Chromogenic Substrates

N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.

4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.

4-Nitrophenyl 2-azido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase. It is used in the food industry to test for the presence of milk in foods that are not supposed to contain it. This product also has many other applications, including as a diagnostic tool in clinical laboratories and as an environmental testing agent. The high purity and high quality of this product makes it ideal for use in conjugates with other proteins, such as enzymes or antibodies.

2-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate that is used in food testing as well as environmental testing. It has been shown to be a ligand for the enzyme beta-galactosidase. This product is also a chemiluminescent and bioluminescent substrate that can be used in diagnostics and culture media.

4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-galactosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the Fabry disease diagnosis, glycolipid metabolism studies

4-Nitrophenyl 6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside (4NBP) is a fluorogenic substrate for β-galactosidase. It has been used as an enzyme substrate in the detection of β-galactosidase activity. 4NBP is also used to detect the presence of galactose in culture media and as a chromogenic substrate in diagnostics.

Aldol® 515 beta-D-xylopyranoside is a chromogenic substrate that is used in food testing. It is an alternative to the more common glucose and sucrose, which are not suitable for some types of assays. Aldol® 515 beta-D-xylopyranoside is also used in diagnostics as a ligand for fluorescent antibody staining and chemiluminescence assays. It has been shown to increase the sensitivity of these tests by up to 10 times. The substrate has been tested extensively and is safe for use with a wide range of bacteria including E. coli, S. typhimurium, M. tuberculosis, and C. albicans at concentrations up to 100 mM without any inhibitory effects on growth or viability. END>.

Chromogenic substrate for α-glucosidase yielding a salmon-colored precipitate.

4-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a fluorescent substrate for the beta-glucuronidase enzyme. It can be used to detect the presence of this enzyme in bacteria and other microorganisms by means of fluorometric or colorimetric assays. It has been shown to have an excellent stability in a variety of culture media and food testing applications. This product is available in high purity and quality, with a CAS number of 18472-49-6.

4-Nitrophenyl-beta-L-fucopyranoside is a high-quality pNP enzyme substrate developed for the sensitive detection and quantification of Beta-L-fucosidase activity. The enzymatic hydrolysis of the substrate generates 4-nitrophenol, a yellow chromogenic component that can be conveniently measured spectrophotometrically, allowing for accurate determination of enzyme concentration and kinetics. This substrate finds applications in various research fields, diagnostics, and industrial enzyme production.

Aldol® 515 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase produced by microorganisms, grown in liquid or solid media yielding red color. In addition, red fluorescence is generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-alanine amide can be used under aerobic and anaerobic conditions.

chromogenic substrate for beta-glucuronidase

Chromogenic substrate targeting amylase and maltase

Modified naphtol AS substrate used in histochemical and cytochemical staining

4-Nitrophenyl 2-acetamido-3-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-b-D-glucopyranosyl)-2-deoxy--4,6--O--4--methoxybenzylidene--a--D--galactopyranoside is a chemiluminescent substrate for the enzyme beta galactosidase. It reacts with this enzyme to form 4 nitrophenol and acetamide. 4 Nitrophenyl 2 acetamido 3 O (3,4,6 tri O acetyl 2 deoxy 2 phthalimido b D glucopyranosyl) 2 deoxy 4 6 O 4 methoxybenzylidene a D galactopyranoside is used as a chromogenic substrate in diagnostics and as an environmental testing agent. It can be used as an imaging

Ostazin Brilliant Red H-3B is a water soluble, non-toxic, and non-hazardous substrate for enzymes that catalyze reactions involving the cleavage of β-D-glucosides. It is used as a chromogenic or fluorogenic substrate in diagnostic tests. Ostazin Brilliant Red H-3B can also be used in bioluminescence reaction with luciferase to measure ATP levels in living cells.

Chromogenic substrate for cellobiohydrolase and endoglucanase

Modified naphtol AS substrate used in histochemical and cytochemical staining

Aldol® 495 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding orange color as well as green fluorescence if a fluorescence enhancer or a suitable matrix are present. Aldol® 495 beta-D-galactopyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar plates. Aldol® 495 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

4-Bromo-2-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that reacts with an excess of luciferase to produce light. It is used as a reagent in the detection of ATP, NADH, and other compounds in biochemistry and biology. 4-Bromo-2-nitrophenyl b-D-galactopyranoside is also used in diagnostic kits for detecting bacterial or fungal contamination in food.

Aldol® 518 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase-producing bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 518 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside is a chemiluminescent substrate that is used in the detection of enzymes. It is also used to measure the activity of beta-glucosidase, alpha-galactosidase, and alkaline phosphatase. The product has been tested for environmental sensitivity and has not shown any effects on bacterial growth. 4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside can be used as a high quality stain for histological sections and immunohistochemical studies. This product has been tested for staining properties with different dyes and has shown no interference with the staining process.

Aldol 458 nonanoate is a high quality, chromogenic and fluorogenic substrate for use in enzymatic, chemiluminescent, and immunochemical reactions. Aldol 458 nonanoate is used as a chromogenic substrate for the detection of glucose oxidase activity in culture media and as a fluorogenic substrate for the detection of glucose oxidase activity in environmental testing. This product has CAS number 2484872-52-6.

4-Methyl-2-nitrophenyl b-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. It has a high purity and is of high quality. 4-Methyl-2-nitrophenyl b-D-galactopyranoside is used as an enzyme substrate in diagnostics, as a chromogenic substrate in staining, and as a conjugate in chemiluminescence. It can be used for the detection of β-galactosidase in culture media and for the detection of β-galactosidase in environmental testing. This chemical also has bioluminescent properties that make it useful for food testing.

This powerful chromogenic substrate is designed specifically for the assay and detection of a-L-fucosidase enzyme activity. With a unique combination of bromo, chloro, and indolyl moieties, it offers enhanced stability and promotes color development that is unparalleled in clarity and intensity. Ideal for biochemistry, molecular biology, and related life sciences applications, this substrate provides unmatched performance and reliability for all your a-L-fucosidase enzyme research needs.

2-Chloro-4-nitrophenyl b-D-lactoside is a chromogenic enzyme substrate used to study the activity of enzymes. It produces a color when hydrolyzed, which makes it a valuable tool in screening for enzyme activity and studying enzyme kinetics.

Aldol 457 Beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase, which is an enzyme that catalyzes the hydrolysis of the terminal galactose residue in D-galactose to form D-galactitol and D-glucose. Aldol 457 Beta-D-galactopyranoside is used as a reagent for the detection of beta-galactosidase activity in biological samples, such as cultured cells or tissues, and has been shown to be a ligand in binding studies with Galectin 1. This product is also used in diagnostic applications such as food testing, culture media, fluorogenic substrates, environmental testing and more.

Chromogenic substrate for β-D-glucosidase yielding a magenta precipitate. Differentiation of Salmonella spp. (negative) from other Enterobacteriaceae.

2-Naphthyl beta-D-galactopyranoside is a glycoside that is found in the flowers of the plant Eschscholzia californica. It is used as a localizer for bacterial strains and can be hydrolyzed by enzymes to release naphthalene. 2-Naphthyl beta-D-galactopyranoside has been shown to inhibit the growth of corynebacterium and diazonium salt, which may be due to its ability to bind to DNA and disrupt protein synthesis. The potential use of this molecule is for its insolubility, which prevents it from being absorbed by skin cells.

5-bromo-3-indolyl-b-d-glucopyranoside, also known as 5BIG, is an enzyme substrate for beta-glucosidase. It is used in bacterial assays for the identifcation of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. After cleavege, it gives an intense blue color.

5-Bromo-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in lysosomal storage disorder screening such as for Sanfilippo syndrome.

2-Chloro-4-nitrophenyl β-D-cellotrioside is a chromogenic substrate use in cellulase activity assays. This enzyme, which is responsible to break down cellulose, hydrolyzes 2-chloro-4-nitrophenyl beta-D-cellotrioside and releases 2-chloro-4-nitrophenol than can be quantified spectrophotometrically. The use of 2-chloro-4-nitrophenyl beta-D-cellotrioside as substrate for enzymatic assays allows detecting bacterial cellulose in urine tract infections in the biofilm state.

5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.

Chromogenic substrate for α-amylase yielding a yellow solution upon cleavage.

Used as substrate for chemiluminescent assay of alkaline phosphatase (ALP) to produce a blue colour precipitate when cleaved.

Chromogenic substrate for N-acetylgalactosaminase

3-Indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a substrate used for the detection of the enzyme N-acetylglucosaminidase (NAG). The enzyme hydrolyzes the NAG conjugate, releasing indole that can be detected through a colorimetric assay. This method is commonly used in clinical chemistry to evaluate kidney function, as NAG is excreted in urine and increased levels may indicate renal injury or disease. The indole NAG assay is also used in microbiology to detect the presence of NAG-producing bacteria.

3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.

5-Bromo-6-chloro-3-indoxyl phosphate, disodium salt monohydrate is a substrate used to visualize phosphatase activity via showing magenta colored end product when used with Nitroblue Tetrazolium.

4-Nitrophenyl beta-D-cellotrioside is a chromogenic substrate for cellulase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-cellotrioside is used in different applications such as the Biomass conversion research, paper industry applications

Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of beta-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of beta-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Chromogenic substrate for beta-glucanase

Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.

Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.

Monohydrate; beta-glucuronidase substrate; yellow/pink color Beta-glucuronidase activity can be measured in a colorimetric assay with phenolphthalein beta-D-glucuronide as substrate. Starting assay conditions can include 30 µg of enzyme-containing material, 0.6 mM phenolphthalein beta-D-glucuronide in 100 mM sodium phosphate buffer in a total volume of 100 µL and monitoring absorbance changes at 540 nm. Temperature incubation can be used to promote the enzymatic activity (Galindo, 2012).

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used to detect and measure the activity of glycosidase enzymes. Glycosidases are enzymes that hydrolyze glycosidic bonds in carbohydrates, and are produced by many organisms. The substrate is cleaved by glycosidase to release a chromophore, which can be quantified via spectrophotometry analysis. 2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the detection of NAGase activity in biological samples, such as serum, urine, and cell lysates. It is also used for the identification and characterization of glycosidases in microorganisms and the study of glycosylation-related processes in cells and tissues.

N-a-Boc-N-epsilon-cbz-L-lysine 4-nitroanilide is a compound that has been shown to regulate the production of angiotensin. It also acts as an inhibitor for the production of fibronectin, which is key in the regulation of inflammation and wound healing. N-a-Boc-N-epsilon-cbz-L-lysine 4 nitroanilide has been shown to be a trifunctional molecule and can yield three different products: oxychlorides, hydrated and solvates. This molecule also regulates phosphorus levels. There are many functions that this molecule performs, including binding with amino acids and regulating their function, which is why it is so important to study this molecule more closely.

4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.

5-Bromo-3-indolyl-a-D-galactopyranoside (5BIG) is a bioluminescent substrate that is used in various applications, including environmental testing, diagnostics, and the detection of bacterial activity. 5BIG is a ligand for luciferase enzymes and is used as a chromogenic substrate. It also has the ability to be conjugated with other molecules for use in enzyme substrates or fluorescence assays. 5BIG has been shown to be an effective fluorogenic substrate for enzymes such as alkaline phosphatase and beta-glucuronidase.

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.