CAS 76823-03-5: 5-carboxifluoresceína
Descripción:
5-carboxifluoresceína es un colorante fluorescente comúnmente utilizado en diversas aplicaciones bioquímicas y analíticas, particularmente en microscopía de fluorescencia y citometría de flujo. Se caracteriza por su brillante fluorescencia verde, que se atribuye a su estructura de xanteno. El compuesto presenta un grupo ácido carboxílico que mejora su solubilidad en soluciones acuosas, lo que lo hace adecuado para estudios biológicos. Su estructura molecular permite una unión efectiva a proteínas y ácidos nucleicos, facilitando su uso como trazador o marcador en imágenes celulares. Además, 5-carboxifluoresceína se emplea a menudo en la síntesis de sondas fluorescentes y en el desarrollo de ensayos para detectar actividad enzimática. El colorante exhibe estabilidad bajo una variedad de condiciones de pH, aunque su fluorescencia puede verse afectada por factores ambientales como el pH y la fuerza iónica. En general, 5-carboxifluoresceína es una herramienta versátil en el campo de la bioquímica y la biología molecular, permitiendo a los investigadores visualizar y cuantificar procesos biológicos con alta sensibilidad.
Fórmula:C21H12O7
InChI:InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
Clave InChI:InChIKey=NJYVEMPWNAYQQN-UHFFFAOYSA-N
SMILES:O=C1OC2(C=3C1=CC(C(O)=O)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4
Sinónimos:- 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
- 3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid
- 3′,6′-Dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
- 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid
- 5-Fam
- Carboxyfluorescein,5-
- FAM (dye)
- Fam 494/520
- Flamma 456 Carboxylic acid
- Hydroxyfluorescein
- Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
- Ver más sinónimos
Ordenar por
Encontrado 12 productos.
5-Carboxyfluorescein
CAS:Fórmula:C21H12O7Pureza:>97.0%(T)(HPLC)Forma y color:Light yellow to Brown powder to crystalPeso molecular:376.325-FAM
CAS:5-FAM, a carboxylic acid, reacts with amines for biomolecule labeling; excites at 488 nm, high fluorescence, solubility, absorbance.Fórmula:C21H12O7Pureza:99.32%Forma y color:Orange SolidPeso molecular:376.325-Carboxyfluorescein
CAS:5-Carboxyfluorescein (5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5â end of oligonucleotides in the presence of a quencher at the 3â end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.Fórmula:C21H12O7Pureza:Min. 98 Area-%Forma y color:Yellow PowderPeso molecular:376.32 g/mol5-Carboxyfluorescein, single isomer, 97%
CAS:This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.Fórmula:C21H12O7Pureza:97%Forma y color:Powder or crystals or crystalline powder, Yellow to orangePeso molecular:376.323',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
CAS:Fórmula:C21H12O7Pureza:95%Forma y color:SolidPeso molecular:376.315779999999965-Carboxyfluorescein (Technical Grade)
CAS:Producto controladoApplications 5-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. Dyes and metabolites. References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992); Mattingly, P.: Bioconjgate Chem., 3, 430 (1992); Bertrand, R., et al.: Biochemistry, 34, 9500 (1995);Fórmula:C21H12O7Forma y color:Yellow To Dark YellowPeso molecular:376.325-Carboxyfluorescein, 95%
CAS:This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.Pureza:95%Forma y color:Powder, Yellow to orange5-Carboxy-fluorescein
CAS:Widely used green-fluorescent labeling dye.Fórmula:C21H12O7Pureza:> 98%Forma y color:Yellow PowderPeso molecular:376.325-Carboxyfluorescein
CAS:Fórmula:C21H12O7Pureza:≥ 99.0%Forma y color:Yellow to orange powderPeso molecular:376.33',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
CAS:Pureza:95.0%Forma y color:SolidPeso molecular:376.32000732421875
![3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2FIN%2Fthumb%2FDA008P3R.jpg&w=3840&q=75)
![3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F10%2Fthumb%2FF448180.jpg&w=3840&q=75)
