Sustratos de enzimas

D-Psicose 6-phosphate sodium is an analog from D-psicose, a rare, natural monosaccharide with 70% the sweetness of sucrose. It is used as a low-calorie sweetener in food industry. In research, it is used to investigate its effects on metabolism, diabetes, and other health conditions.

4-Methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside is a chromogenic substrate. It is used for the detection of glycoproteins and glycolipids in food testing, staining, environmental testing, and diagnostics. It can also be used as a fluorogenic substrate for the detection of glycoproteins and glycolipids in fluorescence microscopy. 4-methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside has been shown to be a ligand for the lectin receptor Galectin 9, which is involved in inflammation and cancer cell growth. 4-methylumbelliferyl 3-O-(N-acetylneuraminyl)-b-D-glucopyranoside has been shown to be an enzyme substrate for alkaline phosphatase, β

The Methionine Assay Kit (Fluorometric) is a kit containing the fluorogenic substrate needed for the determination of total methionine content. The kit is used to detect and quantify methionine in various food products, as well as in environmental samples. This assay can be used to measure free or protein-bound methionine. The fluorometric kit contains a conjugate that binds to the methionine molecule and provides a fluorescent signal proportional to the amount of bound methionine. The fluorescence intensity is measured on a fluorometer and converted into concentrations of methionine per gram of sample.

4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside is an efficient chromogenic pNP enzyme substrate ideal for monitoring enzyme activity and understanding carbohydrate metabolism. This galacto-glucoside compound is designed specifically for use with glycosidases such as hexosaminidases and delivers unparalleled performance in your enzymatic assays.

4-Methylumbelliferyl-alpha-L-rhamnopyranoside is a fluorogenic substrate for alpha-L-rhamnosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-alpha-L-rhamnopyranosideas a substrate for measuring the alpha-L-rhamnosidase activity is used for detection of bacterial infection.

Targets Factor C substrate and peptidase

Chromogenic substrate targeting beta- galactosidase and whose color is dependent on the metal chelating.Alizarin-2-beta-D-galactopyranoside is a chromogenic substrate for enzymes based on alizarin and has been reported for the detection of bacterial beta-galactosidase by researchers at the University of Northumbria and the Freeman Hospital, Newcastle upon Tyne in the UK (James, 2000).

Bioluminescent substrate for luciferase

2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.

Chromogenic substrate for β-D-fucosidase yielding a blue precipitate.

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is a chromogenic substrate used to detect the enzymatic activity of beta-galactosidase-6-phosphatase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is used in diagnostic assays such as lysosomal storage disorder.

4-Methylumbelliferyl beta-D-xylobioside is a fluorogenic substrate for xylanase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-xylobiosideas a substrate for measuring the xylanase activity is used for detecting bacterial infection and gut microbiome analysis.

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Chitin azure is a chromogenic substrate for chitinases. It is one of the few specific substrates for this enzyme that produces chito-oligosaccharides from chitin.

2-(2-Thiazolylazo)-p-cresol structure enables it to form complexes with certain metal ions, changing color in the process. This color change is highly useful in titrimetric analyses (quantitative chemical analysis method) to determine the concentration of metal ions in a solution.  The substrates are colored orange-yellow (absorbance maximum= 375 nm). After the enzymatic conversion, the resulting TAC shows an intense, soluble red-violet color (544 nm) in an alkaline medium (pH = 10.3).

Chromogenic substrate for neuraminidase yielding a blue precipitate upon cleavage.