quality 4nitrophenol derivative that is used as a food testing substrate. It is also used for the detection of fluorescence in environmental testing. This product can be conjugated with ligands for use in diagnostic assays.