Chromogenic Substrates

Substrate for alkaline phosphatase

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)-α-D-galactopyranoside is a chemiluminescent substrate that has been used in the detection of bacterial activity. It is used in the detection of bacterial activity through chemiluminescence and can be conjugated to an enzyme or protein for increased sensitivity. This product has been shown to have high purity and quality and is suitable for use in diagnostic applications.

Aldol® 518 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase-producing bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 518 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

2-Naphthyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside (NAG tetra acetate) is a chromogenic enzyme substrate commonly used in the detection of β-galactosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is frequently used in bacterial assays to monitor gene expression or as a tool for screening potential inhibitors of the enzyme.

3-Indoxyl caprylate is a chemiluminescent substrate that is used in the detection of bacteria. It has been shown to be a very sensitive and selective fluorogenic substrate for the detection of bacterial activity, which can be used with an appropriate enzyme reaction. 3-Indoxyl caprylate has also been shown to be a potent quencher of bioluminescence, and can be used as a chromogenic substrate in diagnostic assays. 3-Indoxyl caprylate is often used as a culture media supplement or food additive to enhance its growth properties. It has been found to have high purity and quality levels, making it suitable for use in diagnostics and research.

Chromogenic substrates targeting beta-xylosidase

2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

6-Fluoro-3-indoxyl-beta-D-galactopyranoside is a fluorescent chromogenic substrate that is commonly used for the detection and quantification of beta-galactosidase activity. When this substrate is hydrolyzed by beta-galactosidase, it produces a fluorescent blue product that can be easily detected and measured using a fluorometer or spectrophotometer. This substrate is often used in microbiology and molecular biology research to study gene expression and enzyme kinetics, and is also used in medical diagnostics as a marker for specific diseases and conditions.

2,4-Dinitrophenyl 2-deoxy-2-fluoro-beta-xylobioside, also known as DNFPX, is a substrate to study the activity of glycosidases. It is a derivative of xylobiose, which is a disaccharide composed of two xylose units. The 2-deoxy-2-fluoro modification on one of the xylose units makes the compound resistant to further hydrolysis by the enzyme, allowing it to be used as a reporter of enzyme activity. The 2,4-Dinitrophenyl group is used as a chromogenic group that enables rapid detection and measurement of the enzyme activity that can be quantitatively measured by monitoring the appearance of the yellow 2,4-dinitrophenyl group when cleavage of the glycosidic bond.

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate commonly used in enzyme assays for the detection and quantification of enzyme activity. It is a colorless substrate that is enzymatically converted into a yellowish product, the intensity of which is directly proportional to the enzyme activity present in the sample.

A substrate for visualising alkaline phosphatase activity. Produces a red colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

Chromogenic substrate for N-Acetyl-β-D-galactosaminidase yielding a salmon-colored precipitate.

4-Nitrophenyl hepta-O-acetyl-b-lactoside is a high-quality chromogenic pNP enzyme substrate that exhibits excellent sensitivity and specificity in enzyme assays. This synthetic compound is designed to react with β-galactosidase enzymes, generating a yellow product (4-nitrophenol) upon cleavage. The resulting color change allows for quantitative measurement of enzyme activity, providing valuable insights in various research applications including enzyme kinetics, screening of inhibitors, drug discovery, and molecular diagnostics. Our product ensures consistent results with minimal interference, making it a reliable choice for a wide range of biochemical applications.

Chromogenic substrate for beta-galactosidase

Alternative chromogenic substrate for alpha-D-galactosidase

Modified naphtol AS substrate used in histochemical and cytochemical staining

5-Iodo-3-indolyl-b-D-galactopyranoside-6-sulfate sodium salt is a high purity, water soluble fluorogenic substrate for the detection of beta-galactosidase activity. This product is used in food testing, environmental testing, and diagnostics. 5-Iodo-3-indolyl-b-D-galactopyranoside (5IIAG) is a beta galactosidase substrate that provides a convenient method for determining beta galactosidase activity. This product is also used as an indicator to measure the rate of hydrolysis of ester bonds in other compounds that contain acid functional groups such as glycerides and oils.

4-Nitrophenyl 4,6-benzylidene-alpha-D-maltoheptaoside is a chromogenic substrate for alpha-amylase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 4,6-benzylidene-alpha-D-maltoheptaoside is used in different applications such as the Starch digestion studies, industrial enzyme optimization

Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.

2-Naphthyl butyrate is used as a chromogenic substrate in hematological studies via measuring esterase activity. This type of assays are important as it can help in distinguishing acute leukemias with monocytic or granulocytic differentiation. After hydrolysis of 2-naphthyl butyrate by the esterase (for example acylase), b-napthol is released that can be quantified spectrophotometrically (Exc 320 nm; Em 410 nm).

6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a strong inhibitor of alpha-mannosidase. It has been shown to inhibit the growth of Mycobacterium tuberculosis and Streptococcus pneumoniae, which are both known to cause bacterial infections in humans. 6BNA inhibits the enzyme alpha-mannosidase, which hydrolyzes terminal α-(1,4)-linked mannose residues from oligosaccharides and glycoproteins. This drug has a high affinity for the active site of alpha-mannosidase and an IC50 value of 0.2 mM, making it an effective inhibitor.

5-Bromo-3-indolyl phosphate p-toluidine salt is a bioluminescent substrate that is used as a chemiluminescent and fluorogenic substrate in diagnostic assays. It is a conjugate of the enzyme 5-bromo-3-indolyl phosphate and p-toluidine. This compound has been shown to be an excellent substrate for the enzyme alkaline phosphatase, which catalyzes the production of light from 5-bromo-3-indolyl phosphate. In addition, this product has been shown to be a chromogenic substrate for horseradish peroxidase.

Chromogenic substrate for alkaline phosphatase

4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is a fluorescent substrate for the enzyme beta-glucosidase. It reacts with an acyl group at the C2 position of the glycoside moiety, producing an aldehyde group at the C1 position and a hydroxymethylene group at the C4 position. This reaction is catalyzed by beta-glucosidase, which cleaves the glycosidic linkage in sucrose and other disaccharides. The product can be detected using luminescence methods, such as chemiluminescence or bioluminescence. 4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is used to detect β glucosidase activity in bacteria. This substrate has been shown to be sensitive and specific for β glucosidase activity when combined with fluorescence detection technology

a-Naphthyl phosphate disodium salt hydrate is a highly water-soluble chromogenic substrate used to determine phosphatase activity in research and analytical applications, particularly for staining and differentiating acid and alkaline phosphatases.

2-Nitrophenyl alpha-D-galactopyranoside is a chromogenic substrate used to detect the enzymatic activity of alpha-galactosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl alpha-D-galactopyranoside is used in Fabry disease screening.

Substrate for determining hydrolytic activity of chitinase

4-Nitrophenyl b-D-glucuronide sodium salt is a high-quality chromogenic substrate specifically designed for the colorimetric detection and measurement of β-glucuronidase activity. Upon enzymatic cleavage, it releases the yellow 4-nitrophenol, enabling quantitative analysis via spectroscopy. This compound is conveniently provided as a sodium salt to ensure enhanced solubility, stability, and shelf life. It is an ideal choice for biochemical, research, and diagnostic applications requiring a reliable and efficient b-D-glucuronide substrate.

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

2-Nitrophenyl-beta-D-galactopyranoside is used as chromogenic substrate for quantitative studies and enzyme kinetics of beta-galactosidase. It is used in assays for quantification of the activity of beta-galactosidase reporter gene constructs in molecular biology. This test is routinely used in diagnostics for the identification of lactose-fermenting bacteria of the Enterobacteriaceae family.

5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is a chemiluminescent ligand that is used in the detection of DNA, RNA, and proteins. It has been shown to be an efficient fluorescence substrate for DNA and RNA polymerases. 5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is suitable for use in diagnostic tests for bacteria, fungi, viruses, parasites, and toxins. This compound has been extensively tested for toxicity and carcinogenicity.

Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.

2-Nitrophenyl 2,2',3,3',4'-penta-O-acetyl-b-D-xylobioside is an oligosaccharide that is used as a substrate for the transport of nucleic acid molecules. It can be used to study the function of membrane transport proteins and their homologues. This compound has been shown to inhibit the growth of corynebacterium glutamicum and trisaccharide emissions.

Chromogenic substrate targeting beta-D-xylosidase

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.

5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.

4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is a fluorogenic substrate used in the detection of alpha amylase. It reacts with alpha amylase to produce light, and can be detected by measuring the increase in light emission over time. 4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is an excellent substrate for use in both enzymatic assays and chemiluminescence assays. It can be conjugated to antibodies or other proteins and is stable in culture media at high pH. This product has been shown to produce a signal that is proportional to the concentration of alpha amylase. The enzyme substrate comes in high purity and high quality, with a ligand that has been shown to have good binding affinity.

Chromogenic substrate for alpha-galactosidase

6-Chloro-3-indoxyl caprylate is a high quality, enzyme substrate, chromogenic substrate, bioluminescence, chemiluminescence ligand, staining and diagnostics reagent. It has CAS No. 159954-35-5 and is used in food testing, culture media and environmental testing. 6-Chloro-3-indoxyl caprylate is also a fluorogenic substrate that is used for high purity applications such as conjugation with other molecules.

Aldol® 484 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-glucosidase activity. The colorless enzyme substrate is cleaved by bacteria producing ?-glucosidase in liquid or solid media, yielding intense red color and green fluorescent signal. Aldol® 484 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indolyl a-D-maltotrioside is an enzyme substrate that shows chemiluminescence. It is used in diagnostic assays to detect bacterial and fungal infections, as well as other diseases. The test uses 5-Bromo-4-chloro-3-indolyl a-D-maltotrioside as the substrate for the enzyme horseradish peroxidase. When light is applied, the substrate reacts with hydrogen peroxide in the presence of horseradish peroxidase to form colored products that can be detected by a luminometer.

Aldol® 515 beta-D-xylopyranoside is a chemiluminescent ligand that can be used for the detection of beta-xylosidase activity to yield a red color.

2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.

Growth medium for the detection of histamine-producing bacteria in food and water samples. The medium contains an indicator that turns orange/red (absorbance at 555 nm) due to metabolites released by histidine decarboxylase. The BCM® Histamine-producing bacteria Broth contains tryptone (FT09555) and yeast extract, together with the chromogenic agent. High-level histamine-producing bacteria yield orange to red coloration in less than 2 days and even after 24 hours, while cultures of other bacteria remain yellow.

5-Bromo-4-chloro-3-indolyl myo-inositol-1-phosphate ammonium is a fluorogenic substrate used in the detection of phosphatidylinositol phosphate kinases. It can be used as a chromogenic substrate for detecting phosphatidylinositol phosphate kinase activity in tissues, cells, and biological fluids. 5-Bromo-4-chloro-3-indolyl myo-inositol 1 phosphate ammonium is also used as a ligand to detect phosphatidylinositol phosphate kinase activity in vivo. This product is of very high purity and quality. 5-Bromo 4 chloro 3 indolyl myo inositol 1 phosphate ammonium can be used in Enzyme Assays, Diagnostics and Cultures Media.

Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-galactopyranoside is a chemiluminescent substrate for the detection of beta galactosidase. It is a white to light yellow powder with a molecular weight of 564.87 and an assay of ≥ 98% by HPLC. This product has been shown to be stable when stored at 4°C for up to three months.

8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.