CAS 71418-44-5: Monobromobimano

Bromobimane is a monobromobimane dye that has been used in the past to measure the biofilm accumulation in wastewater treatment systems. Bromobimane binds to the thiol groups on proteins, causing a change in fluorescence properties. The bromobimane dye can also be used to monitor the viability of cells by measuring mitochondrial membrane potential, which is an indicator of cell health. Bromobimane has been shown to have antioxidant properties and can reduce glutathione levels in biological samples. This dye has also been used as a marker for protein synthesis, as it binds to basic structural elements of proteins. Bromobimane is an analytical tool for detecting disulfide bonds, which are covalent bonds between two sulfur atoms.

Bromobimane, non-fluorescent alone, becomes fluorescent with thiols; used to measure blood sulfide.

3-(Bromomethyl)-2,5,6-Trimethylpyrazolo[1,2-A]Pyrazole-1,7-Dione

Monobromobimane is a fluorescent probe, which is synthesized chemically and often used in biochemical applications. It acts through its highly reactive bromine atom, allowing it to form a covalent bond with thiol groups. This property facilitates its function as a fluorescence-based sensor. Monobromobimane is primarily utilized in the detection and quantification of thiols in biological samples, such as proteins and peptides. Upon rapid reaction with free thiol groups, it forms a stable thioether bond, resulting in a shift in the fluorescent properties of the molecule. This enables sensitive fluorescence-based detection and quantification of thiol concentrations in complex biological matrices. In scientific research, monobromobimane's applications span various studies involving redox biology, protein thiol modification, and cellular response to oxidative stress. It is a valuable tool for investigating thiol-based biochemical pathways and can be utilized in both in vitro and in vivo settings, providing insights into the dynamics of thiol-containing molecules. Its specific reactivity and fluorescent characteristics make it a sought-after compound in enzymology and cellular biology research.