Sustratos cromogénicos

2-Naphthyl butyrate is used as a chromogenic substrate in hematological studies via measuring esterase activity. This type of assays are important as it can help in distinguishing acute leukemias with monocytic or granulocytic differentiation. After hydrolysis of 2-naphthyl butyrate by the esterase (for example acylase), b-napthol is released that can be quantified spectrophotometrically (Exc 320 nm; Em 410 nm).

6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a strong inhibitor of alpha-mannosidase. It has been shown to inhibit the growth of Mycobacterium tuberculosis and Streptococcus pneumoniae, which are both known to cause bacterial infections in humans. 6BNA inhibits the enzyme alpha-mannosidase, which hydrolyzes terminal α-(1,4)-linked mannose residues from oligosaccharides and glycoproteins. This drug has a high affinity for the active site of alpha-mannosidase and an IC50 value of 0.2 mM, making it an effective inhibitor.

5-Bromo-3-indolyl phosphate p-toluidine salt is a bioluminescent substrate that is used as a chemiluminescent and fluorogenic substrate in diagnostic assays. It is a conjugate of the enzyme 5-bromo-3-indolyl phosphate and p-toluidine. This compound has been shown to be an excellent substrate for the enzyme alkaline phosphatase, which catalyzes the production of light from 5-bromo-3-indolyl phosphate. In addition, this product has been shown to be a chromogenic substrate for horseradish peroxidase.

Chromogenic substrate for alkaline phosphatase

4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is a fluorescent substrate for the enzyme beta-glucosidase. It reacts with an acyl group at the C2 position of the glycoside moiety, producing an aldehyde group at the C1 position and a hydroxymethylene group at the C4 position. This reaction is catalyzed by beta-glucosidase, which cleaves the glycosidic linkage in sucrose and other disaccharides. The product can be detected using luminescence methods, such as chemiluminescence or bioluminescence. 4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is used to detect β glucosidase activity in bacteria. This substrate has been shown to be sensitive and specific for β glucosidase activity when combined with fluorescence detection technology

a-Naphthyl phosphate disodium salt hydrate is a highly water-soluble chromogenic substrate used to determine phosphatase activity in research and analytical applications, particularly for staining and differentiating acid and alkaline phosphatases.

2-Nitrophenyl alpha-D-galactopyranoside is a chromogenic substrate used to detect the enzymatic activity of alpha-galactosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl alpha-D-galactopyranoside is used in Fabry disease screening.

Substrate for determining hydrolytic activity of chitinase

4-Nitrophenyl b-D-glucuronide sodium salt is a high-quality chromogenic substrate specifically designed for the colorimetric detection and measurement of β-glucuronidase activity. Upon enzymatic cleavage, it releases the yellow 4-nitrophenol, enabling quantitative analysis via spectroscopy. This compound is conveniently provided as a sodium salt to ensure enhanced solubility, stability, and shelf life. It is an ideal choice for biochemical, research, and diagnostic applications requiring a reliable and efficient b-D-glucuronide substrate.

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

2-Nitrophenyl-beta-D-galactopyranoside is used as chromogenic substrate for quantitative studies and enzyme kinetics of beta-galactosidase. It is used in assays for quantification of the activity of beta-galactosidase reporter gene constructs in molecular biology. This test is routinely used in diagnostics for the identification of lactose-fermenting bacteria of the Enterobacteriaceae family.

5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is a chemiluminescent ligand that is used in the detection of DNA, RNA, and proteins. It has been shown to be an efficient fluorescence substrate for DNA and RNA polymerases. 5-Bromo-4-chloro-3-indolyl 5-O-feruloyl a-L-arabinofuranoside is suitable for use in diagnostic tests for bacteria, fungi, viruses, parasites, and toxins. This compound has been extensively tested for toxicity and carcinogenicity.

Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.

2-Nitrophenyl 2,2',3,3',4'-penta-O-acetyl-b-D-xylobioside is an oligosaccharide that is used as a substrate for the transport of nucleic acid molecules. It can be used to study the function of membrane transport proteins and their homologues. This compound has been shown to inhibit the growth of corynebacterium glutamicum and trisaccharide emissions.

Chromogenic substrate targeting beta-D-xylosidase

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.

5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.

4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is a fluorogenic substrate used in the detection of alpha amylase. It reacts with alpha amylase to produce light, and can be detected by measuring the increase in light emission over time. 4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is an excellent substrate for use in both enzymatic assays and chemiluminescence assays. It can be conjugated to antibodies or other proteins and is stable in culture media at high pH. This product has been shown to produce a signal that is proportional to the concentration of alpha amylase. The enzyme substrate comes in high purity and high quality, with a ligand that has been shown to have good binding affinity.

Chromogenic substrate for alpha-galactosidase