Chromogenic Substrates

Naphthol AS-OL is a widely used chromogenic enzyme substrate that forms a colored product upon reaction with specific enzymes. It is commonly employed in histochemical and cytochemical staining techniques to visualize enzyme activity in cells and tissues. Naphthol AS-OL is particularly useful for detecting the presence of enzymes such as alkaline phosphatase, acid phosphatase, and esterases. The resulting colored product can be easily observed under a microscope, allowing researchers to study the distribution and localization of these enzymes in various biological samples.

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is a chromogenic substrate used to detect the enzymatic activity of beta-galactosidase-6-phosphatase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is used in diagnostic assays such as lysosomal storage disorder.

Chromogenic substrate for neuraminidase yielding a blue precipitate upon cleavage.

Substrate for beta-galactosidase

4-Nitrophenyl decanoate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl decanoate is used in different applications such as the Pancreatic insufficiency diagnosis, lipid metabolism studies

5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt anhydrous is a potent inhibitor of histidine decarboxylase (HDC). It is used in the histochemical staining of dehydrogenase and effector proteins in urine samples. This compound has been shown to inhibit the growth of macroalgae and can be used as a selective agent for phylogenetic tree construction. 5BDG,Sr with its high specificity for HDC, also inhibits cell proliferation in plant cells and ovules.

Aldol® 455 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase producing bacteria grown in liquid or solid media, yielding a yellow color and green to orange fluorescence. Aldol® 455 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

5-Iodo-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase.

Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.

A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.

Aldol® 470 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase, yielding orange-yellow coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 470 L-alanine amide can also be used in enzymatic assays or within live bacterial cultures. Aldol® 470 L-alanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

2-Chloro-4-nitrophenyl b-D-xylobioside is a chemiluminescent substrate that is used as a diagnostic agent. It has been used in the identification of gram positive and gram negative bacteria, which are differentiated by the color of the reaction. This substrate has also been used to detect the presence of certain enzymes produced by bacteria. 2-Chloro-4-nitrophenyl b-D-xylobioside has shown to be an effective enzyme substrate for detecting bacterial growth in culture media and is often used as a fluorogenic substrate in food testing.

5-Bromo-4-chloro-3-indolyl acetate (5BCIA) is an enzyme substrate that has been shown to inhibit the activity of a number of enzymes, such as proteases and phosphatases. It has also been shown to inhibit the growth of a number of human pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. 5BCIA inhibits bacterial growth by binding to the active site of the enzyme phosphatase and preventing it from hydrolyzing a phosphate group from ATP. This process is necessary for energy metabolism in all living cells. 5BCIA has been shown to be effective against methicillin resistant Staphylococcus aureus (MRSA).

2-(N-Hexadecanoylamino)-4-nitrophenylphosphocholine hydroxide, often abbreviated as HDA-PC, is a chromogenic substrate for acid sphingomyelinase enzyme. HDA-PC is used as a key substrate for measuring the activity of this enzyme, responsible for catalyzing the hydrolysis of sphingomyelin to ceramide. The activity assay for this measuring this enzyme's acitivy is to detect a deficiency (or lack) of the enzyme which is directly related to the Niemann-Pick disease.

5-Bromo-4-chloro-1-methyl-3-indolyl-α-D-glucopyranoside is a fluorescent substrate used for the detection of α-glucuronidase.

Aldol® 515 caprylate hydrochloride is used as a bioluminescent substrate for enzyme activity measurements and as a fluorogenic substrate for detecting C8/C9-esterases, lipase activity yielding a red color.

5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside is a fluorogenic substrate that can be used to measure the activity of bacterial esterases. The enzyme cleaves the xylose moiety from 5-bromo-4-chloro-3-indoxyl, yielding indoxyl as an intermediate product. This reaction can be catalyzed by esterases found in bacteria, which hydrolyze esters containing a single hydroxyl group at one end and a carboxylic acid or alcohol at the other end. Indoxyl is fluorescent and can be detected using a fluorimeter. 5BX is also chromogenic, so it can be used as an enzyme substrate for detecting beta galactosidase activity in bacteria such as Mycobacterium smegmatis or Escherichia coli. 5BX is also used to detect chemilumines

4-(Hydroxymethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is an enzyme substrate that can be used for bioluminescence, staining, and colorimetric assays. It has been shown to be a good ligand for enzyme substrates such as alkaline phosphatase, glucose oxidase, β-galactosidase, and horseradish peroxidase. This product is a high quality reagent that is suitable for use in diagnostics.

Please enquire for more information about Aldol® 584 beta-D-glucuronic acid, triethylammonium salt, Cymit Quimica Patent: EP 2427431 and US 8940909 including the price, delivery time and more detailed product information at the technical inquiry form on this page