Sustratos cromogénicos

Chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. Used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.

2-Nitrophenyl 6-O-trityl-b-D-galactopyranoside is a chromogenic enzyme substrate used in assays to detect galactosidase activity. It is a derivative of galactose that can be hydrolyzed by galactosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. The trityl group helps to stabilize the molecule and prevent undesired hydrolysis during storage. This substrate is commonly used in the study of carbohydrate metabolism and in the detection of galactosidase activity in microorganisms.

b-D-glucosidase substrate for screening of enterococci and C.perferingens

Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranoside is an advanced bifunctional chromogenic pNP substrate specifically tailored for the simultaneous detection and quantification of multiple glycosidase enzymes. This dual-action substrate undergoes cleavage by two separate enzymatic reactions, which increases the range and sensitivity of detection. The resulting 4-Nitrophenol release can be easily measured spectrophotometrically, providing rapid and reliable enzyme analysis for advanced enzymology studies, enabling new discoveries in biochemistry and biotechnology.

4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-α-D-glucopyranoside is a high-quality chromogenic pNP enzyme substrate specifically designed to enable effective and accurate detection of enzyme activity. By utilizing this advanced substrate, researchers and professionals can perform a range of applications, including activity assays, enzyme inhibition studies, and substrate specificity profiling. This substrate's unique properties result in exceptional sensitivity and throughput, ensuring effective results for a wide variety of enzyme assays.

Chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis. Used in an colorimetric assay for the extracellular lipase of Pseudomonas fluorescens B52.

2-O-(4-Nitrophenyl)-a-D-N-glycolylneuraminic acid is commonly used to measure the activity of sialidase enzymes, specifically those that cleave glycoconjugates containing 2-O-(4-nitrophenyl)-alpha-D-N-glycolylneuraminic acid. The resulting product can be detected by measuring the absorbance at a specific wavelength using a spectrophotometer. This substrate offers a high degree of specificity for glycolylneuraminic acid-containing substrates and is useful for both research and diagnostic purposes.

4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is a chromogenic substrate for alpha-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is used in different applications such as the Pompe disease diagnosis, glycogen metabolism research

3-Indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that reacts with the enzyme β-galactosidase to produce a blue product. This product can be used to diagnose the presence of β-galactosidase in culture media, as well as in food and environmental testing. 3IAG is also a chromogenic substrate that can be used for detection of β-galactosidase activity.

6-Chloro-3-indoxyl choline phosphate is a chromogenic substrate for phosphatidylcholine-specific phospholipase C (PC-PLC) for the detection of mibrobial metabolites. The use of this specific substrate permits monitoring the activity of PC-PLC. This an important enzyme for the regulation of steady-state levels of phosphatidylcholine (PC) and diacylglycerol (DAG). Abnormally high levels of PC-PLC activity in various tissues have been linked to many disease progressions.

Chromogenic substrate for beta-D-galactosidase yielding a lapis colored precipitate. 5-Bromo-3-indoxyl-beta-D-galactopyranoside can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside as an alternative to 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), producing a darker blue color for detection of beta-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue). 5-Bromo-3-indoxyl-beta-D-galactopyranoside is also used in histochemistry to demonstrate beta-galactosidase activity in tissues.

Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.

4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.

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Crimson-Gal (Crimson-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a crimson colored precipitate. Crimson-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

Chromogenic substrate for β-D-glucosidase. Yields a red precipitate upon cleavage.

5-Bromo-6-chloro-3-indolyl b-D-glucuronide (5BCIG) is an antibiotic that inhibits bacterial growth by binding to the DNA gyrase and topoisomerase IV, which are enzymes that maintain the integrity of bacterial DNA. 5BCIG is detectable in tissues, and quantified in blood. It encodes a protein to the cell cytoplasm. The sensitivity of 5BCIG against clostridium perfringens was found to be 10 times higher than that of penicillin G or erythromycin. 5BCIG has optimal activity against clostridium perfringens when expressed functionally as a polypeptide.

Substrate for the detection of beta-D-fucosidase

Chromogenic substrate for cellulases; red color