Sustratos cromogénicos

Chromogenic substrate targeting the beta-galactosidase enzyme

​Aldol® 455 alpha-D-maltopyranoside is a chromogenic substrate developed by Cymit Quimica, designed for the detection of maltosidases.

Chromogenic substrate targeting lipase, C8 esterase

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside 6-sulphate (BCIGS) is a chemiluminescent substrate that reacts with an enzyme to produce light. It has been used for the detection of various substances, including 5'-nucleotidase, 5'-adenosine monophosphate phosphohydrolases, and alkaline phosphatase. BCIGS can be used as a chromogenic substrate in the fluorometric assay of glucose oxidase and galactose oxidase. BCIGS is also used as a ligand in immunological reactions such as immunodiffusion, immunoelectrophoresis, and radioimmunoassay.

5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for β-D-galactosidase, yielding a magenta precipitate and contained in a chromogenic culture media. It is an indolyl carbohydrate that is the alpha-D-mannoside of indoxyl, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively.

2-Nitrophenyl laurate is a fatty acid that is not able to be degraded and has a high affinity for magnetic particles. It can be used in the immobilization of bacteria, such as Stenotrophomonas maltophilia, which are resistant to many antibiotics. 2-Nitrophenyl laurate also inhibits the enzyme lipolytic enzymes, which are responsible for the degradation of phospholipids in the cell membrane. This inhibition may lead to an increase in bacterial susceptibility to antibiotics. 2-Nitrophenyl laurate is a synthetic substrate that can be used to study the regulation of lipolytic enzymes by regulatory proteins.

5-Bromo-4-chloro-3-indoxyl-a-D-xylopyranoside is a fluorogenic substrate that can be used in food testing and environmental testing. This product is a conjugate of the xylosidase enzyme (EC 3.2.1.37) with 5-bromo-4-chloroindoxyl, which has been shown to have a high affinity for the enzyme's active site and enzymatic activity. The purity of this product is > 95% and the CAS number is 68618-41-5.

Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.

N-Acetyl-5-bromo-3-indoxyl-2,3,4-tri-O-acetyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of bile acids and bilirubin in food testing. It is a conjugate of an acetylated 2,3,4 triacetyl glucuronic acid methyl ester with 5 bromo 3 indoxyl. This product has high purity and high quality.

5-Bromo-4-chloro-3-indoxyl butyrate is a colorimetric substrate used to detect and quantify esterase activity. Upon hydrolysis by esterases, it yields a blue-green dye, allowing the detection and quantification of enzyme activity. It is commonly used in assays for the screening of esterase-producing microorganisms and in research aimed at understanding the role of esterases in various cellular processes.

Water soluble, chromogenic substrate for visualizing alkaline phosphatase activity. Produces a blueinsoluble end product that is detected visually when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situhybridization, ELISAs and immunohistochemistry.

4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of α-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.

ONPG is a chromogenic substrate for beta-galactosidase and it is used for colorimetric assays and environmental impact assessment.

5-Bromo-3-indolyl phosphate disodium salt is a chemiluminescent substrate that is used for the detection of bacterial activity. It reacts with oxygen to produce light, which can be measured using a luminometer. It is also used as a chromogenic substrate for detection of bacterial activity in culture media. The enzyme reaction produces an intense blue fluorescence and is used for diagnostic purposes in food testing and medical diagnostics. 5-Bromo-3-indolyl phosphate disodium salt has been shown to be effective against many bacteria and fungi, including Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. This product is chemically stable at room temperature but should be stored at 4°C or below.

Beta-naphthyl stearate is a chromogenic substrate for measuring enzymatic activity.

Modified naphtol AS substrate used in histochemical and cytochemical staining

p-Nitrophenylphosphorylcholine is a substrate molecule for enzymes that catalyze phospholipid biosynthesis. It is the most common substrate used to study the enzyme activity of phosphatidylcholine synthase and phospholipase A2 in human serum. The reaction mechanism is not well understood, but it appears to involve autocatalysis and an initial nucleophilic attack by p-nitrophenolate on the carbonyl carbon atom of the choline moiety. p-Nitrophenylphosphorylcholine has been shown to exhibit receptor activity on cells, which may be due to its ability to bind with basic proteins. This compound can also inhibit meiosis in Drosophila melanogaster and increase levels of natriuretic peptides in rats, as well as cause cell lysis and polymerase chain reactions in mammalian cells.

X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing β-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-beta-D-galactoside, X-Gal) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate. X-Gal can also be used as a detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta-galactosidase, color develops within minutes to a few hours.

Aldol® 455 alpha-D-glucopyranoside is a synthetic fluorescent sugar that has been used in the diagnosis of bacterial infections. It has been used as a substrate for the enzyme beta-galactosidase and as a conjugate with anti-bacterial antibodies, which can be used to detect bacteria in urine and blood samples. Aldol® 455 alpha-D-glucopyranoside is also commonly used in the detection of bacteria in food and environmental samples. The product has a high purity level and quality.

This product is designed for the sensitive color detection andidentification of various bacteria, such as pathogenic strains of Listeria, Clostridium, Bacillus and Staphylococcus. 5-Bromo-4-chloro-3-indoxyl myo-inositol-1-phosphate (X-phos-inositol; X-IP) is a chromogenic indicator for phosphatidyl-inositol specific phospholipase C(PI-PLC, EC 4.6.1.13). The colorless product is cleaved by PI-PLC positive microorganisms in liquid media or on solid culture media and yields a blue-green color precipitate.

The maunfacturing of blue jeans started 150 years ago as workwear, and are found nowadays in almost every wardrobe. Their renowned indigo color tone is truly unique; nevertheless, the high demand for the dye poses serious sustainability and environmental problems connected with its manufacturing process. Recently, a group of scientists presented an alternative indigo dyeing method inspired by the indoxyl glucoconjugate present in the plant P.tinctorium. The researchers proposed as an alternative approach a biocatalytic process using recombinant E.coli to access water soluble β-gluco-indoxyl (indican). At the point of coloring, indican gets hydrolyzed by β-glucosidase enzymes forming indoxyl, which suddenly undergoes oxidation to leucoindigo and further crystallizes to indigo giving the typical blue denim its signature character.

6-Bromo-2-naphthyl-alpha-D-galactopyranoside is a chromogenic substrate used in enzyme assays to detect and measure the activity of enzymes that act on alpha-galactosides. It is a type of glycoside inhibitor of α-galactosidase and other glycosidases that hydrolyze galactose and other sugar derivatives. The product is used to study the changes in enzyme activity over time or in response to different conditions.

3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.

4-Nitrophenyl b-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase.

Aldol 495 acetate is a chromogenic substrate that contains the chemical compound Aldol. It is used in food testing, diagnostics, and environmental testing. Aldol 495 acetate can be used as a ligand to detect the presence of enzymes or bacteria in culture media and diagnostics. This product has been shown to have a high purity and quality.

Aldol® 470 acetate is a Fluorogenic substrate that is used in enzymatic reactions to produce a fluorescent product. Aldol® 470 acetate can be used as a ligand or a chromogenic substrate. This product is also used in diagnostic tests, such as conjugates and chemiluminescence, to detect the presence of certain enzymes or microorganisms. It can be used in culture media to measure the amount of certain enzymes, such as phosphatases or proteases, and has been shown to have high purity and quality. This product has CAS No. 1318785-37-3, 1318785-38-4, 1318785-39-5, 1318785-40-6 and 1318785-41-7.

Chromogenic substrate for ?-xylosidase

Chromogenic substrate for β-D-glucosidase. Yields a red precipitate upon cleavage.

4-Nitrophenyl 2,3-di-O-(b-D-glucopyranosyl)-b-D-glucopyranoside is a chromogenic substrate that can be used to detect the presence of β-glucuronidase in human and animal tissue. The compound is also a fluoroquinolone substrate for the detection of β-lactamase activity. 4NPBG is used in diagnostics as well as food testing, due to its ability to detect bacteria in both food and water samples. 4NPBG is a chemiluminescent substrate that emits light upon hydrolysis by β-glucuronidase or β-lactamase. The compound has been shown to be an excellent ligand for binding to protein A or protein G and can be used for immunoassays such as ELISA or Western blotting.

Aldol 544 beta-D-galactopyranoside is a chromogenic substrate for the detection of beta-galactosidase activity in food testing and diagnostics. Aldol 544 beta-D-galactopyranoside is used as a conjugate to detect the presence of an enzyme, such as beta-galactosidase, in biological samples. It has been used in environmental testing and as a fluorogenic substrate for the detection of β-galactosidase activity. Aldol 544 beta-D-galactopyranoside can be used as a chemiluminescent or bioluminescent substrate for the detection of β-galactosidase activity in diagnostic assays. This product has shown high purity and good quality based on its CAS number 2484872-92-4.

2-Nitrophenyl b-D-cellobioside heptaacetate is a chromogenic enzyme substrate used to assay for cellobiohydrolase activity. The substrate undergoes enzymatic hydrolysis, releasing 2-nitrophenol which can then be detected via absorbance at 400 nm. This type of substrate is also used for the analysis of cellulose and hemicelluloses in plant materials. The heptaacetate modification enhances solubility, making it ideal for use in organic solvents, and it also protects against unwanted hydrolysis by acid or alkali. The substrate can be used in a variety of applications, including enzyme assay development, screening for inhibitors or activators, and in the study of the mode of action and substrate specificity of cellulase enzymes.

4-Nitrophenyl b-D-galactopyranosiduronic acid is a chemiluminescent substrate for the detection of β-galactosidase. It is used for the determination of α-galactosidase activity in urine, feces, and blood. This product can be used as a chromogenic substrate for the detection of β-galactosidase in tissue culture medium. 4-Nitrophenyl b-D-galactopyranosiduronic acid is also a fluorogenic substrate which can be conjugated to an enzyme such as horseradish peroxidase or alkaline phosphatase.

4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.

5-Bromo-3-indolyl a-D-galactopyranoside is a fluorogenic substrate that can be used for the detection of beta-galactosidase activity. It is also used as an enzyme substrate in diagnostics and research, as well as a chromogenic substrate in staining. 5-Bromo-3-indolyl a-D-galactopyranoside has been shown to be an excellent conjugate for proteins, nucleic acids, and other biomolecules. This product can also be used in culture media for the cultivation of bacteria and yeast.

Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.

4-Nitrophenyl lacto-N-tetraoside is a chromogenic substrate for enzymes that cleave lactose-specific linkages, such as beta-galactosidases. Upon enzyme cleavage, the pNP group is released, resulting in a yellow-colored solution that can be spectrophotometrically quantified at 405 nm. This substrate is commonly used in detecting lactose hydrolysis activity, such as in dairy industry for production optimization studies, or in microbiology for bacterial lactose fermentation assays. Its tetrasaccharide structure resembles that of lactose and makes it a specific substrate for lactose-utilizing enzymes. The properties of 4-nitrophenyl lacto-N-tetraoside make it a useful tool in enzyme kinetics studies and screening of new lactose-utilizing microorganisms.

5-Bromo-4-chloro-3-indolyl β-D-glucuronide (BCIG) is an chromogenic enzyme substrate used to detect the presence of glucuronidase enzyme activity. The reaction occurs when BCIG reacts with the enzyme beta-glucuronidase yielding an intense blue color. It is used in a variety of biochemical and biomedical applications, such as the detection of pathogenic bacteria, the diagnosis of genetic disorders, and the isolation of recombinant proteins.

Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indoxyl-β-D-glucuronic acid sodium salt anhydrous is a bromoquinone that is highly specific for the β -glucuronidase enzyme. The enzyme is expressed in plants, rhizogenes and family Enterobacteriaceae, but not in mitochondria or Staphylococcus aureus. 5-Bromo-4-chloro-3-indoxyl-β -D glucuronic acid sodium salt anhydrous has been used to detect β -glucuronidase activity in plant cells, which can be measured by colorimetric techniques. It has also been used as a diagnostic aid to identify members of the family Enterobacteriaceae, including E. coli and Klebsiella pneumoniae, by using biochemical tests such as glucose oxidase and dehydrogenase activity on plates. 5Bromo 4 chlor

5-Cyano-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that can be used to measure the activity of esterases and other enzymes. It has been shown to be a potent inhibitor of human erythrocyte esterase. The compound is also a potent inducer of bioluminescence in bacteria, which can be used for diagnostic applications. 5-Cyano-3-indolyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of phosphatases and other enzymes. This product has an enzyme substrate function, which can be used for enzyme assays. 5CIGP is useful for measuring high levels of enzyme activity in the presence of strong inhibitors such as phenylmethylsulfonyl fluoride (PMSF).

5-Bromo-6-chloro-3-indoxyl-alpha-D-galactopyranoside is a fluorogenic substrate that can be used for the detection of enzyme activity. This substrate reacts with an enzyme to produce a product that emits light when excited by a laser. 5BClGALP has been shown to be useful in detecting the activity of enzymes such as beta-galactosidase, alpha-galactosidase, and beta-glucuronidase. As a result, this substrate is important for diagnostics, including bioluminescence testing, environmental testing, and food testing. 5BClGALP also has applications in chemistry and biochemistry research as an enzyme substrate or ligand in assays.

2-Chloro-4-nitrophenyl β-D-xylopyranoside is a fluorogenic substrate that can be used for the detection of β-glucuronidase activity in food testing, environmental testing, and diagnostics. It has been shown to be an effective substrate for detecting β-glucuronidase activity with a high degree of sensitivity and specificity. 2CNPX can also be used as a chromogenic substrate for the detection of β-glucuronidase activity in food testing and environmental testing. The conjugate form of this substrate is useful for immunochemical assays, such as enzyme linked immunosorbent assay (ELISA) or radioimmunoassay (RIA). This product is also used as a ligand in fluorescence resonance energy transfer (FRET) assays. 2CNPX can be used to detect β-glucuronidase activity by chemiluminescence

4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-b-L-fucopyranosyl)-6-O-benzoyl-a-D-galactopyranoside is a chemiluminescent substrate with a CAS number of 104341548. It is used as a fluorescent probe for the detection of alpha amylase, beta amylase and glucose oxidase. It can be used to detect bacteria in food. This substrate can be used in the diagnostic industry and is an important component for the production of various enzymes.

4-Nitrophenyl 2,6-di-O-benzoyl-a-D-galactopyranoside is a specialized chromogenic substrate for the accurate evaluation of α-galactosidase activity. Upon enzymatic hydrolysis, it generates the chromophore 4-nitrophenol, which can be conveniently quantified by absorbance spectroscopy. The substrate features a unique benzoylated galactopyranoside moiety for enhanced selectivity and compound stability. This high-quality substrate is perfect for various research, analytical, and diagnostic applications involving α-galactosidase detection or quantification.

Chromogenic substrate for beta-glucuronidase

5-Bromo-6-chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in mucopolysaccharidosis screening.

4-Nitrophenyl 6-O-trityl-a-D-glucopyranoside is a high-quality trityl pNP substrate that performs excellently in various enzymatic assays. Containing a trityl-protected sugar moiety, this substrate is perfect for investigating alpha-D-glucopyranoside-specific enzymes and providing precise data for your experiments.

A substrate for alkaline phosphatase yielding a blue insoluble product upon cleavage. Colour can be further enhanced when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. The micronized structure enhances solubility.

Chromogenic β-xylosidase substrate yielding a yellow solution upon cleavage.

5-Bromo-4-chloro-3-indolyl-D-thymidine-3'-phosphate cyclohexylammonium salt is a chromogenic substrate used to detect the specific enzymatic activity of alkaline phosphatase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl-D-thymidine-3'-phosphate cyclohexylammonium salt is used in immunohistochemistry (IHC) and Western blot detection, immunoassays used to detect proteins.

5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside is a chromogenic substrate for alpha-L-Fucosidase. It produces a green-blue color.

Chromogenic cephalosporin substrate for the detection of beta-lactamase activity

This product is a chromogenic substrate that is used in the detection of alkaline phosphatase and other enzymes. It is also used in food testing, staining, and diagnostics. This product has high purity with a CAS number of 125328-84-9.

3-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that is used for the detection of glycosidases. It has been shown to be a good ligand for the study of nucleic acid hybridization, and it can also be used in enzyme assays. 3NPPAG is available in high purity and high quality. This product has been tested for use in culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, CAS No. 61894-63-1, diagnostics, chromogenic substrate, enzyme substrate, food testing and conjugate applications.

6-Chloro-3-indoxyl butyrate is a chemiluminescent substrate that is used in diagnostics and research. It has been shown to be an excellent ligand for the detection of DNA and protein. 6-Chloro-3-indoxyl butyrate can also be used as a chromogenic substrate for the detection of alkaline phosphatase, acid phosphatase, or beta-galactosidase. This product is also used in food testing, enzyme substrates, and fluorogenic substrates.

4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is an advanced chromogenic pNP enzyme substrate that delivers unparalleled performance in enzymatic assays. This high-quality substrate is specifically designed for studying glycosidases and other enzymes involved in carbohydrate metabolism. Our 4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is synthesized with the highest quality materials to provide incredible accuracy and reliability in your research applications.

Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in ?-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

Chromogenic substrate for alkaline and acid phosphatase

The enzyme alkaline phosphatase hydrolyses 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) substrate to 5-bromo-4-chloro-3-indole that turns into a blue coloration upon oxidation.

5-Bromo-4-chloro-3-indolyl nonanoate is a fluorogenic substrate that is used in the detection of enzymes. The substrate reacts with various enzymes and produces a fluorescent product, which can be detected using a fluorimeter or luminometer. It has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase. This compound may also be used as an enzyme substrate for the detection of bacteria in culture media or for food testing. 5-Bromo-4-chloro-3 indolyl nonanoate is not mutagenic or genotoxic when tested on bacterial cells.

Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.

5-Bromo-4-chloro-3-indoxyl-alpha-D-xylopyranoside is an enzyme substrate that can be used in the detection of β-galactosidase activity. This product has been shown to be a good ligand for various xanthine oxidoreductases, including xanthine dehydrogenase and uricase. 5BBIX is also a chromogenic substrate for horseradish peroxidase and can be used to detect hydrogen peroxide in the presence of osmium tetroxide or potassium permanganate. 5BBIX has been extensively tested for use in diagnostics, food, and environmental testing.

Chromogenic substrate for beta-galactofuranosidase

5-Bromo-6-chloro-3-indoxyl butyrate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lipid digestion assays.

5-Bromo-4-chloro-3-indolyl acetate (5BCIA) is an enzyme substrate that has been shown to inhibit the activity of a number of enzymes, such as proteases and phosphatases. It has also been shown to inhibit the growth of a number of human pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. 5BCIA inhibits bacterial growth by binding to the active site of the enzyme phosphatase and preventing it from hydrolyzing a phosphate group from ATP. This process is necessary for energy metabolism in all living cells. 5BCIA has been shown to be effective against methicillin resistant Staphylococcus aureus (MRSA).

Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.

4-Nitrophenyl 2-acetamido-3-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic substrate designed for the detection and quantification of enzyme activity. This tetra-acetylated compound is specifically designed to be cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically, allowing for accurate and sensitive analysis of enzyme kinetics and inhibition studies.

Chromogenic substrate for β-D-glucosidase, yielding a blue precipitate and contained in a chromogenic culture media for the detection of Enterococci, Streptococci, Klebsiella species, Enterobacter species and Listeria species. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of the enzyme, beta-glucosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole; this immediately dimerises to give an intensely blue product.

Aldol® 518 choline phosphate is a fluorogenic substrate that is used in the detection of enzyme activity. It has been shown to be an effective substrate for enzymes such as beta-galactosidase and phosphatases, and can be used in the detection of bacterial growth. Aldol® 518 choline phosphate is also a chromogenic substrate for the detection of alkaline phosphatase. It has been shown to have high purity and bioluminescence properties, making it useful for detecting bacteria in culture media or environmental testing. The conjugate of Aldol® 518 choline phosphate with various ligands can be used to detect specific proteins or antibodies.

Chromogenic substrate targeting cellulase

4-Nitrophenyl-alpha-D-glucopyranoside, beta-Anomer is a chromogenic sugar that can be used as an indicator for the presence of 4-nitrophenol. It is a highly reactive compound that spontaneously reacts with nitrobenzene to form a dinitroaniline. This anomeric structure is easily oxidized by air and light to form the corresponding o-dinitroaniline. When this reaction occurs, this substance changes color from yellow to red.

2-Chloro-4-nitrophenyl a-D-fucopyranoside (CNPF) is a chromogenic enzyme substrate used primarily to detect fucosidase activity. CNPF is cleaved by the enzyme, which results in the release of a colored product that can be measured spectrophotometrically. Fucosidases are important enzymes that catalyze the hydrolysis of fucose-containing oligosaccharides, and CNPF is a commonly used substrate for the detection and quantification of these enzymes in various research studies.

6-Nitro-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with horseradish peroxidase to produce light. 6NIGP is used in the diagnostic field as a fluorogenic or chromogenic substrate for enzymes such as alkaline phosphatase, and as a staining agent for cells, tissues, and other biological materials. 6NIGP has been used in the detection of bacteria, fungi, parasites, and viruses. It is also used in food testing as an enzyme substrate. 6NIGP is purified by recrystallization from water or acetone and can be stored at −20°C.

[2-Methoxy-4-(2-nitrovinyl)phenyl] b-D-galactopyranoside (MNVP-gal) is a chromogenic enzyme substrate that is commonly used to detect the activity of beta-galactosidase in biological samples. MNVP-gal produces a distinctive magenta color when hydrolyzed by beta-galactosidase, making it particularly useful for applications where visual detection is necessary. This substrate is also resistant to endogenous proteases, making it well-suited for use in cellular and tissue preparations.

Nalpha-CBZ-L-Citrulline-p-nitroanilide is a synthetic chromogenic enzyme substrate used in protease enzymatic stutides. After hydrolysis, p-nitroaniline is released, which produces a yellow color detectable spectrophotometrically at 405 nm.

Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.

4-Nitrophenyl 4,6-O-benzylidene-3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a high purity fluorescent reagent that can be used as a substrate for many enzymes. It is also used in environmental testing and to detect the presence of nitrophenols in food. This product has CAS number 545414.

4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that is used in biochemistry as an enzyme substrate. It is a colorless solid that has high purity and high quality. This product is a ligand that binds to the enzyme beta glucosidase. The conjugate of this product with beta glucosidase can be used in diagnostics for the detection of glucose levels in culture media or food testing. This product also has chromogenic and chemiluminescent properties that are used in environmental testing and staining. CAS No.: 54323-05-8

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2,4-di-O-benzoyl-b-D-mannopyranoside is a bioactive metabolite that can be found in the gene expression profile of a human genome. It has been shown to have an effect on gene expression levels and cellular function. This metabolite is found in the environment and has been shown to be mediated by environmental factors. The high level of gene expression has been shown to be due to its ability to bind to DNA.

Chromogenic substrate yielding a soluble chromophore for the colorimetric assay of α-D-galactosidase upon cleavage. Used for the detection of hydrolases in tissues and membranes.

4-Nitrophenyl phosphate disodium salt hexahydrate is a substrate of the hydrolase alkaline phosphatase. The products of the enzymatic reaction are 4-nitrophenol and phosphate.

4-Nitrophenyl b-D-cellotetraoside is a chromogenic substrate utilized for the detection and quantification of cellulase enzyme activity. Cellulase enzymes hydrolyze the beta-1,4-glycosidic bonds of cellulose, a major component of plant cell walls. Upon cleavage by cellulase, it releases 4-nitrophenol, a compound that exhibits a yellow color and absorbs strongly at 405 nm, allowing for easy spectrophotometric measurements of cellulase activity levels in various sample types, including bacterial and fungal cultures, environmental samples, and industrial applications.

4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside is a fluorescent compound that is used in diagnostics and research. The compound is an enzyme substrate, a ligand for metal ions, and a chromogenic substrate. It can be used to test cultures of bacteria in culture media, as well as for food testing and environmental testing. 4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside has high purity and quality due to its high fluorescence intensity. This product has been shown to be highly stable in various conditions and may be used in conjugates with other compounds.

Chromogenic substrate for alpha-L-rhamnosidase

4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside is a chromogenic pNP enzyme substrate, which features a cyclohexylidene-protected mannopyranosyl core. Upon enzymatic hydrolysis by glycosidases or other related enzymes, it releases a yellow-colored product, 4-nitrophenol, that can be easily detected spectrophotometrically. This substrate is useful for studying enzyme specificity, kinetics, and inhibition, as well as for the screening of enzyme inhibitors, the identification of glycosidase-producing organisms, and the development of new analytical methods.

Celluchrome (short name for remazol brilliant blue carboxymethyl cellulose or RBB-CMC) is a chromogenic substrate for cellulases. With a higher sensitivity, RBB-CMC allows for the detection of the enzyme activity even when small amounts are present.

3-Indolyl b-D-cellotetraoside is a chemiluminescent substrate that is used as an indicator in the detection of aminoglycosides. The ligand is a cell wall component that has been shown to bind specifically to the active site of bacterial β-lactamases, which are enzymes that catalyze the hydrolysis of β-lactam antibiotics. 3-Indolyl b-D-cellotetraoside can be used for bacterial identification and enumeration, food testing, and enzyme substrate for diagnostics. It fluoresces in response to electron excitation with a wavelength of about 590 nm.